Oxygen uptake kinetics of Pseudomonas chlororaphis grown in glucose- or glutamate-limited continuous cultures
Oxygen uptake and glucose and glutamate oxidation kinetics of the heterotrophic bacterium Pseudomonas chlororaphis grown in glucose- or glutamate-limited cultures under oxygen-saturating or oxygen-limiting conditions were determined. Km values for oxygen were 1.4= 5.6 micromolars. Only in the case o...
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| Veröffentlicht in: | Archives of microbiology 1997-06, Vol.167 (6), p.392-395 |
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| container_end_page | 395 |
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| container_title | Archives of microbiology |
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| creator | Bodelier, P.L.E Laanbroek, H.J |
| description | Oxygen uptake and glucose and glutamate oxidation kinetics of the heterotrophic bacterium Pseudomonas chlororaphis grown in glucose- or glutamate-limited cultures under oxygen-saturating or oxygen-limiting conditions were determined. Km values for oxygen were 1.4= 5.6 micromolars. Only in the case of glucose were significantly lower Km values and enhanced specific oxygen affinity (Vmax/Km) per cell found under oxygen-limiting conditions. Both Km and specific affinity values for glucose and glutamate oxidation were apparently affected by oxygen concentration, although a statistically significant enhancement of the oxidation kinetics was found only for glutamate. The kinetic data found for P. chlororaphis support the conclusion that the outcome of competition for oxygen with Nitrosomonas europaea in the rhizosphere of oxygen-releasing macrophytes will primarily be determined by oxidation kinetics of the electron donor instead of the oxygen uptake kinetics of the respective organisms. |
| doi_str_mv | 10.1007/s002030050460 |
| format | Article |
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Km values for oxygen were 1.4= 5.6 micromolars. Only in the case of glucose were significantly lower Km values and enhanced specific oxygen affinity (Vmax/Km) per cell found under oxygen-limiting conditions. Both Km and specific affinity values for glucose and glutamate oxidation were apparently affected by oxygen concentration, although a statistically significant enhancement of the oxidation kinetics was found only for glutamate. The kinetic data found for P. chlororaphis support the conclusion that the outcome of competition for oxygen with Nitrosomonas europaea in the rhizosphere of oxygen-releasing macrophytes will primarily be determined by oxidation kinetics of the electron donor instead of the oxygen uptake kinetics of the respective organisms.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s002030050460</identifier><identifier>CODEN: AMICCW</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Bacteriology ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Metabolism. 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Km values for oxygen were 1.4= 5.6 micromolars. Only in the case of glucose were significantly lower Km values and enhanced specific oxygen affinity (Vmax/Km) per cell found under oxygen-limiting conditions. Both Km and specific affinity values for glucose and glutamate oxidation were apparently affected by oxygen concentration, although a statistically significant enhancement of the oxidation kinetics was found only for glutamate. The kinetic data found for P. chlororaphis support the conclusion that the outcome of competition for oxygen with Nitrosomonas europaea in the rhizosphere of oxygen-releasing macrophytes will primarily be determined by oxidation kinetics of the electron donor instead of the oxygen uptake kinetics of the respective organisms.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Nitrosomonas europaea</subject><subject>Pseudomonas chlororaphis</subject><subject>soil biology</subject><subject>soil science</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNpVkc1r3DAQxUVJoJuPY8_VofTmZCTLknUsS5sEFhJIArkZrTzeVdeWtpJMmv8-ChsCOc0w_N5jeI-QbwwuGIC6TAAcaoAGhIQvZMFEzStQ_OmILMqdV62u66_kJKW_AIy3bbsg0-3_lw16Ou-z2SHdOY_Z2UTDQO8Szn2YgjeJ2u0YYohmv3WJbmJ49tR5uhlnGxJWNMS3PZvJZKxGN7mMPbXBZ-fnMBf5POY5Yjojx4MZE56_z1Py-Of3w_K6Wt1e3Sx_rSoruMgVN1rLZm0bDoB9j4aptVYa22aNyiilkFthlUTBB4k9amUbiaD5UGvbr219Sn4efPcx_Jsx5W5yyeI4Go_ln45JBkoIUcDqANoYUoo4dPvoJhNfOgbdW6jdp1AL_-Pd2CRrxiEab136EHEpdQuqYN8P2GBCZzaxII_3HFgpoZUKSg2vZ5KCtw</recordid><startdate>19970601</startdate><enddate>19970601</enddate><creator>Bodelier, P.L.E</creator><creator>Laanbroek, H.J</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>19970601</creationdate><title>Oxygen uptake kinetics of Pseudomonas chlororaphis grown in glucose- or glutamate-limited continuous cultures</title><author>Bodelier, P.L.E ; Laanbroek, H.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-2a9965bc5200eddea17b979e85be7a777e2c4c76e42f6ede97c56e092f39cdbc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Nitrosomonas europaea</topic><topic>Pseudomonas chlororaphis</topic><topic>soil biology</topic><topic>soil science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bodelier, P.L.E</creatorcontrib><creatorcontrib>Laanbroek, H.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bodelier, P.L.E</au><au>Laanbroek, H.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oxygen uptake kinetics of Pseudomonas chlororaphis grown in glucose- or glutamate-limited continuous cultures</atitle><jtitle>Archives of microbiology</jtitle><date>1997-06-01</date><risdate>1997</risdate><volume>167</volume><issue>6</issue><spage>392</spage><epage>395</epage><pages>392-395</pages><issn>0302-8933</issn><eissn>1432-072X</eissn><coden>AMICCW</coden><abstract>Oxygen uptake and glucose and glutamate oxidation kinetics of the heterotrophic bacterium Pseudomonas chlororaphis grown in glucose- or glutamate-limited cultures under oxygen-saturating or oxygen-limiting conditions were determined. Km values for oxygen were 1.4= 5.6 micromolars. Only in the case of glucose were significantly lower Km values and enhanced specific oxygen affinity (Vmax/Km) per cell found under oxygen-limiting conditions. Both Km and specific affinity values for glucose and glutamate oxidation were apparently affected by oxygen concentration, although a statistically significant enhancement of the oxidation kinetics was found only for glutamate. The kinetic data found for P. chlororaphis support the conclusion that the outcome of competition for oxygen with Nitrosomonas europaea in the rhizosphere of oxygen-releasing macrophytes will primarily be determined by oxidation kinetics of the electron donor instead of the oxygen uptake kinetics of the respective organisms.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s002030050460</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| subjects | Bacteriology Biological and medical sciences Fundamental and applied biological sciences. Psychology Metabolism. Enzymes Microbiology Nitrosomonas europaea Pseudomonas chlororaphis soil biology soil science |
| title | Oxygen uptake kinetics of Pseudomonas chlororaphis grown in glucose- or glutamate-limited continuous cultures |
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