Potential- and acetylcholine-activated ionic currents of pheochromocytoma PC12 cells during incubation with nerve growth factor
Pheochromocytoma PC12 cells incubated with and without nerve growth factor were investigated using the patch-clamp technique in the whole-cell recording mode, and the concentration clamp method in the rat. On the fourth day of incubation with nerve growth factor, sodium potential-activated ionic cur...
Gespeichert in:
| Veröffentlicht in: | Neuroscience 1992, Vol.46 (4), p.925-930 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 930 |
|---|---|
| container_issue | 4 |
| container_start_page | 925 |
| container_title | Neuroscience |
| container_volume | 46 |
| creator | Baev, K.V. Beresovskii, V.K. Kalunov, V.N. Luschitskaya, N.I. Rusin, K.I. Vilner, B.Ya Zavadskaya, T.V. |
| description | Pheochromocytoma PC12 cells incubated with and without nerve growth factor were investigated using the patch-clamp technique in the whole-cell recording mode, and the concentration clamp method in the rat. On the fourth day of incubation with nerve growth factor, sodium potential-activated ionic currents appeared in the membranes of the most morphologically differentiated cells. At the same period a three-fold increase of acetylcholine-activated current density, compared with the cells incubated without nerve growth factor, was observed.
Thus, the qualitative and quantitative changes in membrane properties can be a result of metabolic reorganization in PC12 cells induced by nerve growth factor and accompanied by morphological differentiation according to neuronal phenotype. |
| doi_str_mv | 10.1016/0306-4522(92)90194-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16152999</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0306452292901947</els_id><sourcerecordid>16152999</sourcerecordid><originalsourceid>FETCH-LOGICAL-c303t-4eddf6d75e0a7501640d41d3f5d5256eb28cbcdc1ddcaa084eb23327791d33f3</originalsourceid><addsrcrecordid>eNp9kMFrHCEUxqW0pJu0_0ELnkpzmFZHHXcuhbAkbSGQHHIX9_kma5nRrTob9tR_PQ4b2ltFkOf7fZ--j5APnH3hjHdfmWBdI1Xbfu7by57xXjb6FVnxtRaNVlK-Jqu_yFtynvMvVpeS4oyccaH5WnUr8uc-FgzF27GhNjhqActxhF0cfcDGQvEHW9BRH4MHCnNKlc40DnS_wwi7FKcIxxInS-83vKWA45ipm5MPj9QHmLe2VC198mVHA6YD0scUn2oxVPOY3pE3gx0zvn85L8jDzfXD5kdze_f95-bqtgHBRGkkOjd0TitkVqs6vWROcicG5VSrOty2a9iCA-4cWMvWst4I0WrdV0gM4oJ8OtnuU_w9Yy5m8nn5qw0Y52x4x1Xb930F5QmEFHNOOJh98pNNR8OZWWI3S6ZmydT0dS-xG11lH1_85-2E7p_olHPtfzv1sc548JhMBo8B0PmEUIyL_v8PPAPoOZRp</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16152999</pqid></control><display><type>article</type><title>Potential- and acetylcholine-activated ionic currents of pheochromocytoma PC12 cells during incubation with nerve growth factor</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Baev, K.V. ; Beresovskii, V.K. ; Kalunov, V.N. ; Luschitskaya, N.I. ; Rusin, K.I. ; Vilner, B.Ya ; Zavadskaya, T.V.</creator><creatorcontrib>Baev, K.V. ; Beresovskii, V.K. ; Kalunov, V.N. ; Luschitskaya, N.I. ; Rusin, K.I. ; Vilner, B.Ya ; Zavadskaya, T.V.</creatorcontrib><description>Pheochromocytoma PC12 cells incubated with and without nerve growth factor were investigated using the patch-clamp technique in the whole-cell recording mode, and the concentration clamp method in the rat. On the fourth day of incubation with nerve growth factor, sodium potential-activated ionic currents appeared in the membranes of the most morphologically differentiated cells. At the same period a three-fold increase of acetylcholine-activated current density, compared with the cells incubated without nerve growth factor, was observed.
Thus, the qualitative and quantitative changes in membrane properties can be a result of metabolic reorganization in PC12 cells induced by nerve growth factor and accompanied by morphological differentiation according to neuronal phenotype.</description><identifier>ISSN: 0306-4522</identifier><identifier>EISSN: 1873-7544</identifier><identifier>DOI: 10.1016/0306-4522(92)90194-7</identifier><identifier>PMID: 1371856</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Acetylcholine - pharmacology ; Adrenal Gland Neoplasms ; Animals ; Dose-Response Relationship, Drug ; Evoked Potentials - drug effects ; HEPES ; Ion Channels - drug effects ; Ion Channels - physiology ; Kinetics ; N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid ; nerve growth factor ; Nerve Growth Factors - pharmacology ; NGF ; PC12 Cells ; Pheochromocytoma ; Potassium Channels - drug effects ; Potassium Channels - physiology ; Sodium Channels - drug effects ; Sodium Channels - physiology ; Tetrodotoxin - pharmacology ; Time Factors</subject><ispartof>Neuroscience, 1992, Vol.46 (4), p.925-930</ispartof><rights>1992 IBRO</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-4eddf6d75e0a7501640d41d3f5d5256eb28cbcdc1ddcaa084eb23327791d33f3</citedby><cites>FETCH-LOGICAL-c303t-4eddf6d75e0a7501640d41d3f5d5256eb28cbcdc1ddcaa084eb23327791d33f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0306-4522(92)90194-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,4021,27921,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1371856$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baev, K.V.</creatorcontrib><creatorcontrib>Beresovskii, V.K.</creatorcontrib><creatorcontrib>Kalunov, V.N.</creatorcontrib><creatorcontrib>Luschitskaya, N.I.</creatorcontrib><creatorcontrib>Rusin, K.I.</creatorcontrib><creatorcontrib>Vilner, B.Ya</creatorcontrib><creatorcontrib>Zavadskaya, T.V.</creatorcontrib><title>Potential- and acetylcholine-activated ionic currents of pheochromocytoma PC12 cells during incubation with nerve growth factor</title><title>Neuroscience</title><addtitle>Neuroscience</addtitle><description>Pheochromocytoma PC12 cells incubated with and without nerve growth factor were investigated using the patch-clamp technique in the whole-cell recording mode, and the concentration clamp method in the rat. On the fourth day of incubation with nerve growth factor, sodium potential-activated ionic currents appeared in the membranes of the most morphologically differentiated cells. At the same period a three-fold increase of acetylcholine-activated current density, compared with the cells incubated without nerve growth factor, was observed.
Thus, the qualitative and quantitative changes in membrane properties can be a result of metabolic reorganization in PC12 cells induced by nerve growth factor and accompanied by morphological differentiation according to neuronal phenotype.</description><subject>Acetylcholine - pharmacology</subject><subject>Adrenal Gland Neoplasms</subject><subject>Animals</subject><subject>Dose-Response Relationship, Drug</subject><subject>Evoked Potentials - drug effects</subject><subject>HEPES</subject><subject>Ion Channels - drug effects</subject><subject>Ion Channels - physiology</subject><subject>Kinetics</subject><subject>N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid</subject><subject>nerve growth factor</subject><subject>Nerve Growth Factors - pharmacology</subject><subject>NGF</subject><subject>PC12 Cells</subject><subject>Pheochromocytoma</subject><subject>Potassium Channels - drug effects</subject><subject>Potassium Channels - physiology</subject><subject>Sodium Channels - drug effects</subject><subject>Sodium Channels - physiology</subject><subject>Tetrodotoxin - pharmacology</subject><subject>Time Factors</subject><issn>0306-4522</issn><issn>1873-7544</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFrHCEUxqW0pJu0_0ELnkpzmFZHHXcuhbAkbSGQHHIX9_kma5nRrTob9tR_PQ4b2ltFkOf7fZ--j5APnH3hjHdfmWBdI1Xbfu7by57xXjb6FVnxtRaNVlK-Jqu_yFtynvMvVpeS4oyccaH5WnUr8uc-FgzF27GhNjhqActxhF0cfcDGQvEHW9BRH4MHCnNKlc40DnS_wwi7FKcIxxInS-83vKWA45ipm5MPj9QHmLe2VC198mVHA6YD0scUn2oxVPOY3pE3gx0zvn85L8jDzfXD5kdze_f95-bqtgHBRGkkOjd0TitkVqs6vWROcicG5VSrOty2a9iCA-4cWMvWst4I0WrdV0gM4oJ8OtnuU_w9Yy5m8nn5qw0Y52x4x1Xb930F5QmEFHNOOJh98pNNR8OZWWI3S6ZmydT0dS-xG11lH1_85-2E7p_olHPtfzv1sc548JhMBo8B0PmEUIyL_v8PPAPoOZRp</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Baev, K.V.</creator><creator>Beresovskii, V.K.</creator><creator>Kalunov, V.N.</creator><creator>Luschitskaya, N.I.</creator><creator>Rusin, K.I.</creator><creator>Vilner, B.Ya</creator><creator>Zavadskaya, T.V.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>1992</creationdate><title>Potential- and acetylcholine-activated ionic currents of pheochromocytoma PC12 cells during incubation with nerve growth factor</title><author>Baev, K.V. ; Beresovskii, V.K. ; Kalunov, V.N. ; Luschitskaya, N.I. ; Rusin, K.I. ; Vilner, B.Ya ; Zavadskaya, T.V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-4eddf6d75e0a7501640d41d3f5d5256eb28cbcdc1ddcaa084eb23327791d33f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Acetylcholine - pharmacology</topic><topic>Adrenal Gland Neoplasms</topic><topic>Animals</topic><topic>Dose-Response Relationship, Drug</topic><topic>Evoked Potentials - drug effects</topic><topic>HEPES</topic><topic>Ion Channels - drug effects</topic><topic>Ion Channels - physiology</topic><topic>Kinetics</topic><topic>N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid</topic><topic>nerve growth factor</topic><topic>Nerve Growth Factors - pharmacology</topic><topic>NGF</topic><topic>PC12 Cells</topic><topic>Pheochromocytoma</topic><topic>Potassium Channels - drug effects</topic><topic>Potassium Channels - physiology</topic><topic>Sodium Channels - drug effects</topic><topic>Sodium Channels - physiology</topic><topic>Tetrodotoxin - pharmacology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baev, K.V.</creatorcontrib><creatorcontrib>Beresovskii, V.K.</creatorcontrib><creatorcontrib>Kalunov, V.N.</creatorcontrib><creatorcontrib>Luschitskaya, N.I.</creatorcontrib><creatorcontrib>Rusin, K.I.</creatorcontrib><creatorcontrib>Vilner, B.Ya</creatorcontrib><creatorcontrib>Zavadskaya, T.V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baev, K.V.</au><au>Beresovskii, V.K.</au><au>Kalunov, V.N.</au><au>Luschitskaya, N.I.</au><au>Rusin, K.I.</au><au>Vilner, B.Ya</au><au>Zavadskaya, T.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potential- and acetylcholine-activated ionic currents of pheochromocytoma PC12 cells during incubation with nerve growth factor</atitle><jtitle>Neuroscience</jtitle><addtitle>Neuroscience</addtitle><date>1992</date><risdate>1992</risdate><volume>46</volume><issue>4</issue><spage>925</spage><epage>930</epage><pages>925-930</pages><issn>0306-4522</issn><eissn>1873-7544</eissn><abstract>Pheochromocytoma PC12 cells incubated with and without nerve growth factor were investigated using the patch-clamp technique in the whole-cell recording mode, and the concentration clamp method in the rat. On the fourth day of incubation with nerve growth factor, sodium potential-activated ionic currents appeared in the membranes of the most morphologically differentiated cells. At the same period a three-fold increase of acetylcholine-activated current density, compared with the cells incubated without nerve growth factor, was observed.
Thus, the qualitative and quantitative changes in membrane properties can be a result of metabolic reorganization in PC12 cells induced by nerve growth factor and accompanied by morphological differentiation according to neuronal phenotype.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>1371856</pmid><doi>10.1016/0306-4522(92)90194-7</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0306-4522 |
| ispartof | Neuroscience, 1992, Vol.46 (4), p.925-930 |
| issn | 0306-4522 1873-7544 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16152999 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acetylcholine - pharmacology Adrenal Gland Neoplasms Animals Dose-Response Relationship, Drug Evoked Potentials - drug effects HEPES Ion Channels - drug effects Ion Channels - physiology Kinetics N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid nerve growth factor Nerve Growth Factors - pharmacology NGF PC12 Cells Pheochromocytoma Potassium Channels - drug effects Potassium Channels - physiology Sodium Channels - drug effects Sodium Channels - physiology Tetrodotoxin - pharmacology Time Factors |
| title | Potential- and acetylcholine-activated ionic currents of pheochromocytoma PC12 cells during incubation with nerve growth factor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T18%3A42%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Potential-%20and%20acetylcholine-activated%20ionic%20currents%20of%20pheochromocytoma%20PC12%20cells%20during%20incubation%20with%20nerve%20growth%20factor&rft.jtitle=Neuroscience&rft.au=Baev,%20K.V.&rft.date=1992&rft.volume=46&rft.issue=4&rft.spage=925&rft.epage=930&rft.pages=925-930&rft.issn=0306-4522&rft.eissn=1873-7544&rft_id=info:doi/10.1016/0306-4522(92)90194-7&rft_dat=%3Cproquest_cross%3E16152999%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16152999&rft_id=info:pmid/1371856&rft_els_id=0306452292901947&rfr_iscdi=true |