Paraoxon block of chloride conductance in cell R2 of Aplysia californica

In the presence of paraoxon, the amplitudes of chloride currents activated by acetylcholine (ACh) or gamma-aminobutyric acid (GABA) were reduced in cell R2 of Aplysia californica. IC 50 values were 12 and 9.7 μM for ACh and GABA responses, respectively. Paraoxon did not affect resting membrane poten...

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Veröffentlicht in:Brain research bulletin 1992-03, Vol.28 (3), p.473-477
Hauptverfasser: Filbert, Margaret G., Apland, James P., Petrali, John P., Adler, Michael
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container_title Brain research bulletin
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creator Filbert, Margaret G.
Apland, James P.
Petrali, John P.
Adler, Michael
description In the presence of paraoxon, the amplitudes of chloride currents activated by acetylcholine (ACh) or gamma-aminobutyric acid (GABA) were reduced in cell R2 of Aplysia californica. IC 50 values were 12 and 9.7 μM for ACh and GABA responses, respectively. Paraoxon did not affect resting membrane potential, input resistance, or chloride reversal potential. Both the slopes and maxima of ACh and GABA concentration-response curves were reduced by paraoxon, suggesting that paraoxon antagonism of these responses is not competitive. The antagonism of ACh and GABA responses by paraoxon was not related to inhibition of acetylcholinesterase.
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subjects Acetylcholine
Acetylcholine - pharmacology
Acetylcholinesterase
Acetylcholinesterase - metabolism
Animal, plant and microbial ecology
Animals
Aplysia - metabolism
Aplysia californica
Applied ecology
Biological and medical sciences
Cell R2
Cells, Cultured
Chloride channels
Chlorides - metabolism
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on protozoa and invertebrates
Electrophysiology
Fundamental and applied biological sciences. Psychology
Gamma-aminobutyric acid
gamma-Aminobutyric Acid - pharmacology
Ganglia - drug effects
Ganglia - metabolism
Histocytochemistry
Ion Channels - drug effects
Ion Channels - metabolism
Membrane Potentials - drug effects
Paraoxon
Paraoxon - pharmacology
Voltage clamp
title Paraoxon block of chloride conductance in cell R2 of Aplysia californica
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