Amino-terminal processing of actins mutagenized at the Cys super(-1) residue

We constructed mutant actins in which the cysteine was replaced with serine, asparagine, glycine aspartic acid, histidine, phenylalanine, and tyrosine and used these to determine the substrate specificity of rat liver actin N-acetylaminopeptidase in vitro. The results suggest a narrow substrate specificity for the rat liver actin N-acetylaminopeptidase. They also demonstrate that the adjacent residue can affect actin N-acetylaminopeptidase specificity.