Electroporation of pKK1 silver-resistance plasmid from Pseudomonas stutzeri AG259 into Pseudomonas putida CYM318

Electroporation of pKK1 silver-resistance plasmid from Pseudomonas stutzeri AG259 into Pseudomonas putida CYM318

Plasmid pKK1 (49.4 Mda), which encodes for Ag super(+) resistance, was isolated from Pseudomonas stutzeri) AG259 (pKK1) and introduced into Pseudomonas putida CYM318 by high-voltage electroporation. Upon acquiring pKK1, P. putida CYM318 became resistant to AgNO sub(3). This demonstrated that electro...

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Veröffentlicht in:Current microbiology 1990-08, Vol.21 (2), p.103-107
Hauptverfasser: TREVORS, J. T, STARODUB, M. E
Format: Artikel
Sprache:eng
Schlagworte:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
cations
electroporation
Fundamental and applied biological sciences. Psychology
Microbiology
plasmids
Pseudomonas stutzeri
silver
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Zusammenfassung:Plasmid pKK1 (49.4 Mda), which encodes for Ag super(+) resistance, was isolated from Pseudomonas stutzeri) AG259 (pKK1) and introduced into Pseudomonas putida CYM318 by high-voltage electroporation. Upon acquiring pKK1, P. putida CYM318 became resistant to AgNO sub(3). This demonstrated that electroporation is a useful method to introduce a nonconjugative metal-resistance plasmid into a bacterial strain and to conduct further comparative research on Ag accumulation.
ISSN:0343-8651
1432-0991
DOI:10.1007/BF02091827