Storage at low temperature differentially affects the colour and carotenoid composition of two cultivars of banana

•Carotenoid profiles of two banana cultivars were assessed during cold storage.•Five carotenoids are being reported for the first time in banana pulp.•Skin colour of the banana fruits was not affected by low temperatures.•Carotenoids levels in cv. Prata decreased with cold storage.•Banana cv. Nanicão showed increased levels of carotenoids in response to stress. Different storage conditions can induce changes in the colour and carotenoid profiles and levels in some fruits. The goal of this work was to evaluate the influence of low temperature storage on the colour and carotenoid synthesis in two banana cultivars: Prata and Nanicão. For this purpose, the carotenoids from the banana pulp were determined by HPLC–DAD–MS/MS, and the colour of the banana skin was determined by a colorimeter method. Ten carotenoids were identified, of which the major carotenoids were all-trans-lutein, all-trans-α-carotene and all-trans-β-carotene in both cultivars. The effect of the low temperatures was subjected to linear regression analysis. In cv. Prata, all-trans-α-carotene and all-trans-β-carotene were significantly affected by low temperature (p0.05). The accumulation of carotenoids in this group may be because the metabolic pathways using these carotenoids were affected by storage at low temperatures. The colour of the fruits was not negatively affected by the low temperatures (p>0.05).