Paraoxon block of chloride conductance in cell R2 of Aplysia californica
In the presence of paraoxon, the amplitudes of chloride currents activated by acetylcholine (ACh) or gamma-aminobutyric acid (GABA) were reduced in cell R2 of Aplysia californica. IC 50 values were 12 and 9.7 μM for ACh and GABA responses, respectively. Paraoxon did not affect resting membrane poten...
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Veröffentlicht in: | Brain research bulletin 1992-03, Vol.28 (3), p.473-477 |
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creator | Filbert, Margaret G. Apland, James P. Petrali, John P. Adler, Michael |
description | In the presence of paraoxon, the amplitudes of chloride currents activated by acetylcholine (ACh) or gamma-aminobutyric acid (GABA) were reduced in cell R2 of
Aplysia californica. IC
50 values were 12 and 9.7 μM for ACh and GABA responses, respectively. Paraoxon did not affect resting membrane potential, input resistance, or chloride reversal potential. Both the slopes and maxima of ACh and GABA concentration-response curves were reduced by paraoxon, suggesting that paraoxon antagonism of these responses is not competitive. The antagonism of ACh and GABA responses by paraoxon was not related to inhibition of acetylcholinesterase. |
doi_str_mv | 10.1016/0361-9230(92)90049-4 |
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Aplysia californica. IC
50 values were 12 and 9.7 μM for ACh and GABA responses, respectively. Paraoxon did not affect resting membrane potential, input resistance, or chloride reversal potential. Both the slopes and maxima of ACh and GABA concentration-response curves were reduced by paraoxon, suggesting that paraoxon antagonism of these responses is not competitive. The antagonism of ACh and GABA responses by paraoxon was not related to inhibition of acetylcholinesterase.</description><identifier>ISSN: 0361-9230</identifier><identifier>EISSN: 1873-2747</identifier><identifier>DOI: 10.1016/0361-9230(92)90049-4</identifier><identifier>PMID: 1375524</identifier><identifier>CODEN: BRBUDU</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Acetylcholine ; Acetylcholine - pharmacology ; Acetylcholinesterase ; Acetylcholinesterase - metabolism ; Animal, plant and microbial ecology ; Animals ; Aplysia - metabolism ; Aplysia californica ; Applied ecology ; Biological and medical sciences ; Cell R2 ; Cells, Cultured ; Chloride channels ; Chlorides - metabolism ; Ecotoxicology, biological effects of pollution ; Effects of pollution and side effects of pesticides on protozoa and invertebrates ; Electrophysiology ; Fundamental and applied biological sciences. Psychology ; Gamma-aminobutyric acid ; gamma-Aminobutyric Acid - pharmacology ; Ganglia - drug effects ; Ganglia - metabolism ; Histocytochemistry ; Ion Channels - drug effects ; Ion Channels - metabolism ; Membrane Potentials - drug effects ; Paraoxon ; Paraoxon - pharmacology ; Voltage clamp</subject><ispartof>Brain research bulletin, 1992-03, Vol.28 (3), p.473-477</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-629825fd7f4efce7b06a98481c746fb146b53abcb07deda6ffbc69bba09a64483</citedby><cites>FETCH-LOGICAL-c417t-629825fd7f4efce7b06a98481c746fb146b53abcb07deda6ffbc69bba09a64483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0361-9230(92)90049-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5198877$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1375524$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Filbert, Margaret G.</creatorcontrib><creatorcontrib>Apland, James P.</creatorcontrib><creatorcontrib>Petrali, John P.</creatorcontrib><creatorcontrib>Adler, Michael</creatorcontrib><title>Paraoxon block of chloride conductance in cell R2 of Aplysia californica</title><title>Brain research bulletin</title><addtitle>Brain Res Bull</addtitle><description>In the presence of paraoxon, the amplitudes of chloride currents activated by acetylcholine (ACh) or gamma-aminobutyric acid (GABA) were reduced in cell R2 of
Aplysia californica. IC
50 values were 12 and 9.7 μM for ACh and GABA responses, respectively. Paraoxon did not affect resting membrane potential, input resistance, or chloride reversal potential. Both the slopes and maxima of ACh and GABA concentration-response curves were reduced by paraoxon, suggesting that paraoxon antagonism of these responses is not competitive. The antagonism of ACh and GABA responses by paraoxon was not related to inhibition of acetylcholinesterase.</description><subject>Acetylcholine</subject><subject>Acetylcholine - pharmacology</subject><subject>Acetylcholinesterase</subject><subject>Acetylcholinesterase - metabolism</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Aplysia - metabolism</subject><subject>Aplysia californica</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>Cell R2</subject><subject>Cells, Cultured</subject><subject>Chloride channels</subject><subject>Chlorides - metabolism</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Effects of pollution and side effects of pesticides on protozoa and invertebrates</subject><subject>Electrophysiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gamma-aminobutyric acid</subject><subject>gamma-Aminobutyric Acid - pharmacology</subject><subject>Ganglia - drug effects</subject><subject>Ganglia - metabolism</subject><subject>Histocytochemistry</subject><subject>Ion Channels - drug effects</subject><subject>Ion Channels - metabolism</subject><subject>Membrane Potentials - drug effects</subject><subject>Paraoxon</subject><subject>Paraoxon - pharmacology</subject><subject>Voltage clamp</subject><issn>0361-9230</issn><issn>1873-2747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotVb_gcIeRPSwmuxmk81FKEWtUFBEzyGfGN1uarIr9t-btUVvXmYO87zDzAPAMYKXCCJyBUuCclaU8JwVFwxCzHK8A8aopmVeUEx3wfgX2QcHMb5BCEldkREYoZJWVYHHYP4ogvBfvs1k49V75m2mXhsfnDaZ8q3uVSdaZTLXZso0TfZUDMh01ayjE5kSjbM-tE6JQ7BnRRPN0bZPwMvtzfNsni8e7u5n00WuMKJdTgpWF5XV1GJjlaESEsFqXCNFMbESYSKrUkglIdVGC2KtVIRJKSATBOO6nICzzd5V8B-9iR1fujicJlrj-8gRQRUtCEog3oAq-BiDsXwV3FKENUeQDwL5YIcPdlLhPwI5TrGT7f5eLo3-C22Mpfnpdi5iet-GpMfFX6xCrK4pTdj1BjPJxaczgUflTDKpXTCq49q7_-_4BtY1i-U</recordid><startdate>19920301</startdate><enddate>19920301</enddate><creator>Filbert, Margaret G.</creator><creator>Apland, James P.</creator><creator>Petrali, John P.</creator><creator>Adler, Michael</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>19920301</creationdate><title>Paraoxon block of chloride conductance in cell R2 of Aplysia californica</title><author>Filbert, Margaret G. ; Apland, James P. ; Petrali, John P. ; Adler, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-629825fd7f4efce7b06a98481c746fb146b53abcb07deda6ffbc69bba09a64483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Acetylcholine</topic><topic>Acetylcholine - pharmacology</topic><topic>Acetylcholinesterase</topic><topic>Acetylcholinesterase - metabolism</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Aplysia - metabolism</topic><topic>Aplysia californica</topic><topic>Applied ecology</topic><topic>Biological and medical sciences</topic><topic>Cell R2</topic><topic>Cells, Cultured</topic><topic>Chloride channels</topic><topic>Chlorides - metabolism</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Effects of pollution and side effects of pesticides on protozoa and invertebrates</topic><topic>Electrophysiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gamma-aminobutyric acid</topic><topic>gamma-Aminobutyric Acid - pharmacology</topic><topic>Ganglia - drug effects</topic><topic>Ganglia - metabolism</topic><topic>Histocytochemistry</topic><topic>Ion Channels - drug effects</topic><topic>Ion Channels - metabolism</topic><topic>Membrane Potentials - drug effects</topic><topic>Paraoxon</topic><topic>Paraoxon - pharmacology</topic><topic>Voltage clamp</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Filbert, Margaret G.</creatorcontrib><creatorcontrib>Apland, James P.</creatorcontrib><creatorcontrib>Petrali, John P.</creatorcontrib><creatorcontrib>Adler, Michael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Brain research bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Filbert, Margaret G.</au><au>Apland, James P.</au><au>Petrali, John P.</au><au>Adler, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Paraoxon block of chloride conductance in cell R2 of Aplysia californica</atitle><jtitle>Brain research bulletin</jtitle><addtitle>Brain Res Bull</addtitle><date>1992-03-01</date><risdate>1992</risdate><volume>28</volume><issue>3</issue><spage>473</spage><epage>477</epage><pages>473-477</pages><issn>0361-9230</issn><eissn>1873-2747</eissn><coden>BRBUDU</coden><abstract>In the presence of paraoxon, the amplitudes of chloride currents activated by acetylcholine (ACh) or gamma-aminobutyric acid (GABA) were reduced in cell R2 of
Aplysia californica. IC
50 values were 12 and 9.7 μM for ACh and GABA responses, respectively. Paraoxon did not affect resting membrane potential, input resistance, or chloride reversal potential. Both the slopes and maxima of ACh and GABA concentration-response curves were reduced by paraoxon, suggesting that paraoxon antagonism of these responses is not competitive. The antagonism of ACh and GABA responses by paraoxon was not related to inhibition of acetylcholinesterase.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>1375524</pmid><doi>10.1016/0361-9230(92)90049-4</doi><tpages>5</tpages></addata></record> |
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subjects | Acetylcholine Acetylcholine - pharmacology Acetylcholinesterase Acetylcholinesterase - metabolism Animal, plant and microbial ecology Animals Aplysia - metabolism Aplysia californica Applied ecology Biological and medical sciences Cell R2 Cells, Cultured Chloride channels Chlorides - metabolism Ecotoxicology, biological effects of pollution Effects of pollution and side effects of pesticides on protozoa and invertebrates Electrophysiology Fundamental and applied biological sciences. Psychology Gamma-aminobutyric acid gamma-Aminobutyric Acid - pharmacology Ganglia - drug effects Ganglia - metabolism Histocytochemistry Ion Channels - drug effects Ion Channels - metabolism Membrane Potentials - drug effects Paraoxon Paraoxon - pharmacology Voltage clamp |
title | Paraoxon block of chloride conductance in cell R2 of Aplysia californica |
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