Investigation of cell exudates active in carbon tetrachloride and chloroform degradation
Contamination of groundwater by chlorinated solvents such as carbon tetrachloride (CCl4) and chloroform (CHCl3) is a widespread problem. The cell exudates from the methanogen Methanosarcina thermophila are active in the degradation of CCl4 and CHCl3. This research was performed to characterize these...
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| Veröffentlicht in: | Biotechnology and bioengineering 2001-07, Vol.74 (1), p.12-17 |
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| creator | Koons, Brad W. Baeseman, Jenny L. Novak, Paige J. |
| description | Contamination of groundwater by chlorinated solvents such as carbon tetrachloride (CCl4) and chloroform (CHCl3) is a widespread problem. The cell exudates from the methanogen Methanosarcina thermophila are active in the degradation of CCl4 and CHCl3. This research was performed to characterize these exudates. Examination of the influence of pH indicated that activity was greater under alkaline conditions. Rapid CCl4 degradation occurred from 35–65°C, with first‐order degradation rate coefficients increasing as temperature increased. It was found that proteins were not responsible for CCl4 degradation. The active agents in the cell exudates were |
| doi_str_mv | 10.1002/bit.1090 |
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The cell exudates from the methanogen Methanosarcina thermophila are active in the degradation of CCl4 and CHCl3. This research was performed to characterize these exudates. Examination of the influence of pH indicated that activity was greater under alkaline conditions. Rapid CCl4 degradation occurred from 35–65°C, with first‐order degradation rate coefficients increasing as temperature increased. It was found that proteins were not responsible for CCl4 degradation. The active agents in the cell exudates were <10 kDa in size, with degradation activity present in both 1–10 kDa and <1 kDa size ranges. Upon purification of the <10 kDa size range of the cell exudates on a C18 chromatography column, 17 fractions (out of 100) degraded >50% of the added CCl4 in 8 h. These 17 fractions were pooled into three samples based on their elution time from the C18 column. One of these pooled samples contained elevated levels of cobalt, zinc, and iron, at 2, 3, and 13 times the levels measured in similarly fractionated and pooled samples of medium, respectively. The UV‐visible spectrum of this pooled sample had an absorption maximum at 560–580 nm, which is similar to the absorption maxima of heme (approximately 550 and 575 nm). The two other pooled samples contained elevated levels of zinc at 11 and 22 times the concentration measured in similarly fractionated and pooled samples of medium, respectively, and also contained very low levels of nickel, cobalt, and iron. This research suggests that the cell exudates from M. thermophila contain porphorinogen‐type molecules capable of dechlorination, possibly excreted corrinoids, hemes, and zinc‐containing molecules. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 74; 12–17, 2001.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.1090</identifier><identifier>PMID: 11353406</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Biodegradation of pollutants ; Biodegradation, Environmental ; Biological and medical sciences ; Biotechnology ; Carbon Tetrachloride - metabolism ; cell exudates ; Chloroform - metabolism ; dechlorination ; Environment and pollution ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Industrial applications and implications. Economical aspects ; methanogen ; Methanosarcina - metabolism ; Methanosarcina thermophila ; Oxygen - metabolism ; Particle Size ; porphorinogen ; porphyrin ; Temperature</subject><ispartof>Biotechnology and bioengineering, 2001-07, Vol.74 (1), p.12-17</ispartof><rights>Copyright © 2001 John Wiley & Sons, Inc.</rights><rights>2001 INIST-CNRS</rights><rights>Copyright 2001 John Wiley & Sons, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4900-ece01fac968f7e6439e1ea7164e68573bc2d884d335870529d34679deebdaa623</citedby><cites>FETCH-LOGICAL-c4900-ece01fac968f7e6439e1ea7164e68573bc2d884d335870529d34679deebdaa623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.1090$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.1090$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1117536$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11353406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koons, Brad W.</creatorcontrib><creatorcontrib>Baeseman, Jenny L.</creatorcontrib><creatorcontrib>Novak, Paige J.</creatorcontrib><title>Investigation of cell exudates active in carbon tetrachloride and chloroform degradation</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>Contamination of groundwater by chlorinated solvents such as carbon tetrachloride (CCl4) and chloroform (CHCl3) is a widespread problem. The cell exudates from the methanogen Methanosarcina thermophila are active in the degradation of CCl4 and CHCl3. This research was performed to characterize these exudates. Examination of the influence of pH indicated that activity was greater under alkaline conditions. Rapid CCl4 degradation occurred from 35–65°C, with first‐order degradation rate coefficients increasing as temperature increased. It was found that proteins were not responsible for CCl4 degradation. The active agents in the cell exudates were <10 kDa in size, with degradation activity present in both 1–10 kDa and <1 kDa size ranges. Upon purification of the <10 kDa size range of the cell exudates on a C18 chromatography column, 17 fractions (out of 100) degraded >50% of the added CCl4 in 8 h. These 17 fractions were pooled into three samples based on their elution time from the C18 column. One of these pooled samples contained elevated levels of cobalt, zinc, and iron, at 2, 3, and 13 times the levels measured in similarly fractionated and pooled samples of medium, respectively. The UV‐visible spectrum of this pooled sample had an absorption maximum at 560–580 nm, which is similar to the absorption maxima of heme (approximately 550 and 575 nm). The two other pooled samples contained elevated levels of zinc at 11 and 22 times the concentration measured in similarly fractionated and pooled samples of medium, respectively, and also contained very low levels of nickel, cobalt, and iron. This research suggests that the cell exudates from M. thermophila contain porphorinogen‐type molecules capable of dechlorination, possibly excreted corrinoids, hemes, and zinc‐containing molecules. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 74; 12–17, 2001.</description><subject>Biodegradation of pollutants</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Carbon Tetrachloride - metabolism</subject><subject>cell exudates</subject><subject>Chloroform - metabolism</subject><subject>dechlorination</subject><subject>Environment and pollution</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>methanogen</subject><subject>Methanosarcina - metabolism</subject><subject>Methanosarcina thermophila</subject><subject>Oxygen - metabolism</subject><subject>Particle Size</subject><subject>porphorinogen</subject><subject>porphyrin</subject><subject>Temperature</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10MFO3DAQBmCrKipbQOoTIB-qikvAjhM7PrKowAoECIFAvVgTewJuswnYWQpvj5eNWi6cZkb6NKP5CfnG2S5nLN-r_ZAazT6RSSoqY7lmn8mEMSYzUep8nXyN8XcaVSXlF7LOuShFweSE3M66J4yDv4PB9x3tG2qxbSk-LxwMGCnYwT8h9R21EOokBhwC2Pu2D94hhc7Rt6Fv-jCnDu8CuLdVm2StgTbi1lg3yPXhz6uD4-z0_Gh2sH-a2UIzlqFFxhuwWlaNQlkIjRxBcVmgrEolapu7qiqcEGWlWJlrJwqptEOsHYDMxQb5sdr7EPrHRXrFzH1c_gAd9otouOS5znmR4M4K2tDHGLAxD8HPIbwYzswyRZNSNMsUE90edy7qObr_cIwtge8jgGihbQJ01sd3jqtSLFm2Yn99iy8f3jPT2dV4d_Q-Dvj8z0P4Y6QSqjQ3Z0dmenFyeXHLp-aXeAUwjpf0</recordid><startdate>20010705</startdate><enddate>20010705</enddate><creator>Koons, Brad W.</creator><creator>Baeseman, Jenny L.</creator><creator>Novak, Paige J.</creator><general>John Wiley & Sons, Inc</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TV</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20010705</creationdate><title>Investigation of cell exudates active in carbon tetrachloride and chloroform degradation</title><author>Koons, Brad W. ; Baeseman, Jenny L. ; Novak, Paige J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4900-ece01fac968f7e6439e1ea7164e68573bc2d884d335870529d34679deebdaa623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Biodegradation of pollutants</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Carbon Tetrachloride - metabolism</topic><topic>cell exudates</topic><topic>Chloroform - metabolism</topic><topic>dechlorination</topic><topic>Environment and pollution</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>methanogen</topic><topic>Methanosarcina - metabolism</topic><topic>Methanosarcina thermophila</topic><topic>Oxygen - metabolism</topic><topic>Particle Size</topic><topic>porphorinogen</topic><topic>porphyrin</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koons, Brad W.</creatorcontrib><creatorcontrib>Baeseman, Jenny L.</creatorcontrib><creatorcontrib>Novak, Paige J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Pollution Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koons, Brad W.</au><au>Baeseman, Jenny L.</au><au>Novak, Paige J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of cell exudates active in carbon tetrachloride and chloroform degradation</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>2001-07-05</date><risdate>2001</risdate><volume>74</volume><issue>1</issue><spage>12</spage><epage>17</epage><pages>12-17</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>Contamination of groundwater by chlorinated solvents such as carbon tetrachloride (CCl4) and chloroform (CHCl3) is a widespread problem. The cell exudates from the methanogen Methanosarcina thermophila are active in the degradation of CCl4 and CHCl3. This research was performed to characterize these exudates. Examination of the influence of pH indicated that activity was greater under alkaline conditions. Rapid CCl4 degradation occurred from 35–65°C, with first‐order degradation rate coefficients increasing as temperature increased. It was found that proteins were not responsible for CCl4 degradation. The active agents in the cell exudates were <10 kDa in size, with degradation activity present in both 1–10 kDa and <1 kDa size ranges. Upon purification of the <10 kDa size range of the cell exudates on a C18 chromatography column, 17 fractions (out of 100) degraded >50% of the added CCl4 in 8 h. These 17 fractions were pooled into three samples based on their elution time from the C18 column. One of these pooled samples contained elevated levels of cobalt, zinc, and iron, at 2, 3, and 13 times the levels measured in similarly fractionated and pooled samples of medium, respectively. The UV‐visible spectrum of this pooled sample had an absorption maximum at 560–580 nm, which is similar to the absorption maxima of heme (approximately 550 and 575 nm). The two other pooled samples contained elevated levels of zinc at 11 and 22 times the concentration measured in similarly fractionated and pooled samples of medium, respectively, and also contained very low levels of nickel, cobalt, and iron. This research suggests that the cell exudates from M. thermophila contain porphorinogen‐type molecules capable of dechlorination, possibly excreted corrinoids, hemes, and zinc‐containing molecules. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 74; 12–17, 2001.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>11353406</pmid><doi>10.1002/bit.1090</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biodegradation of pollutants Biodegradation, Environmental Biological and medical sciences Biotechnology Carbon Tetrachloride - metabolism cell exudates Chloroform - metabolism dechlorination Environment and pollution Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Industrial applications and implications. Economical aspects methanogen Methanosarcina - metabolism Methanosarcina thermophila Oxygen - metabolism Particle Size porphorinogen porphyrin Temperature |
| title | Investigation of cell exudates active in carbon tetrachloride and chloroform degradation |
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