Biosynthesis of pregnane derivatives in somatic embryos of Digitalis lanata
Activities of enzymes and concentrations of intermediates involved in the biosynthesis of 5β-pregnane-3β-ol,20-one were determined in somatic embryos of Digitalis lanata at different developmental stages. Activities of the enzymes cholesterol monooxygenase (side chain cleaving) (SCCE) Δ 5-3β- hydrox...
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| Veröffentlicht in: | Phytochemistry (Oxford) 1997-10, Vol.46 (3), p.507-513 |
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| creator | Lindemann, Peter Luckner, Martin |
| description | Activities of enzymes and concentrations of intermediates involved in the biosynthesis of 5β-pregnane-3β-ol,20-one were determined in somatic embryos of
Digitalis lanata at different developmental stages.
Activities of the enzymes cholesterol monooxygenase (side chain cleaving) (SCCE)
Δ
5-3β-
hydroxysteroiddehydrogenase
Δ
5-Δ
4-
ketosteroid
isomerase, progesterone 5β-reductase, 3β-hydroxysteroid 5β-oxidoreductase, 3α-hydroxysteroid 5β-oxidoreductase, progesterone 5α-reductase and 3β-hydroxysteroid 5α-oxidoreductase which are involved in biosynthesis and transformation of pregnane derivatives were determined in different developmental stages of somatic embryos of
Digitalis lanata. All enzymes were found to be present in proembryogenic masses (PEMs) as well as in globular and bipolar embryos. Most SCCE activity was found in the mitochondria fraction. Cholesterol, sitosterol and stigmasterol, precursors of the pregnanes, occurred in somatic embryos in amounts of about 1 μg mg
−1 protein. Pregnenolone was found in traces only (about 20 ng mg
−1 protein). Feeding of progesterone caused an increase of the contents of 5α-pand 5β-pregnandione, progesterone, 5β-pregnane-3α-ol,20-one and 5α-pregnane-3β-ol,-20-one. In contrast, administration of cholesterol caused a small increase of pregnenolone only. These results indicate that the rate limiting step in pregnane (and probably in cardenolide biosynthesis) is compartmentation of CSSE in the mitochondria of the somatic embryos of
D. lanata. |
| doi_str_mv | 10.1016/S0031-9422(97)00315-4 |
| format | Article |
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Digitalis lanata at different developmental stages.
Activities of the enzymes cholesterol monooxygenase (side chain cleaving) (SCCE)
Δ
5-3β-
hydroxysteroiddehydrogenase
Δ
5-Δ
4-
ketosteroid
isomerase, progesterone 5β-reductase, 3β-hydroxysteroid 5β-oxidoreductase, 3α-hydroxysteroid 5β-oxidoreductase, progesterone 5α-reductase and 3β-hydroxysteroid 5α-oxidoreductase which are involved in biosynthesis and transformation of pregnane derivatives were determined in different developmental stages of somatic embryos of
Digitalis lanata. All enzymes were found to be present in proembryogenic masses (PEMs) as well as in globular and bipolar embryos. Most SCCE activity was found in the mitochondria fraction. Cholesterol, sitosterol and stigmasterol, precursors of the pregnanes, occurred in somatic embryos in amounts of about 1 μg mg
−1 protein. Pregnenolone was found in traces only (about 20 ng mg
−1 protein). Feeding of progesterone caused an increase of the contents of 5α-pand 5β-pregnandione, progesterone, 5β-pregnane-3α-ol,20-one and 5α-pregnane-3β-ol,-20-one. In contrast, administration of cholesterol caused a small increase of pregnenolone only. These results indicate that the rate limiting step in pregnane (and probably in cardenolide biosynthesis) is compartmentation of CSSE in the mitochondria of the somatic embryos of
D. lanata.</description><identifier>ISSN: 0031-9422</identifier><identifier>EISSN: 1873-3700</identifier><identifier>DOI: 10.1016/S0031-9422(97)00315-4</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Biotechnology ; Digitalis lanata ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. Technologies ; Miscellaneous ; Plant cells and fungal cells ; Plant physiology and development ; Tissue cultures, protoplasts</subject><ispartof>Phytochemistry (Oxford), 1997-10, Vol.46 (3), p.507-513</ispartof><rights>1997</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-5e817b6e31cdcd8709b44bf460a17f48f4c1a60f40260bd8ef239c185fffd5d63</citedby><cites>FETCH-LOGICAL-c367t-5e817b6e31cdcd8709b44bf460a17f48f4c1a60f40260bd8ef239c185fffd5d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0031-9422(97)00315-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27928,27929,45999</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2810673$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Lindemann, Peter</creatorcontrib><creatorcontrib>Luckner, Martin</creatorcontrib><title>Biosynthesis of pregnane derivatives in somatic embryos of Digitalis lanata</title><title>Phytochemistry (Oxford)</title><description>Activities of enzymes and concentrations of intermediates involved in the biosynthesis of 5β-pregnane-3β-ol,20-one were determined in somatic embryos of
Digitalis lanata at different developmental stages.
Activities of the enzymes cholesterol monooxygenase (side chain cleaving) (SCCE)
Δ
5-3β-
hydroxysteroiddehydrogenase
Δ
5-Δ
4-
ketosteroid
isomerase, progesterone 5β-reductase, 3β-hydroxysteroid 5β-oxidoreductase, 3α-hydroxysteroid 5β-oxidoreductase, progesterone 5α-reductase and 3β-hydroxysteroid 5α-oxidoreductase which are involved in biosynthesis and transformation of pregnane derivatives were determined in different developmental stages of somatic embryos of
Digitalis lanata. All enzymes were found to be present in proembryogenic masses (PEMs) as well as in globular and bipolar embryos. Most SCCE activity was found in the mitochondria fraction. Cholesterol, sitosterol and stigmasterol, precursors of the pregnanes, occurred in somatic embryos in amounts of about 1 μg mg
−1 protein. Pregnenolone was found in traces only (about 20 ng mg
−1 protein). Feeding of progesterone caused an increase of the contents of 5α-pand 5β-pregnandione, progesterone, 5β-pregnane-3α-ol,20-one and 5α-pregnane-3β-ol,-20-one. In contrast, administration of cholesterol caused a small increase of pregnenolone only. These results indicate that the rate limiting step in pregnane (and probably in cardenolide biosynthesis) is compartmentation of CSSE in the mitochondria of the somatic embryos of
D. lanata.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Digitalis lanata</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>Miscellaneous</subject><subject>Plant cells and fungal cells</subject><subject>Plant physiology and development</subject><subject>Tissue cultures, protoplasts</subject><issn>0031-9422</issn><issn>1873-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkM1OwzAQhC0EEqXwCEg5IASHwDp27OSEoPyKShyAs-XY62KUJsVOK_XtSdqKK6fVSN_M7g4hpxSuKFBx_Q7AaFryLLso5eUg8pTvkREtJEuZBNgnoz_kkBzF-A0AeS7EiLze-Taum-4Lo49J65JFwFmjG0wsBr_SnV9hTHyTxHbeC5PgvArrdoPe-5nvdN37at3oTh-TA6friCe7OSafjw8fk-d0-vb0MrmdpoYJ2aU5FlRWAhk11thCQllxXjkuQFPpeOG4oVqA45AJqGyBLmOloUXunLO5FWxMzre5i9D-LDF2au6jwbq_AttlVFRQyEvGejDfgia0MQZ0ahH8XIe1oqCG6tSmOjX0okqpNtUp3vvOdgt0NLp2QTfGxz9zVlAQcoi_2WLYP7vyGFQ0HhuD1gc0nbKt_2fRL6RMgzY</recordid><startdate>19971001</startdate><enddate>19971001</enddate><creator>Lindemann, Peter</creator><creator>Luckner, Martin</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19971001</creationdate><title>Biosynthesis of pregnane derivatives in somatic embryos of Digitalis lanata</title><author>Lindemann, Peter ; Luckner, Martin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-5e817b6e31cdcd8709b44bf460a17f48f4c1a60f40260bd8ef239c185fffd5d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Digitalis lanata</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Miscellaneous</topic><topic>Plant cells and fungal cells</topic><topic>Plant physiology and development</topic><topic>Tissue cultures, protoplasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lindemann, Peter</creatorcontrib><creatorcontrib>Luckner, Martin</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Phytochemistry (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lindemann, Peter</au><au>Luckner, Martin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biosynthesis of pregnane derivatives in somatic embryos of Digitalis lanata</atitle><jtitle>Phytochemistry (Oxford)</jtitle><date>1997-10-01</date><risdate>1997</risdate><volume>46</volume><issue>3</issue><spage>507</spage><epage>513</epage><pages>507-513</pages><issn>0031-9422</issn><eissn>1873-3700</eissn><abstract>Activities of enzymes and concentrations of intermediates involved in the biosynthesis of 5β-pregnane-3β-ol,20-one were determined in somatic embryos of
Digitalis lanata at different developmental stages.
Activities of the enzymes cholesterol monooxygenase (side chain cleaving) (SCCE)
Δ
5-3β-
hydroxysteroiddehydrogenase
Δ
5-Δ
4-
ketosteroid
isomerase, progesterone 5β-reductase, 3β-hydroxysteroid 5β-oxidoreductase, 3α-hydroxysteroid 5β-oxidoreductase, progesterone 5α-reductase and 3β-hydroxysteroid 5α-oxidoreductase which are involved in biosynthesis and transformation of pregnane derivatives were determined in different developmental stages of somatic embryos of
Digitalis lanata. All enzymes were found to be present in proembryogenic masses (PEMs) as well as in globular and bipolar embryos. Most SCCE activity was found in the mitochondria fraction. Cholesterol, sitosterol and stigmasterol, precursors of the pregnanes, occurred in somatic embryos in amounts of about 1 μg mg
−1 protein. Pregnenolone was found in traces only (about 20 ng mg
−1 protein). Feeding of progesterone caused an increase of the contents of 5α-pand 5β-pregnandione, progesterone, 5β-pregnane-3α-ol,20-one and 5α-pregnane-3β-ol,-20-one. In contrast, administration of cholesterol caused a small increase of pregnenolone only. These results indicate that the rate limiting step in pregnane (and probably in cardenolide biosynthesis) is compartmentation of CSSE in the mitochondria of the somatic embryos of
D. lanata.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><doi>10.1016/S0031-9422(97)00315-4</doi><tpages>7</tpages></addata></record> |
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| ispartof | Phytochemistry (Oxford), 1997-10, Vol.46 (3), p.507-513 |
| issn | 0031-9422 1873-3700 |
| language | eng |
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| subjects | Biological and medical sciences Biotechnology Digitalis lanata Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Miscellaneous Plant cells and fungal cells Plant physiology and development Tissue cultures, protoplasts |
| title | Biosynthesis of pregnane derivatives in somatic embryos of Digitalis lanata |
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