A phosphatase resistant substrate for the assay of protein kinase C in crude tissue extracts

Protein kinase C (PKC) is routinely assayed, after it is partially purified over DEAE-cellulose chromatography to eliminate any interfering protein kinases and phosphatases, by measuring the transfer of γ-phosphate of [γ-32P]ATP to Hl histone. Recently, it has been shown that a synthetic peptide, co...

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Veröffentlicht in:	Biochemical and biophysical research communications 1991-10, Vol.180 (2), p.694-701
Hauptverfasser:	Farrar, Young Jo K., Vanaman, Thomas C., Slevin, John T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - pharmacology Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chromatography, DEAE-Cellulose Enzymes and enzyme inhibitors Ethers, Cyclic - pharmacology Fundamental and applied biological sciences. Psychology Hippocampus - enzymology Kinetics Male Molecular Sequence Data myelin basic protein Myelin Basic Protein - chemical synthesis Myelin Basic Protein - metabolism Okadaic Acid Oligopeptides - chemical synthesis Phosphoprotein Phosphatases - metabolism Protein Kinase C - isolation & purification Protein Kinase C - metabolism Rats Rats, Inbred Strains Substrate Specificity Transferases
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