Nuclease-Functionalized Poly(Styrene‑b‑isobutylene‑b‑styrene) Surface with Anti-Infection and Tissue Integration Bifunctions
Hydrophobic thermoplastic elastomers, e.g., poly(styrene-b-isobutylene-b-styrene) (SIBS), have found various in vivo biomedical applications. It has long been recognized that biomaterials can be adversely affected by bacterial contamination and clinical infection. However, inhibiting bacterial colo...
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| Veröffentlicht in: | ACS applied materials & interfaces 2014-10, Vol.6 (20), p.18078-18086 |
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| creator | Yuan, Shuaishuai Zhao, Jie Luan, Shifang Yan, Shunjie Zheng, Wanling Yin, Jinghua |
| description | Hydrophobic thermoplastic elastomers, e.g., poly(styrene-b-isobutylene-b-styrene) (SIBS), have found various in vivo biomedical applications. It has long been recognized that biomaterials can be adversely affected by bacterial contamination and clinical infection. However, inhibiting bacterial colonization while simultaneously preserving or enhancing tissue-cell/material interactions is a great challenge. Herein, SIBS substrates were functionalized with nucleases under mild conditions, through polycarboxylate grafts as intermediate. It was demonstrated that the nuclease-modified SIBS could effectively prevent bacterial adhesion and biofilm formation. Cell adhesion assays confirmed that nuclease coatings generally had no negative effects on L929 cell adhesion, compared with the virgin SIBS reference. Therefore, the as-reported nuclease coating may present a promising approach to inhibit bacterial infection, while preserving tissue-cell integration on polymeric biomaterials. |
| doi_str_mv | 10.1021/am504955g |
| format | Article |
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Mater. Interfaces</addtitle><description>Hydrophobic thermoplastic elastomers, e.g., poly(styrene-b-isobutylene-b-styrene) (SIBS), have found various in vivo biomedical applications. It has long been recognized that biomaterials can be adversely affected by bacterial contamination and clinical infection. However, inhibiting bacterial colonization while simultaneously preserving or enhancing tissue-cell/material interactions is a great challenge. Herein, SIBS substrates were functionalized with nucleases under mild conditions, through polycarboxylate grafts as intermediate. It was demonstrated that the nuclease-modified SIBS could effectively prevent bacterial adhesion and biofilm formation. Cell adhesion assays confirmed that nuclease coatings generally had no negative effects on L929 cell adhesion, compared with the virgin SIBS reference. Therefore, the as-reported nuclease coating may present a promising approach to inhibit bacterial infection, while preserving tissue-cell integration on polymeric biomaterials.</description><subject>Adsorption</subject><subject>Animals</subject><subject>Bacterial Adhesion - drug effects</subject><subject>Bacterial Infections - drug therapy</subject><subject>Biofilms - growth & development</subject><subject>Cattle</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Count</subject><subject>Cell Line</subject><subject>Deoxyribonucleases - metabolism</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - physiology</subject><subject>Fibrinogen - metabolism</subject><subject>Fibroblasts - cytology</subject><subject>Mice</subject><subject>Photoelectron Spectroscopy</subject><subject>Ribonucleases - metabolism</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - physiology</subject><subject>Styrenes - pharmacology</subject><subject>Styrenes - therapeutic use</subject><subject>Surface Properties</subject><issn>1944-8244</issn><issn>1944-8252</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkLFOwzAQhi0EoqUw8AIoC1I7BOzEbuqxVBQqVYDU7pGTXIqrxCl2LBQmBl6AV-RJME0pC8PJp7tP38k_QucEXxEckGtRMkw5Y6sD1CWcUn8UsOBw31PaQSfGrDEehgFmx6jj1iwc0qiLPh5sWoAw4E-tSmtZKVHIN8i8p6po-ou60aDg6_0zcSVNldi6Kf4mpt0PvIXVuUjBe5X1szdWtfRnKoetzxMq85bSGAveTNWw0mI7vpH57qI5RUe5KAyc7d4eWk5vl5N7f_54N5uM574ICat9GrlvhpyTIcky4CFmWUTwCHhOIAIQacg4FokQlBKWBVEKfMRIOOKEchplYQ_1W-1GVy8WTB2X0qRQFEJBZU3svCyiThs5dNCiqa6M0ZDHGy1LoZuY4Pgn83ifuWMvdlqblJDtyd-QHXDZAiI18bqy2mVs_hF9A6e8jew</recordid><startdate>20141022</startdate><enddate>20141022</enddate><creator>Yuan, Shuaishuai</creator><creator>Zhao, Jie</creator><creator>Luan, Shifang</creator><creator>Yan, Shunjie</creator><creator>Zheng, Wanling</creator><creator>Yin, Jinghua</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20141022</creationdate><title>Nuclease-Functionalized Poly(Styrene‑b‑isobutylene‑b‑styrene) Surface with Anti-Infection and Tissue Integration Bifunctions</title><author>Yuan, Shuaishuai ; Zhao, Jie ; Luan, Shifang ; Yan, Shunjie ; Zheng, Wanling ; Yin, Jinghua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a315t-47504399161dde9305d7108e9f1e7eeac3590abaa4415d27ce985138914947d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adsorption</topic><topic>Animals</topic><topic>Bacterial Adhesion - drug effects</topic><topic>Bacterial Infections - drug therapy</topic><topic>Biofilms - growth & development</topic><topic>Cattle</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Count</topic><topic>Cell Line</topic><topic>Deoxyribonucleases - metabolism</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - physiology</topic><topic>Fibrinogen - metabolism</topic><topic>Fibroblasts - cytology</topic><topic>Mice</topic><topic>Photoelectron Spectroscopy</topic><topic>Ribonucleases - metabolism</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - physiology</topic><topic>Styrenes - pharmacology</topic><topic>Styrenes - therapeutic use</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Shuaishuai</creatorcontrib><creatorcontrib>Zhao, Jie</creatorcontrib><creatorcontrib>Luan, Shifang</creatorcontrib><creatorcontrib>Yan, Shunjie</creatorcontrib><creatorcontrib>Zheng, Wanling</creatorcontrib><creatorcontrib>Yin, Jinghua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS applied materials & interfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Shuaishuai</au><au>Zhao, Jie</au><au>Luan, Shifang</au><au>Yan, Shunjie</au><au>Zheng, Wanling</au><au>Yin, Jinghua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclease-Functionalized Poly(Styrene‑b‑isobutylene‑b‑styrene) Surface with Anti-Infection and Tissue Integration Bifunctions</atitle><jtitle>ACS applied materials & interfaces</jtitle><addtitle>ACS Appl. Mater. Interfaces</addtitle><date>2014-10-22</date><risdate>2014</risdate><volume>6</volume><issue>20</issue><spage>18078</spage><epage>18086</epage><pages>18078-18086</pages><issn>1944-8244</issn><eissn>1944-8252</eissn><abstract>Hydrophobic thermoplastic elastomers, e.g., poly(styrene-b-isobutylene-b-styrene) (SIBS), have found various in vivo biomedical applications. It has long been recognized that biomaterials can be adversely affected by bacterial contamination and clinical infection. However, inhibiting bacterial colonization while simultaneously preserving or enhancing tissue-cell/material interactions is a great challenge. Herein, SIBS substrates were functionalized with nucleases under mild conditions, through polycarboxylate grafts as intermediate. It was demonstrated that the nuclease-modified SIBS could effectively prevent bacterial adhesion and biofilm formation. Cell adhesion assays confirmed that nuclease coatings generally had no negative effects on L929 cell adhesion, compared with the virgin SIBS reference. Therefore, the as-reported nuclease coating may present a promising approach to inhibit bacterial infection, while preserving tissue-cell integration on polymeric biomaterials.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>25253647</pmid><doi>10.1021/am504955g</doi><tpages>9</tpages></addata></record> |
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| subjects | Adsorption Animals Bacterial Adhesion - drug effects Bacterial Infections - drug therapy Biofilms - growth & development Cattle Cell Adhesion - drug effects Cell Count Cell Line Deoxyribonucleases - metabolism Escherichia coli - drug effects Escherichia coli - physiology Fibrinogen - metabolism Fibroblasts - cytology Mice Photoelectron Spectroscopy Ribonucleases - metabolism Spectroscopy, Fourier Transform Infrared Staphylococcus aureus - drug effects Staphylococcus aureus - physiology Styrenes - pharmacology Styrenes - therapeutic use Surface Properties |
| title | Nuclease-Functionalized Poly(Styrene‑b‑isobutylene‑b‑styrene) Surface with Anti-Infection and Tissue Integration Bifunctions |
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