Middle-down hybrid chromatography/tandem mass spectrometry workflow for characterization of combinatorial post-translational modifications in histones

We present an integrated middle‐down proteomics platform for sensitive mapping and quantification of coexisting PTMs in large polypeptides (5–7 kDa). We combined an RP trap column with subsequent weak cation exchange–hydrophilic interaction LC interfaced directly to high mass accuracy ESI MS/MS usin...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Proteomics (Weinheim) 2014-10, Vol.14 (19), p.2200-2211
Hauptverfasser:	Sidoli, Simone, Schwämmle, Veit, Ruminowicz, Chrystian, Hansen, Thomas A., Wu, Xudong, Helin, Kristian, Jensen, Ole N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Line Chromatography, High Pressure Liquid Computational Biology - methods Embryonic Stem Cells Histones Histones - analysis Histones - chemistry Histones - metabolism Hybrid chromatography Methylation Mice Middle-down proteomics Post-translational modifications Protein Processing, Post-Translational - physiology Spectrometry, Mass, Electrospray Ionization Stem cells Tandem Mass Spectrometry - methods Technology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!