Electroporation of pKK1 silver-resistance plasmid from Pseudomonas stutzeri AG259 into Pseudomonas putida CYM318
Plasmid pKK1 (49.4 Mda), which encodes for Ag super(+) resistance, was isolated from Pseudomonas stutzeri) AG259 (pKK1) and introduced into Pseudomonas putida CYM318 by high-voltage electroporation. Upon acquiring pKK1, P. putida CYM318 became resistant to AgNO sub(3). This demonstrated that electro...
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| Veröffentlicht in: | Current microbiology 1990-08, Vol.21 (2), p.103-107 |
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| container_title | Current microbiology |
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| creator | TREVORS, J. T STARODUB, M. E |
| description | Plasmid pKK1 (49.4 Mda), which encodes for Ag super(+) resistance, was isolated from Pseudomonas stutzeri) AG259 (pKK1) and introduced into Pseudomonas putida CYM318 by high-voltage electroporation. Upon acquiring pKK1, P. putida CYM318 became resistant to AgNO sub(3). This demonstrated that electroporation is a useful method to introduce a nonconjugative metal-resistance plasmid into a bacterial strain and to conduct further comparative research on Ag accumulation. |
| doi_str_mv | 10.1007/BF02091827 |
| format | Article |
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T ; STARODUB, M. E</creator><creatorcontrib>TREVORS, J. T ; STARODUB, M. E</creatorcontrib><description>Plasmid pKK1 (49.4 Mda), which encodes for Ag super(+) resistance, was isolated from Pseudomonas stutzeri) AG259 (pKK1) and introduced into Pseudomonas putida CYM318 by high-voltage electroporation. Upon acquiring pKK1, P. putida CYM318 became resistant to AgNO sub(3). This demonstrated that electroporation is a useful method to introduce a nonconjugative metal-resistance plasmid into a bacterial strain and to conduct further comparative research on Ag accumulation.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/BF02091827</identifier><identifier>CODEN: CUMIDD</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; cations ; electroporation ; Fundamental and applied biological sciences. 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E</creatorcontrib><title>Electroporation of pKK1 silver-resistance plasmid from Pseudomonas stutzeri AG259 into Pseudomonas putida CYM318</title><title>Current microbiology</title><description>Plasmid pKK1 (49.4 Mda), which encodes for Ag super(+) resistance, was isolated from Pseudomonas stutzeri) AG259 (pKK1) and introduced into Pseudomonas putida CYM318 by high-voltage electroporation. Upon acquiring pKK1, P. putida CYM318 became resistant to AgNO sub(3). This demonstrated that electroporation is a useful method to introduce a nonconjugative metal-resistance plasmid into a bacterial strain and to conduct further comparative research on Ag accumulation.</description><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>cations</subject><subject>electroporation</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Microbiology</topic><topic>plasmids</topic><topic>Pseudomonas stutzeri</topic><topic>silver</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TREVORS, J. T</creatorcontrib><creatorcontrib>STARODUB, M. E</creatorcontrib><collection>Pascal-Francis</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TREVORS, J. T</au><au>STARODUB, M. E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electroporation of pKK1 silver-resistance plasmid from Pseudomonas stutzeri AG259 into Pseudomonas putida CYM318</atitle><jtitle>Current microbiology</jtitle><date>1990-08-01</date><risdate>1990</risdate><volume>21</volume><issue>2</issue><spage>103</spage><epage>107</epage><pages>103-107</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>Plasmid pKK1 (49.4 Mda), which encodes for Ag super(+) resistance, was isolated from Pseudomonas stutzeri) AG259 (pKK1) and introduced into Pseudomonas putida CYM318 by high-voltage electroporation. Upon acquiring pKK1, P. putida CYM318 became resistant to AgNO sub(3). This demonstrated that electroporation is a useful method to introduce a nonconjugative metal-resistance plasmid into a bacterial strain and to conduct further comparative research on Ag accumulation.</abstract><cop>New York, NY</cop><pub>Springer</pub><doi>10.1007/BF02091827</doi><tpages>5</tpages></addata></record> |
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| ispartof | Current microbiology, 1990-08, Vol.21 (2), p.103-107 |
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| language | eng |
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| subjects | Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences cations electroporation Fundamental and applied biological sciences. Psychology Microbiology plasmids Pseudomonas stutzeri silver |
| title | Electroporation of pKK1 silver-resistance plasmid from Pseudomonas stutzeri AG259 into Pseudomonas putida CYM318 |
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