Characterization of Ascidian Plasma Growth Factors Promoting the Proliferation of Mouse Thymocytes

Characterization of Ascidian Plasma Growth Factors Promoting the Proliferation of Mouse Thymocytes

The plasma of a solitaly ascidian, Halocynthia roretzi, stimulates the proliferation of mouse thymocytes in the presence of a mitogen, phytohemagglutinin P (PHA-P). Eight factors capable of stimulating the proliferation of mouse thymocytes were isolated from H. roretzi plasma by procedures including...

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Veröffentlicht in:Zoological Science 1997-04, Vol.14 (2), p.271-276
Hauptverfasser: Iizuka, Junko, Azumi, Kaoru, Yokosawa, Hideyoshi
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Halocynthia roretzi
Marine
Original s
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creator Iizuka, Junko
Azumi, Kaoru
Yokosawa, Hideyoshi
description The plasma of a solitaly ascidian, Halocynthia roretzi, stimulates the proliferation of mouse thymocytes in the presence of a mitogen, phytohemagglutinin P (PHA-P). Eight factors capable of stimulating the proliferation of mouse thymocytes were isolated from H. roretzi plasma by procedures including hydrophobic chromatography, gel filtration, and anion-exchange chromatography. Four active fractions with molecular weights of 100–110 K, 75–90 K, 10–30 K, and 5–6 K were first separated by gel filtration. Each of these fractions was then separated into two fractions with different ionic properties by anion-exchange chromatography. The eight factors thus obtained have different heat stabilities, heparin-binding abilities, and PHA-P sensitivities.
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subjects Halocynthia roretzi
Marine
Original s
title Characterization of Ascidian Plasma Growth Factors Promoting the Proliferation of Mouse Thymocytes
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