Deoxynucleotide pools and DNA synthesis in resting and PHA-stimulated human lymphocytes treated with mutagens
In resting and PHA-stimulated PBL treated with uv light or MMS we measured the sizes of the dTTP and dATP pools and the variation of ATP content taken as an indicator of cytotoxicity. The effects on DNA synthesis were examined by measuring DNA repair (unscheduled DNA synthesis) in resting PBL and th...
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| Veröffentlicht in: | Experimental cell research 1992-04, Vol.199 (2), p.349-354 |
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| container_title | Experimental cell research |
| container_volume | 199 |
| creator | Ferraro, P. Bianchi, V. Biasin, M.R. Celotti, L. |
| description | In resting and PHA-stimulated PBL treated with uv light or MMS we measured the sizes of the dTTP and dATP pools and the variation of ATP content taken as an indicator of cytotoxicity. The effects on DNA synthesis were examined by measuring DNA repair (unscheduled DNA synthesis) in resting PBL and the inhibition of DNA replication in stimulated PBL. While both treatments affected DNA synthesis, only MMS perturbed dNTP pools and decreased the intracellular concentration of ATP. All the effects were more evident in cycling than in resting lymphocytes. |
| doi_str_mv | 10.1016/0014-4827(92)90444-D |
| format | Article |
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Psychology ; Humans ; Lymphocytes - drug effects ; Lymphocytes - metabolism ; Lymphocytes - radiation effects ; methyl methane sulfonate ; Methyl Methanesulfonate - toxicity ; Molecular and cellular biology ; Molecular genetics ; Mutagenesis. 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The effects on DNA synthesis were examined by measuring DNA repair (unscheduled DNA synthesis) in resting PBL and the inhibition of DNA replication in stimulated PBL. While both treatments affected DNA synthesis, only MMS perturbed dNTP pools and decreased the intracellular concentration of ATP. All the effects were more evident in cycling than in resting lymphocytes.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>ATP</subject><subject>Biological and medical sciences</subject><subject>Cell Survival - drug effects</subject><subject>Chromatography, High Pressure Liquid</subject><subject>deoxynucleoside triphosphates</subject><subject>Deoxyribonucleotides - metabolism</subject><subject>DNA - biosynthesis</subject><subject>DNA Repair</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - metabolism</subject><subject>Lymphocytes - radiation effects</subject><subject>methyl methane sulfonate</subject><subject>Methyl Methanesulfonate - toxicity</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutagenesis. 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| ispartof | Experimental cell research, 1992-04, Vol.199 (2), p.349-354 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosine Triphosphate - metabolism ATP Biological and medical sciences Cell Survival - drug effects Chromatography, High Pressure Liquid deoxynucleoside triphosphates Deoxyribonucleotides - metabolism DNA - biosynthesis DNA Repair Fundamental and applied biological sciences. Psychology Humans Lymphocytes - drug effects Lymphocytes - metabolism Lymphocytes - radiation effects methyl methane sulfonate Methyl Methanesulfonate - toxicity Molecular and cellular biology Molecular genetics Mutagenesis. Repair Phytohemagglutinins U.V. radiation |
| title | Deoxynucleotide pools and DNA synthesis in resting and PHA-stimulated human lymphocytes treated with mutagens |
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