Selective Culture Method for Hepatocyte-like Cells Differentiated from Human Induced Pluripotent Stem Cells
This study aimed to establish culture conditions which are able to give the differentiation of induced pluripotent (iPS) cells to hepatocytes. To this end, we examined the usefulness of a culture medium containing the components involved in the intermediary metabolism in the liver. More specifically...
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| Veröffentlicht in: | DRUG METABOLISM AND PHARMACOKINETICS 2014, Vol.29 (5), p.407-413 |
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| container_title | DRUG METABOLISM AND PHARMACOKINETICS |
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| creator | Kondo, Yuki Yoshihashi, Sachimi Mimori, Kayo Ogihara, Ruri Kanehama, Yoshinori Maki, Yoshiyuki Enosawa, Shin Kurose, Kouichi Iwao, Takahiro Nakamura, Katsunori Matsunaga, Tamihide |
| description | This study aimed to establish culture conditions which are able to give the differentiation of induced pluripotent (iPS) cells to hepatocytes. To this end, we examined the usefulness of a culture medium containing the components involved in the intermediary metabolism in the liver. More specifically, we examined the effect of the "modified L-15 medium" containing galactose, phenylalanine and ornitine, but deprived of glucose, tyrosine, arginine and pyruvic acid. The medium was altered according to changes in the expression of enzymes that participate in liver-specific pathways. After 25 days of differentiation, the differentiated cells expressed hepatocyte markers and drug-metabolizing enzymes. These expression levels were increased using modified L-15 medium. The survival of human fetal liver cells and the death of human fibroblasts were observed during culture in modified L-15 medium. Most of the cells that differentiated from human iPS cells using modified L-15 medium were stained by anti-human albumin antibody. These results suggest that iPS cells can be converted to high purity-differentiated hepatocytes by cultivating them in modified L-15 medium. |
| doi_str_mv | 10.2133/dmpk.DMPK-14-RG-022 |
| format | Article |
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To this end, we examined the usefulness of a culture medium containing the components involved in the intermediary metabolism in the liver. More specifically, we examined the effect of the "modified L-15 medium" containing galactose, phenylalanine and ornitine, but deprived of glucose, tyrosine, arginine and pyruvic acid. The medium was altered according to changes in the expression of enzymes that participate in liver-specific pathways. After 25 days of differentiation, the differentiated cells expressed hepatocyte markers and drug-metabolizing enzymes. These expression levels were increased using modified L-15 medium. The survival of human fetal liver cells and the death of human fibroblasts were observed during culture in modified L-15 medium. Most of the cells that differentiated from human iPS cells using modified L-15 medium were stained by anti-human albumin antibody. These results suggest that iPS cells can be converted to high purity-differentiated hepatocytes by cultivating them in modified L-15 medium.</description><identifier>ISSN: 1347-4367</identifier><identifier>EISSN: 1880-0920</identifier><identifier>DOI: 10.2133/dmpk.DMPK-14-RG-022</identifier><identifier>PMID: 24785642</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Cell Culture Techniques ; Cell Differentiation - drug effects ; Cell Survival - drug effects ; Culture Media - chemistry ; Culture Media - pharmacology ; cytochrome P450 ; differentiation ; energy sources ; hepatocytes ; Hepatocytes - cytology ; Hepatocytes - drug effects ; Hepatocytes - enzymology ; Hepatocytes - metabolism ; Humans ; induced pluripotent stem cells ; Induced Pluripotent Stem Cells - cytology ; Induced Pluripotent Stem Cells - drug effects ; selection medium</subject><ispartof>DRUG METABOLISM AND PHARMACOKINETICS, 2014, Vol.29 (5), p.407-413</ispartof><rights>2014 The Japanese Society for the Study of Xenobiotics</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c614t-27b68cbb3e36c272613465a3ccf1295e04454bb20d8b71d08a44cedf4a7fcb0f3</citedby><cites>FETCH-LOGICAL-c614t-27b68cbb3e36c272613465a3ccf1295e04454bb20d8b71d08a44cedf4a7fcb0f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24785642$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kondo, Yuki</creatorcontrib><creatorcontrib>Yoshihashi, Sachimi</creatorcontrib><creatorcontrib>Mimori, Kayo</creatorcontrib><creatorcontrib>Ogihara, Ruri</creatorcontrib><creatorcontrib>Kanehama, Yoshinori</creatorcontrib><creatorcontrib>Maki, Yoshiyuki</creatorcontrib><creatorcontrib>Enosawa, Shin</creatorcontrib><creatorcontrib>Kurose, Kouichi</creatorcontrib><creatorcontrib>Iwao, Takahiro</creatorcontrib><creatorcontrib>Nakamura, Katsunori</creatorcontrib><creatorcontrib>Matsunaga, Tamihide</creatorcontrib><creatorcontrib>Graduate School of Marine Science and Technology</creatorcontrib><creatorcontrib>COSMO BIO Co</creatorcontrib><creatorcontrib>Department of Food Science and Technology</creatorcontrib><creatorcontrib>Department of Clinical Pharmacy</creatorcontrib><creatorcontrib>Division of Medicinal Safety Science</creatorcontrib><creatorcontrib>Graduate School of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>National Center for Child Health and Development</creatorcontrib><creatorcontrib>Nagoya City University</creatorcontrib><creatorcontrib>Faculty of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>Tokyo University of Marine Science and Technology</creatorcontrib><creatorcontrib>Primary Cell Division</creatorcontrib><creatorcontrib>Educational Research Center for Clinical Pharmacy</creatorcontrib><creatorcontrib>National Institute of Health Sciences</creatorcontrib><creatorcontrib>Division for Advanced Medical Services</creatorcontrib><creatorcontrib>Ltd</creatorcontrib><title>Selective Culture Method for Hepatocyte-like Cells Differentiated from Human Induced Pluripotent Stem Cells</title><title>DRUG METABOLISM AND PHARMACOKINETICS</title><addtitle>Drug Metab Pharmacokinet</addtitle><description>This study aimed to establish culture conditions which are able to give the differentiation of induced pluripotent (iPS) cells to hepatocytes. 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These results suggest that iPS cells can be converted to high purity-differentiated hepatocytes by cultivating them in modified L-15 medium.</description><subject>Cell Culture Techniques</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Culture Media - chemistry</subject><subject>Culture Media - pharmacology</subject><subject>cytochrome P450</subject><subject>differentiation</subject><subject>energy sources</subject><subject>hepatocytes</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - enzymology</subject><subject>Hepatocytes - metabolism</subject><subject>Humans</subject><subject>induced pluripotent stem cells</subject><subject>Induced Pluripotent Stem Cells - cytology</subject><subject>Induced Pluripotent Stem Cells - drug effects</subject><subject>selection medium</subject><issn>1347-4367</issn><issn>1880-0920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhiMEoqXwC5BQjlxS_BUne-CAtmW3ohVVC2fLdsbCXScOtlOp_55ZpXDk4rHsZ96Zeaeq3lNyzijnn4ZxPpxf3Nx-a6ho7nYNYexFdUr7njRkw8hLvHPRNYLL7qR6k_MDIZy3gr2uTpjo-lYKdlod7iGALf4R6u0SypKgvoHyKw61i6new6xLtE8FmuAPiEAIub7wzkGCqXhdAMEUx3q_jHqqr6Zhsfh0G5bk51iQqe8LjGvi2-qV0yHDu-d4Vv38evlju2-uv--utl-uGyupKA3rjOytMRy4tKxjEseQrebWOso2LRAhWmEMI0NvOjqQXguBRZ3QnbOGOH5WfVx15xR_L5CLGn222IGeIC5ZUUl72krJGaJ8RW2KOSdwak5-1OlJUaKOLqujy-rosqJC3e0UuoxZH54LLGaE4V_OX1sR2K0A_nqrQ5yCn0A9xCVNOLmykY5QtFGMoCohbENaDBtFBOnwoNiawHUdlT6vSoCGPXpIKlsPE87rE-5NDdH_t9U_eQqmnQ</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Kondo, Yuki</creator><creator>Yoshihashi, Sachimi</creator><creator>Mimori, Kayo</creator><creator>Ogihara, Ruri</creator><creator>Kanehama, Yoshinori</creator><creator>Maki, Yoshiyuki</creator><creator>Enosawa, Shin</creator><creator>Kurose, Kouichi</creator><creator>Iwao, Takahiro</creator><creator>Nakamura, Katsunori</creator><creator>Matsunaga, Tamihide</creator><general>Elsevier Ltd</general><general>Japanese Society for the Study of Xenobiotics</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2014</creationdate><title>Selective Culture Method for Hepatocyte-like Cells Differentiated from Human Induced Pluripotent Stem Cells</title><author>Kondo, Yuki ; 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To this end, we examined the usefulness of a culture medium containing the components involved in the intermediary metabolism in the liver. More specifically, we examined the effect of the "modified L-15 medium" containing galactose, phenylalanine and ornitine, but deprived of glucose, tyrosine, arginine and pyruvic acid. The medium was altered according to changes in the expression of enzymes that participate in liver-specific pathways. After 25 days of differentiation, the differentiated cells expressed hepatocyte markers and drug-metabolizing enzymes. These expression levels were increased using modified L-15 medium. The survival of human fetal liver cells and the death of human fibroblasts were observed during culture in modified L-15 medium. Most of the cells that differentiated from human iPS cells using modified L-15 medium were stained by anti-human albumin antibody. These results suggest that iPS cells can be converted to high purity-differentiated hepatocytes by cultivating them in modified L-15 medium.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>24785642</pmid><doi>10.2133/dmpk.DMPK-14-RG-022</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1347-4367 |
| ispartof | DRUG METABOLISM AND PHARMACOKINETICS, 2014, Vol.29 (5), p.407-413 |
| issn | 1347-4367 1880-0920 |
| language | eng |
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| subjects | Cell Culture Techniques Cell Differentiation - drug effects Cell Survival - drug effects Culture Media - chemistry Culture Media - pharmacology cytochrome P450 differentiation energy sources hepatocytes Hepatocytes - cytology Hepatocytes - drug effects Hepatocytes - enzymology Hepatocytes - metabolism Humans induced pluripotent stem cells Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - drug effects selection medium |
| title | Selective Culture Method for Hepatocyte-like Cells Differentiated from Human Induced Pluripotent Stem Cells |
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