Photochemical internalization-mediated nonviral gene transfection: polyamine core-shell nanoparticles as gene carrier
The overall objective of the research was to investigate the utility of photochemical internalization (PCI) for the enhanced nonviral transfection of genes into glioma cells. The PCI-mediated introduction of the tumor suppressor gene phosphatase and tensin homolog (PTEN) or the cytosine deaminase (C...
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| Veröffentlicht in: | Journal of biomedical optics 2014-10, Vol.19 (10), p.105009-105009 |
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| container_title | Journal of biomedical optics |
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| creator | Zamora, Genesis Wang, Frederick Sun, Chung-Ho Trinidad, Anthony Kwon, Young Jik Cho, Soo Kyung Berg, Kristian Madsen, Steen J Hirschberg, Henry |
| description | The overall objective of the research was to investigate the utility of photochemical internalization (PCI) for the enhanced nonviral transfection of genes into glioma cells. The PCI-mediated introduction of the tumor suppressor gene phosphatase and tensin homolog (PTEN) or the cytosine deaminase (CD) pro-drug activating gene into U87 or U251 glioma cell monolayers and multicell tumor spheroids were evaluated. In the study reported here, polyamine-DNA gene polyplexes were encapsulated in a nanoparticle (NP) with an acid degradable polyketal outer shell. These NP synthetically mimic the roles of viral capsid and envelope, which transport and release the gene, respectively. The effects of PCI-mediated suppressor and suicide genes transfection efficiency employing either "naked" polyplex cores alone or as NP-shelled cores were compared. PCI was performed with the photosensitizer AlPcS2a and λ=670-nm laser irradiance. The results clearly demonstrated that the PCI can enhance the delivery of both the PTEN or CD genes in human glioma cell monolayers and multicell tumor spheroids. The transfection efficiency, as measured by cell survival and inhibition of spheroid growth, was found to be significantly greater at suboptimal light and DNA levels for shelled NPs compared with polyamine-DNA polyplexes alone. |
| doi_str_mv | 10.1117/1.JBO.19.10.105009 |
| format | Article |
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The PCI-mediated introduction of the tumor suppressor gene phosphatase and tensin homolog (PTEN) or the cytosine deaminase (CD) pro-drug activating gene into U87 or U251 glioma cell monolayers and multicell tumor spheroids were evaluated. In the study reported here, polyamine-DNA gene polyplexes were encapsulated in a nanoparticle (NP) with an acid degradable polyketal outer shell. These NP synthetically mimic the roles of viral capsid and envelope, which transport and release the gene, respectively. The effects of PCI-mediated suppressor and suicide genes transfection efficiency employing either "naked" polyplex cores alone or as NP-shelled cores were compared. PCI was performed with the photosensitizer AlPcS2a and λ=670-nm laser irradiance. The results clearly demonstrated that the PCI can enhance the delivery of both the PTEN or CD genes in human glioma cell monolayers and multicell tumor spheroids. The transfection efficiency, as measured by cell survival and inhibition of spheroid growth, was found to be significantly greater at suboptimal light and DNA levels for shelled NPs compared with polyamine-DNA polyplexes alone.</description><identifier>ISSN: 1083-3668</identifier><identifier>EISSN: 1560-2281</identifier><identifier>DOI: 10.1117/1.JBO.19.10.105009</identifier><identifier>PMID: 25341069</identifier><language>eng</language><publisher>United States: Society of Photo-Optical Instrumentation Engineers</publisher><subject>Cell Line, Tumor ; Cell Proliferation - drug effects ; Cell Proliferation - genetics ; Cell Survival - drug effects ; Cell Survival - genetics ; Cytosine Deaminase - genetics ; Cytosine Deaminase - metabolism ; Cytosine Deaminase - pharmacology ; Degradation ; Drug Carriers - chemistry ; Drug Carriers - pharmacology ; Drug Carriers - toxicity ; Genes ; Genetic Therapy ; Humans ; Irradiance ; Monolayers ; Nanoparticles ; Nanoparticles - chemistry ; Nanoparticles - toxicity ; Photochemical ; Photochemotherapy ; Photosensitizing Agents - chemistry ; Photosensitizing Agents - pharmacology ; Photosensitizing Agents - toxicity ; Polyamines - chemistry ; Polyamines - pharmacology ; Polyamines - toxicity ; PTEN Phosphohydrolase - genetics ; PTEN Phosphohydrolase - metabolism ; PTEN Phosphohydrolase - pharmacology ; Research Papers: General ; Spheroids ; Spheroids, Cellular ; Suppressors ; Transfection - methods ; Tumors</subject><ispartof>Journal of biomedical optics, 2014-10, Vol.19 (10), p.105009-105009</ispartof><rights>2014 Society of Photo-Optical Instrumentation Engineers (SPIE)</rights><rights>2014 Society of Photo-Optical Instrumentation Engineers (SPIE) 2014 Society of Photo-Optical Instrumentation Engineers</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c544t-5cae9b4b0f9b31973fb01a11e059e867a97e820e90c38fd806da7d46a8683cd23</citedby><cites>FETCH-LOGICAL-c544t-5cae9b4b0f9b31973fb01a11e059e867a97e820e90c38fd806da7d46a8683cd23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206751/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206751/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25341069$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zamora, Genesis</creatorcontrib><creatorcontrib>Wang, Frederick</creatorcontrib><creatorcontrib>Sun, Chung-Ho</creatorcontrib><creatorcontrib>Trinidad, Anthony</creatorcontrib><creatorcontrib>Kwon, Young Jik</creatorcontrib><creatorcontrib>Cho, Soo Kyung</creatorcontrib><creatorcontrib>Berg, Kristian</creatorcontrib><creatorcontrib>Madsen, Steen J</creatorcontrib><creatorcontrib>Hirschberg, Henry</creatorcontrib><title>Photochemical internalization-mediated nonviral gene transfection: polyamine core-shell nanoparticles as gene carrier</title><title>Journal of biomedical optics</title><addtitle>J. Biomed. Opt</addtitle><description>The overall objective of the research was to investigate the utility of photochemical internalization (PCI) for the enhanced nonviral transfection of genes into glioma cells. The PCI-mediated introduction of the tumor suppressor gene phosphatase and tensin homolog (PTEN) or the cytosine deaminase (CD) pro-drug activating gene into U87 or U251 glioma cell monolayers and multicell tumor spheroids were evaluated. In the study reported here, polyamine-DNA gene polyplexes were encapsulated in a nanoparticle (NP) with an acid degradable polyketal outer shell. These NP synthetically mimic the roles of viral capsid and envelope, which transport and release the gene, respectively. The effects of PCI-mediated suppressor and suicide genes transfection efficiency employing either "naked" polyplex cores alone or as NP-shelled cores were compared. PCI was performed with the photosensitizer AlPcS2a and λ=670-nm laser irradiance. The results clearly demonstrated that the PCI can enhance the delivery of both the PTEN or CD genes in human glioma cell monolayers and multicell tumor spheroids. The transfection efficiency, as measured by cell survival and inhibition of spheroid growth, was found to be significantly greater at suboptimal light and DNA levels for shelled NPs compared with polyamine-DNA polyplexes alone.</description><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Proliferation - genetics</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - genetics</subject><subject>Cytosine Deaminase - genetics</subject><subject>Cytosine Deaminase - metabolism</subject><subject>Cytosine Deaminase - pharmacology</subject><subject>Degradation</subject><subject>Drug Carriers - chemistry</subject><subject>Drug Carriers - pharmacology</subject><subject>Drug Carriers - toxicity</subject><subject>Genes</subject><subject>Genetic Therapy</subject><subject>Humans</subject><subject>Irradiance</subject><subject>Monolayers</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - toxicity</subject><subject>Photochemical</subject><subject>Photochemotherapy</subject><subject>Photosensitizing Agents - chemistry</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>Photosensitizing Agents - toxicity</subject><subject>Polyamines - chemistry</subject><subject>Polyamines - pharmacology</subject><subject>Polyamines - toxicity</subject><subject>PTEN Phosphohydrolase - genetics</subject><subject>PTEN Phosphohydrolase - metabolism</subject><subject>PTEN Phosphohydrolase - pharmacology</subject><subject>Research Papers: General</subject><subject>Spheroids</subject><subject>Spheroids, Cellular</subject><subject>Suppressors</subject><subject>Transfection - methods</subject><subject>Tumors</subject><issn>1083-3668</issn><issn>1560-2281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk9v1DAQxSMEoqXwBTigHLlkOxMnjs0BqRTKH1VqDyCOluNMuq6ydrCTSu2nx7spBVQEnGyNf-9pxvOy7DnCChGbQ1x9enO2QrnaFqAGkA-yfaw5FGUp8GG6g2AF41zsZU9ivAQAwSV_nO2VNasQuNzP5vO1n7xZ08YaPeTWTRScHuyNnqx3xYY6qyfqcufdlQ2JuCBH-RS0iz2ZLfMqH_1wrTc21Y0PVMQ1DUPutPOjDpM1A8Vcx0VodAiWwtPsUa-HSM9uz4Psy8m7z8cfitOz9x-Pj04LU1fVVNRGk2yrFnrZMpQN61tAjUhQSxK80bIhUQJJMEz0nQDe6aaruBZcMNOV7CB7vfiOc5tGMeRS54Mag93ocK28tur3F2fX6sJfqaoE3tSYDF7eGgT_baY4qY2NJs2nHfk5KmzST3JkTPwHmhbUNMDKf6McBdZQNlVCywU1wccYqL9rHkFtU6BQpRQolLvCLgVJ9OLXse8kP9aegMMFiKMldenn7c7j3y2__knxk7ux4z3Vrni0ZOD87cn997Hr2XdahdsX</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Zamora, Genesis</creator><creator>Wang, Frederick</creator><creator>Sun, Chung-Ho</creator><creator>Trinidad, Anthony</creator><creator>Kwon, Young Jik</creator><creator>Cho, Soo Kyung</creator><creator>Berg, Kristian</creator><creator>Madsen, Steen J</creator><creator>Hirschberg, Henry</creator><general>Society of Photo-Optical Instrumentation Engineers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>L7M</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20141001</creationdate><title>Photochemical internalization-mediated nonviral gene transfection: polyamine core-shell nanoparticles as gene carrier</title><author>Zamora, Genesis ; 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Biomed. Opt</addtitle><date>2014-10-01</date><risdate>2014</risdate><volume>19</volume><issue>10</issue><spage>105009</spage><epage>105009</epage><pages>105009-105009</pages><issn>1083-3668</issn><eissn>1560-2281</eissn><abstract>The overall objective of the research was to investigate the utility of photochemical internalization (PCI) for the enhanced nonviral transfection of genes into glioma cells. The PCI-mediated introduction of the tumor suppressor gene phosphatase and tensin homolog (PTEN) or the cytosine deaminase (CD) pro-drug activating gene into U87 or U251 glioma cell monolayers and multicell tumor spheroids were evaluated. In the study reported here, polyamine-DNA gene polyplexes were encapsulated in a nanoparticle (NP) with an acid degradable polyketal outer shell. These NP synthetically mimic the roles of viral capsid and envelope, which transport and release the gene, respectively. The effects of PCI-mediated suppressor and suicide genes transfection efficiency employing either "naked" polyplex cores alone or as NP-shelled cores were compared. PCI was performed with the photosensitizer AlPcS2a and λ=670-nm laser irradiance. The results clearly demonstrated that the PCI can enhance the delivery of both the PTEN or CD genes in human glioma cell monolayers and multicell tumor spheroids. The transfection efficiency, as measured by cell survival and inhibition of spheroid growth, was found to be significantly greater at suboptimal light and DNA levels for shelled NPs compared with polyamine-DNA polyplexes alone.</abstract><cop>United States</cop><pub>Society of Photo-Optical Instrumentation Engineers</pub><pmid>25341069</pmid><doi>10.1117/1.JBO.19.10.105009</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Cell Line, Tumor Cell Proliferation - drug effects Cell Proliferation - genetics Cell Survival - drug effects Cell Survival - genetics Cytosine Deaminase - genetics Cytosine Deaminase - metabolism Cytosine Deaminase - pharmacology Degradation Drug Carriers - chemistry Drug Carriers - pharmacology Drug Carriers - toxicity Genes Genetic Therapy Humans Irradiance Monolayers Nanoparticles Nanoparticles - chemistry Nanoparticles - toxicity Photochemical Photochemotherapy Photosensitizing Agents - chemistry Photosensitizing Agents - pharmacology Photosensitizing Agents - toxicity Polyamines - chemistry Polyamines - pharmacology Polyamines - toxicity PTEN Phosphohydrolase - genetics PTEN Phosphohydrolase - metabolism PTEN Phosphohydrolase - pharmacology Research Papers: General Spheroids Spheroids, Cellular Suppressors Transfection - methods Tumors |
| title | Photochemical internalization-mediated nonviral gene transfection: polyamine core-shell nanoparticles as gene carrier |
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