The use of commercial serum replacements in media for the in vitro replication of nuclear polyhedrosis virus
Two commercially available fetal bovine serum replacements, previously used in media for the culture of vertebrate and insect cells, were evaluated in comparison with fetal bovine serum (FBS) for suitability as medium supplements for the production of the Autographa californica nuclear polyhedrosis...
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| Veröffentlicht in: | Journal of invertebrate pathology 1991, Vol.58 (3), p.297-304 |
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| creator | Vaughn, J.L. Fan, F. Dougherty, E.M. Adams, J.R. Guzo, D. McClintock, J.T. |
| description | Two commercially available fetal bovine serum replacements, previously used in media for the culture of vertebrate and insect cells, were evaluated in comparison with fetal bovine serum (FBS) for suitability as medium supplements for the production of the
Autographa californica nuclear polyhedrosis virus in fall armyworm cell cultures. The overall patterns of viral protein synthesis were similar in infected cells in each of the media supplements, but some differences were noted in quantities of individual proteins produced and in the temporal regulation of the synthesis. The release of extracellular budded virions (ECV) in all media began at about the same time and the rate of release was similar. However, the final concentration of ECV was consistently lower in medium containing either CPSR-1 or CPSR-3 than that in FBS supplemented medium. The number of occlusion bodies (OB) per cell was also less in the CPSR-1 medium. In the CPSR-3 medium the number OBs per cell produced was not significantly different from those in the FBS medium. The LC
50 value of the OBs bioassayed in neonate
Spodoptera frugiperda produced in the CPRS-3 medium was significantly lower than that of OBs from FBS medium but the LC
70 and LC
90 values were not. Abnormal OBs, that is OBs that contained few or no occluded virions, were observed in cells in media with all three supplements. |
| doi_str_mv | 10.1016/0022-2011(91)90174-O |
| format | Article |
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Autographa californica nuclear polyhedrosis virus in fall armyworm cell cultures. The overall patterns of viral protein synthesis were similar in infected cells in each of the media supplements, but some differences were noted in quantities of individual proteins produced and in the temporal regulation of the synthesis. The release of extracellular budded virions (ECV) in all media began at about the same time and the rate of release was similar. However, the final concentration of ECV was consistently lower in medium containing either CPSR-1 or CPSR-3 than that in FBS supplemented medium. The number of occlusion bodies (OB) per cell was also less in the CPSR-1 medium. In the CPSR-3 medium the number OBs per cell produced was not significantly different from those in the FBS medium. The LC
50 value of the OBs bioassayed in neonate
Spodoptera frugiperda produced in the CPRS-3 medium was significantly lower than that of OBs from FBS medium but the LC
70 and LC
90 values were not. Abnormal OBs, that is OBs that contained few or no occluded virions, were observed in cells in media with all three supplements.</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1016/0022-2011(91)90174-O</identifier><identifier>CODEN: JIVPAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>autographa californica ; Autographa californica NPV ; auxiliaire de lutte biologique ; baculoviridae ; Biological and medical sciences ; biological control ; biological control organisms ; cell culture ; cultivo de celulas ; cultivo in vitro ; culture de cellule ; culture in vitro ; culture media ; experimentation en laboratoire ; experimentos en laboratorio ; Fundamental and applied biological sciences. Psychology ; in vitro culture ; Insecta ; insecticidas ; insecticide ; insecticides ; invertebrate viruses ; Invertebrates ; laboratory experiments ; large volume production ; media (culture) ; medio de cultivo ; methode ; methods ; metodos ; microbial pesticides ; milieu de culture ; nuclear polyhedrosis virus ; organismos para control biologico ; pathogenicity ; Pathology ; pesticide microbien ; plaguicidas microbianos ; poder patogeno ; pouvoir pathogene ; protein synthesis ; proteinas ; proteine ; proteins ; serum-free media ; sintesis de proteinas ; Spodoptera frugiperda ; synthese proteique ; virus ; virus de invertebrados ; virus des invertebres ; viruses</subject><ispartof>Journal of invertebrate pathology, 1991, Vol.58 (3), p.297-304</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c302t-913c11461277473a25e7a2698d1802d191e426d1174aa63407b15ab162d10ba23</citedby><cites>FETCH-LOGICAL-c302t-913c11461277473a25e7a2698d1802d191e426d1174aa63407b15ab162d10ba23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-2011(91)90174-O$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,4022,27922,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5302226$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Vaughn, J.L.</creatorcontrib><creatorcontrib>Fan, F.</creatorcontrib><creatorcontrib>Dougherty, E.M.</creatorcontrib><creatorcontrib>Adams, J.R.</creatorcontrib><creatorcontrib>Guzo, D.</creatorcontrib><creatorcontrib>McClintock, J.T.</creatorcontrib><creatorcontrib>Insect Biocontrol Laboratory, ARS, USDA, Beltsville, MD</creatorcontrib><title>The use of commercial serum replacements in media for the in vitro replication of nuclear polyhedrosis virus</title><title>Journal of invertebrate pathology</title><description>Two commercially available fetal bovine serum replacements, previously used in media for the culture of vertebrate and insect cells, were evaluated in comparison with fetal bovine serum (FBS) for suitability as medium supplements for the production of the
Autographa californica nuclear polyhedrosis virus in fall armyworm cell cultures. The overall patterns of viral protein synthesis were similar in infected cells in each of the media supplements, but some differences were noted in quantities of individual proteins produced and in the temporal regulation of the synthesis. The release of extracellular budded virions (ECV) in all media began at about the same time and the rate of release was similar. However, the final concentration of ECV was consistently lower in medium containing either CPSR-1 or CPSR-3 than that in FBS supplemented medium. The number of occlusion bodies (OB) per cell was also less in the CPSR-1 medium. In the CPSR-3 medium the number OBs per cell produced was not significantly different from those in the FBS medium. The LC
50 value of the OBs bioassayed in neonate
Spodoptera frugiperda produced in the CPRS-3 medium was significantly lower than that of OBs from FBS medium but the LC
70 and LC
90 values were not. Abnormal OBs, that is OBs that contained few or no occluded virions, were observed in cells in media with all three supplements.</description><subject>autographa californica</subject><subject>Autographa californica NPV</subject><subject>auxiliaire de lutte biologique</subject><subject>baculoviridae</subject><subject>Biological and medical sciences</subject><subject>biological control</subject><subject>biological control organisms</subject><subject>cell culture</subject><subject>cultivo de celulas</subject><subject>cultivo in vitro</subject><subject>culture de cellule</subject><subject>culture in vitro</subject><subject>culture media</subject><subject>experimentation en laboratoire</subject><subject>experimentos en laboratorio</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>in vitro culture</subject><subject>Insecta</subject><subject>insecticidas</subject><subject>insecticide</subject><subject>insecticides</subject><subject>invertebrate viruses</subject><subject>Invertebrates</subject><subject>laboratory experiments</subject><subject>large volume production</subject><subject>media (culture)</subject><subject>medio de cultivo</subject><subject>methode</subject><subject>methods</subject><subject>metodos</subject><subject>microbial pesticides</subject><subject>milieu de culture</subject><subject>nuclear polyhedrosis virus</subject><subject>organismos para control biologico</subject><subject>pathogenicity</subject><subject>Pathology</subject><subject>pesticide microbien</subject><subject>plaguicidas microbianos</subject><subject>poder patogeno</subject><subject>pouvoir pathogene</subject><subject>protein synthesis</subject><subject>proteinas</subject><subject>proteine</subject><subject>proteins</subject><subject>serum-free media</subject><subject>sintesis de proteinas</subject><subject>Spodoptera frugiperda</subject><subject>synthese proteique</subject><subject>virus</subject><subject>virus de invertebrados</subject><subject>virus des invertebres</subject><subject>viruses</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNp9kFFrFTEQhYMoeK3-A5E8iNiH1Ux2N7t5EUqxKhTug-1zmJudtZHs5prsFvrvnfWWPgqBhMl3DnOOEG9BfQIF5rNSWldaAXy0cG4VdE21fyZ2oKypVK_a52L3hLwUr0r5rfjVGrsT8eaO5FpIplH6NE2UfcAoC-V1kpmOET1NNC9FhllONASUY8pyYRUP7sOS0z8seFxCmjebefWRMMtjig93NORUQmEyr-W1eDFiLPTm8T4Tt1dfby6_V9f7bz8uL64rXyu9VBZqD9AY0F3XdDXqljrUxvYD9EoPYIEabQbgnIimblR3gBYPYPhPHVDXZ-LDyfeY05-VyuKmUDzFiDOltTgwAL21NYPNCfS8Zck0umMOE-YHB8pt1bqtN7f15iyfrVq3Z9n7R38sHuOYcfahPGlbTqG1YezdCRsxOfyVGbn9CdZqpQzn6hn4cgKIy7gPlF3xgWbPNWfyixtS-P8ifwH2ppVx</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>Vaughn, J.L.</creator><creator>Fan, F.</creator><creator>Dougherty, E.M.</creator><creator>Adams, J.R.</creator><creator>Guzo, D.</creator><creator>McClintock, J.T.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>1991</creationdate><title>The use of commercial serum replacements in media for the in vitro replication of nuclear polyhedrosis virus</title><author>Vaughn, J.L. ; Fan, F. ; Dougherty, E.M. ; Adams, J.R. ; Guzo, D. ; McClintock, J.T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c302t-913c11461277473a25e7a2698d1802d191e426d1174aa63407b15ab162d10ba23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>autographa californica</topic><topic>Autographa californica NPV</topic><topic>auxiliaire de lutte biologique</topic><topic>baculoviridae</topic><topic>Biological and medical sciences</topic><topic>biological control</topic><topic>biological control organisms</topic><topic>cell culture</topic><topic>cultivo de celulas</topic><topic>cultivo in vitro</topic><topic>culture de cellule</topic><topic>culture in vitro</topic><topic>culture media</topic><topic>experimentation en laboratoire</topic><topic>experimentos en laboratorio</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>in vitro culture</topic><topic>Insecta</topic><topic>insecticidas</topic><topic>insecticide</topic><topic>insecticides</topic><topic>invertebrate viruses</topic><topic>Invertebrates</topic><topic>laboratory experiments</topic><topic>large volume production</topic><topic>media (culture)</topic><topic>medio de cultivo</topic><topic>methode</topic><topic>methods</topic><topic>metodos</topic><topic>microbial pesticides</topic><topic>milieu de culture</topic><topic>nuclear polyhedrosis virus</topic><topic>organismos para control biologico</topic><topic>pathogenicity</topic><topic>Pathology</topic><topic>pesticide microbien</topic><topic>plaguicidas microbianos</topic><topic>poder patogeno</topic><topic>pouvoir pathogene</topic><topic>protein synthesis</topic><topic>proteinas</topic><topic>proteine</topic><topic>proteins</topic><topic>serum-free media</topic><topic>sintesis de proteinas</topic><topic>Spodoptera frugiperda</topic><topic>synthese proteique</topic><topic>virus</topic><topic>virus de invertebrados</topic><topic>virus des invertebres</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vaughn, J.L.</creatorcontrib><creatorcontrib>Fan, F.</creatorcontrib><creatorcontrib>Dougherty, E.M.</creatorcontrib><creatorcontrib>Adams, J.R.</creatorcontrib><creatorcontrib>Guzo, D.</creatorcontrib><creatorcontrib>McClintock, J.T.</creatorcontrib><creatorcontrib>Insect Biocontrol Laboratory, ARS, USDA, Beltsville, MD</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vaughn, J.L.</au><au>Fan, F.</au><au>Dougherty, E.M.</au><au>Adams, J.R.</au><au>Guzo, D.</au><au>McClintock, J.T.</au><aucorp>Insect Biocontrol Laboratory, ARS, USDA, Beltsville, MD</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The use of commercial serum replacements in media for the in vitro replication of nuclear polyhedrosis virus</atitle><jtitle>Journal of invertebrate pathology</jtitle><date>1991</date><risdate>1991</risdate><volume>58</volume><issue>3</issue><spage>297</spage><epage>304</epage><pages>297-304</pages><issn>0022-2011</issn><eissn>1096-0805</eissn><coden>JIVPAZ</coden><abstract>Two commercially available fetal bovine serum replacements, previously used in media for the culture of vertebrate and insect cells, were evaluated in comparison with fetal bovine serum (FBS) for suitability as medium supplements for the production of the
Autographa californica nuclear polyhedrosis virus in fall armyworm cell cultures. The overall patterns of viral protein synthesis were similar in infected cells in each of the media supplements, but some differences were noted in quantities of individual proteins produced and in the temporal regulation of the synthesis. The release of extracellular budded virions (ECV) in all media began at about the same time and the rate of release was similar. However, the final concentration of ECV was consistently lower in medium containing either CPSR-1 or CPSR-3 than that in FBS supplemented medium. The number of occlusion bodies (OB) per cell was also less in the CPSR-1 medium. In the CPSR-3 medium the number OBs per cell produced was not significantly different from those in the FBS medium. The LC
50 value of the OBs bioassayed in neonate
Spodoptera frugiperda produced in the CPRS-3 medium was significantly lower than that of OBs from FBS medium but the LC
70 and LC
90 values were not. Abnormal OBs, that is OBs that contained few or no occluded virions, were observed in cells in media with all three supplements.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><doi>10.1016/0022-2011(91)90174-O</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of invertebrate pathology, 1991, Vol.58 (3), p.297-304 |
| issn | 0022-2011 1096-0805 |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | autographa californica Autographa californica NPV auxiliaire de lutte biologique baculoviridae Biological and medical sciences biological control biological control organisms cell culture cultivo de celulas cultivo in vitro culture de cellule culture in vitro culture media experimentation en laboratoire experimentos en laboratorio Fundamental and applied biological sciences. Psychology in vitro culture Insecta insecticidas insecticide insecticides invertebrate viruses Invertebrates laboratory experiments large volume production media (culture) medio de cultivo methode methods metodos microbial pesticides milieu de culture nuclear polyhedrosis virus organismos para control biologico pathogenicity Pathology pesticide microbien plaguicidas microbianos poder patogeno pouvoir pathogene protein synthesis proteinas proteine proteins serum-free media sintesis de proteinas Spodoptera frugiperda synthese proteique virus virus de invertebrados virus des invertebres viruses |
| title | The use of commercial serum replacements in media for the in vitro replication of nuclear polyhedrosis virus |
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