Effects of the Biosynthesis and Signaling Pathway of Ecdysterone on Silkworm (Bombyx mori) Following Exposure to Titanium Dioxide Nanoparticles
Silkworm (Bombyx mori), a model Lepidoptera insect, is economically important. Its growth and development are regulated by endogenous hormones. During the process of transition from larvae to pupae, 20-hydroxyecdysone (20E) plays an important role. The recent surge in consumer products and applicati...
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| Veröffentlicht in: | Journal of chemical ecology 2014-08, Vol.40 (8), p.913-922 |
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| creator | Li, Fanchi Gu, Zhiya Wang, Binbin Xie, Yi Ma, Lie Xu, Kaizun Ni, Min Zhang, Hua Shen, Weide Li, Bing |
| description | Silkworm (Bombyx mori), a model Lepidoptera insect, is economically important. Its growth and development are regulated by endogenous hormones. During the process of transition from larvae to pupae, 20-hydroxyecdysone (20E) plays an important role. The recent surge in consumer products and applications using metallic nanoparticles has increased the possibility of human or ecosystem exposure due to their unintentional release into the environment. We investigated the effects of exposure to titanium dioxide nanoparticles (TiO₂ NPs) on the action of 20E in B. mori. Titanium dioxide nanoparticle treatment shortened the molting duration by 8 hr and prolonged the molting peak period by 10 %. Solexa sequencing profiled the changes in gene expression in the brain of fifth-instar B. mori in response to TiO₂NPS exposure for 72 hr, to address the effects on hormone metabolism and regulation. Thirty one genes were differentially expressed. The transcriptional levels of pi3k and P70S6K, which are involved in the target of the rapamycin (TOR) signaling pathway, were up-regulated. Transcriptional levels of four cytochrome P450 genes, which are involved in 20E biosynthesis, at different developmental stages (48, 96, 144, and 192 hr) at 5th instars of all displayed trends of increasing expression. Simultaneously, the ecdysterone receptors, also displayed increasing trends. The 20E titers at four developmental stages during the 5th instar were 1.26, 1.23, 1.72, and 2.16 fold higher, respectively, than the control group. These results indicate that feeding B. mori with TiO₂ NPs stimulates 20E biosynthesis, shortens the developmental progression, and reduces the duration of molting. Thus, application of TiO₂ NPs is of high significance for saving the labor force in sericulture, and our research provides a reference for the ecological problems in the field of Lepidoptera exposured to titanium dioxide nanoparticles. |
| doi_str_mv | 10.1007/s10886-014-0487-0 |
| format | Article |
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Its growth and development are regulated by endogenous hormones. During the process of transition from larvae to pupae, 20-hydroxyecdysone (20E) plays an important role. The recent surge in consumer products and applications using metallic nanoparticles has increased the possibility of human or ecosystem exposure due to their unintentional release into the environment. We investigated the effects of exposure to titanium dioxide nanoparticles (TiO₂ NPs) on the action of 20E in B. mori. Titanium dioxide nanoparticle treatment shortened the molting duration by 8 hr and prolonged the molting peak period by 10 %. Solexa sequencing profiled the changes in gene expression in the brain of fifth-instar B. mori in response to TiO₂NPS exposure for 72 hr, to address the effects on hormone metabolism and regulation. Thirty one genes were differentially expressed. The transcriptional levels of pi3k and P70S6K, which are involved in the target of the rapamycin (TOR) signaling pathway, were up-regulated. Transcriptional levels of four cytochrome P450 genes, which are involved in 20E biosynthesis, at different developmental stages (48, 96, 144, and 192 hr) at 5th instars of all displayed trends of increasing expression. Simultaneously, the ecdysterone receptors, also displayed increasing trends. The 20E titers at four developmental stages during the 5th instar were 1.26, 1.23, 1.72, and 2.16 fold higher, respectively, than the control group. These results indicate that feeding B. mori with TiO₂ NPs stimulates 20E biosynthesis, shortens the developmental progression, and reduces the duration of molting. Thus, application of TiO₂ NPs is of high significance for saving the labor force in sericulture, and our research provides a reference for the ecological problems in the field of Lepidoptera exposured to titanium dioxide nanoparticles.</description><identifier>ISSN: 0098-0331</identifier><identifier>EISSN: 1573-1561</identifier><identifier>DOI: 10.1007/s10886-014-0487-0</identifier><identifier>PMID: 25139758</identifier><language>eng</language><publisher>Boston: Springer-Verlag</publisher><subject>Agriculture ; Animals ; Biochemistry ; Biological Microscopy ; Biomedical and Life Sciences ; Biosynthesis ; Bombyx - drug effects ; Bombyx - growth & development ; Bombyx - physiology ; Bombyx mori ; brain ; Butterflies & moths ; Chemical ecology ; Consumer products ; cytochrome P-450 ; Developmental stages ; ecdysterone ; Ecdysterone - biosynthesis ; Ecdysterone - metabolism ; Ecology ; Economic importance ; ecosystems ; Entomology ; gene expression regulation ; genes ; hormone metabolism ; Hormones ; humans ; instars ; labor force ; Larva - drug effects ; Larva - growth & development ; Larva - physiology ; Larvae ; Lepidoptera ; Life Sciences ; Metal Nanoparticles - toxicity ; molting ; Nanoparticles ; phosphatidylinositol 3-kinase ; pupae ; receptors ; sericulture ; Signal Transduction ; Silk ; silkworms ; Titanium ; Titanium - toxicity ; Titanium dioxide ; transcription (genetics)</subject><ispartof>Journal of chemical ecology, 2014-08, Vol.40 (8), p.913-922</ispartof><rights>Springer Science+Business Media New York 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-81a35cf98dc812771a3e71ea59d83f4f7cdb55f9b2b928eae59209ce00adcbf13</citedby><cites>FETCH-LOGICAL-c499t-81a35cf98dc812771a3e71ea59d83f4f7cdb55f9b2b928eae59209ce00adcbf13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10886-014-0487-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10886-014-0487-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25139758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Fanchi</creatorcontrib><creatorcontrib>Gu, Zhiya</creatorcontrib><creatorcontrib>Wang, Binbin</creatorcontrib><creatorcontrib>Xie, Yi</creatorcontrib><creatorcontrib>Ma, Lie</creatorcontrib><creatorcontrib>Xu, Kaizun</creatorcontrib><creatorcontrib>Ni, Min</creatorcontrib><creatorcontrib>Zhang, Hua</creatorcontrib><creatorcontrib>Shen, Weide</creatorcontrib><creatorcontrib>Li, Bing</creatorcontrib><title>Effects of the Biosynthesis and Signaling Pathway of Ecdysterone on Silkworm (Bombyx mori) Following Exposure to Titanium Dioxide Nanoparticles</title><title>Journal of chemical ecology</title><addtitle>J Chem Ecol</addtitle><addtitle>J Chem Ecol</addtitle><description>Silkworm (Bombyx mori), a model Lepidoptera insect, is economically important. Its growth and development are regulated by endogenous hormones. During the process of transition from larvae to pupae, 20-hydroxyecdysone (20E) plays an important role. The recent surge in consumer products and applications using metallic nanoparticles has increased the possibility of human or ecosystem exposure due to their unintentional release into the environment. We investigated the effects of exposure to titanium dioxide nanoparticles (TiO₂ NPs) on the action of 20E in B. mori. Titanium dioxide nanoparticle treatment shortened the molting duration by 8 hr and prolonged the molting peak period by 10 %. Solexa sequencing profiled the changes in gene expression in the brain of fifth-instar B. mori in response to TiO₂NPS exposure for 72 hr, to address the effects on hormone metabolism and regulation. Thirty one genes were differentially expressed. The transcriptional levels of pi3k and P70S6K, which are involved in the target of the rapamycin (TOR) signaling pathway, were up-regulated. Transcriptional levels of four cytochrome P450 genes, which are involved in 20E biosynthesis, at different developmental stages (48, 96, 144, and 192 hr) at 5th instars of all displayed trends of increasing expression. Simultaneously, the ecdysterone receptors, also displayed increasing trends. The 20E titers at four developmental stages during the 5th instar were 1.26, 1.23, 1.72, and 2.16 fold higher, respectively, than the control group. These results indicate that feeding B. mori with TiO₂ NPs stimulates 20E biosynthesis, shortens the developmental progression, and reduces the duration of molting. Thus, application of TiO₂ NPs is of high significance for saving the labor force in sericulture, and our research provides a reference for the ecological problems in the field of Lepidoptera exposured to titanium dioxide nanoparticles.</description><subject>Agriculture</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological Microscopy</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Bombyx - drug effects</subject><subject>Bombyx - growth & development</subject><subject>Bombyx - physiology</subject><subject>Bombyx mori</subject><subject>brain</subject><subject>Butterflies & moths</subject><subject>Chemical ecology</subject><subject>Consumer products</subject><subject>cytochrome P-450</subject><subject>Developmental stages</subject><subject>ecdysterone</subject><subject>Ecdysterone - biosynthesis</subject><subject>Ecdysterone - metabolism</subject><subject>Ecology</subject><subject>Economic importance</subject><subject>ecosystems</subject><subject>Entomology</subject><subject>gene expression regulation</subject><subject>genes</subject><subject>hormone metabolism</subject><subject>Hormones</subject><subject>humans</subject><subject>instars</subject><subject>labor force</subject><subject>Larva - drug effects</subject><subject>Larva - growth & development</subject><subject>Larva - physiology</subject><subject>Larvae</subject><subject>Lepidoptera</subject><subject>Life Sciences</subject><subject>Metal Nanoparticles - toxicity</subject><subject>molting</subject><subject>Nanoparticles</subject><subject>phosphatidylinositol 3-kinase</subject><subject>pupae</subject><subject>receptors</subject><subject>sericulture</subject><subject>Signal Transduction</subject><subject>Silk</subject><subject>silkworms</subject><subject>Titanium</subject><subject>Titanium - toxicity</subject><subject>Titanium dioxide</subject><subject>transcription (genetics)</subject><issn>0098-0331</issn><issn>1573-1561</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kcFu1DAURS0EokPhA9iAJTZlEXiO47G9pGUKSBUgtV1bjmNPXZJ4aieayVf0l-soBSEWrGzL594nvYPQawIfCAD_mAgIsS6AVAVUghfwBK0I47QgbE2eohWAFAVQSo7Qi5RuAaBcC_YcHZWMUMmZWKH7jXPWDAkHh4cbi099SFOfb8knrPsGX_ptr1vfb_FPPdzs9TSTG9NMabAx9BaHPjPtr32IHT45DV09HXAXon-Pz0Pbhv0c3Rx2IY3R4iHgKz_o3o8d_uzDwTcWf9d92Ok4eNPa9BI9c7pN9tXjeYyuzzdXZ1-Lix9fvp19uihMJeVQCKIpM06KxghScp6flhOrmWwEdZXjpqkZc7Iua1kKqy2TJUhjAXRjakfoMTpZencx3I02Darzydi21b0NY1JkTQRhjAPL6Lt_0NswxryUTOU9Myg5mQvJQpkYUorWqV30nY6TIqBmW2qxpbItNdtSkDNvHpvHurPNn8RvPRkoFyDlr35r41-j_9P6dgk5HZTeRp_U9WWZAQBCGa0kfQCUzqrS</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>Li, Fanchi</creator><creator>Gu, Zhiya</creator><creator>Wang, Binbin</creator><creator>Xie, Yi</creator><creator>Ma, Lie</creator><creator>Xu, Kaizun</creator><creator>Ni, Min</creator><creator>Zhang, Hua</creator><creator>Shen, Weide</creator><creator>Li, Bing</creator><general>Springer-Verlag</general><general>Springer US</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20140801</creationdate><title>Effects of the Biosynthesis and Signaling Pathway of Ecdysterone on Silkworm (Bombyx mori) Following Exposure to Titanium Dioxide Nanoparticles</title><author>Li, Fanchi ; Gu, Zhiya ; Wang, Binbin ; Xie, Yi ; Ma, Lie ; Xu, Kaizun ; Ni, Min ; Zhang, Hua ; Shen, Weide ; Li, Bing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-81a35cf98dc812771a3e71ea59d83f4f7cdb55f9b2b928eae59209ce00adcbf13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Agriculture</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biological Microscopy</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Bombyx - drug effects</topic><topic>Bombyx - growth & development</topic><topic>Bombyx - physiology</topic><topic>Bombyx mori</topic><topic>brain</topic><topic>Butterflies & moths</topic><topic>Chemical ecology</topic><topic>Consumer products</topic><topic>cytochrome P-450</topic><topic>Developmental stages</topic><topic>ecdysterone</topic><topic>Ecdysterone - biosynthesis</topic><topic>Ecdysterone - metabolism</topic><topic>Ecology</topic><topic>Economic importance</topic><topic>ecosystems</topic><topic>Entomology</topic><topic>gene expression regulation</topic><topic>genes</topic><topic>hormone metabolism</topic><topic>Hormones</topic><topic>humans</topic><topic>instars</topic><topic>labor force</topic><topic>Larva - drug effects</topic><topic>Larva - growth & development</topic><topic>Larva - physiology</topic><topic>Larvae</topic><topic>Lepidoptera</topic><topic>Life Sciences</topic><topic>Metal Nanoparticles - toxicity</topic><topic>molting</topic><topic>Nanoparticles</topic><topic>phosphatidylinositol 3-kinase</topic><topic>pupae</topic><topic>receptors</topic><topic>sericulture</topic><topic>Signal Transduction</topic><topic>Silk</topic><topic>silkworms</topic><topic>Titanium</topic><topic>Titanium - toxicity</topic><topic>Titanium dioxide</topic><topic>transcription (genetics)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Fanchi</creatorcontrib><creatorcontrib>Gu, Zhiya</creatorcontrib><creatorcontrib>Wang, Binbin</creatorcontrib><creatorcontrib>Xie, Yi</creatorcontrib><creatorcontrib>Ma, Lie</creatorcontrib><creatorcontrib>Xu, Kaizun</creatorcontrib><creatorcontrib>Ni, Min</creatorcontrib><creatorcontrib>Zhang, Hua</creatorcontrib><creatorcontrib>Shen, Weide</creatorcontrib><creatorcontrib>Li, Bing</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Toxicology Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>https://resources.nclive.org/materials</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Journal of chemical ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Fanchi</au><au>Gu, Zhiya</au><au>Wang, Binbin</au><au>Xie, Yi</au><au>Ma, Lie</au><au>Xu, Kaizun</au><au>Ni, Min</au><au>Zhang, Hua</au><au>Shen, Weide</au><au>Li, Bing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of the Biosynthesis and Signaling Pathway of Ecdysterone on Silkworm (Bombyx mori) Following Exposure to Titanium Dioxide Nanoparticles</atitle><jtitle>Journal of chemical ecology</jtitle><stitle>J Chem Ecol</stitle><addtitle>J Chem Ecol</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>40</volume><issue>8</issue><spage>913</spage><epage>922</epage><pages>913-922</pages><issn>0098-0331</issn><eissn>1573-1561</eissn><abstract>Silkworm (Bombyx mori), a model Lepidoptera insect, is economically important. Its growth and development are regulated by endogenous hormones. During the process of transition from larvae to pupae, 20-hydroxyecdysone (20E) plays an important role. The recent surge in consumer products and applications using metallic nanoparticles has increased the possibility of human or ecosystem exposure due to their unintentional release into the environment. We investigated the effects of exposure to titanium dioxide nanoparticles (TiO₂ NPs) on the action of 20E in B. mori. Titanium dioxide nanoparticle treatment shortened the molting duration by 8 hr and prolonged the molting peak period by 10 %. Solexa sequencing profiled the changes in gene expression in the brain of fifth-instar B. mori in response to TiO₂NPS exposure for 72 hr, to address the effects on hormone metabolism and regulation. Thirty one genes were differentially expressed. The transcriptional levels of pi3k and P70S6K, which are involved in the target of the rapamycin (TOR) signaling pathway, were up-regulated. Transcriptional levels of four cytochrome P450 genes, which are involved in 20E biosynthesis, at different developmental stages (48, 96, 144, and 192 hr) at 5th instars of all displayed trends of increasing expression. Simultaneously, the ecdysterone receptors, also displayed increasing trends. The 20E titers at four developmental stages during the 5th instar were 1.26, 1.23, 1.72, and 2.16 fold higher, respectively, than the control group. These results indicate that feeding B. mori with TiO₂ NPs stimulates 20E biosynthesis, shortens the developmental progression, and reduces the duration of molting. Thus, application of TiO₂ NPs is of high significance for saving the labor force in sericulture, and our research provides a reference for the ecological problems in the field of Lepidoptera exposured to titanium dioxide nanoparticles.</abstract><cop>Boston</cop><pub>Springer-Verlag</pub><pmid>25139758</pmid><doi>10.1007/s10886-014-0487-0</doi><tpages>10</tpages></addata></record> |
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| subjects | Agriculture Animals Biochemistry Biological Microscopy Biomedical and Life Sciences Biosynthesis Bombyx - drug effects Bombyx - growth & development Bombyx - physiology Bombyx mori brain Butterflies & moths Chemical ecology Consumer products cytochrome P-450 Developmental stages ecdysterone Ecdysterone - biosynthesis Ecdysterone - metabolism Ecology Economic importance ecosystems Entomology gene expression regulation genes hormone metabolism Hormones humans instars labor force Larva - drug effects Larva - growth & development Larva - physiology Larvae Lepidoptera Life Sciences Metal Nanoparticles - toxicity molting Nanoparticles phosphatidylinositol 3-kinase pupae receptors sericulture Signal Transduction Silk silkworms Titanium Titanium - toxicity Titanium dioxide transcription (genetics) |
| title | Effects of the Biosynthesis and Signaling Pathway of Ecdysterone on Silkworm (Bombyx mori) Following Exposure to Titanium Dioxide Nanoparticles |
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