Possible involvement of protein kinase C in mediating gastrin-induced response in rat colonic epithelium
We examined the potential role of protein kinase C in signal transduction induced by gastrin's stimulation of rat colonic epithelium. Protein synthesis ([ 35S]methionine incorporation into protein) and enzyme activity (decrease in the cytosolic activity) were measured following epithelial stimu...
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| Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 1991-09, Vol.12 (5), p.925-927 |
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| creator | Yassin, Rihab R. Murthy, S.N.S. |
| description | We examined the potential role of protein kinase C in signal transduction induced by gastrin's stimulation of rat colonic epithelium. Protein synthesis ([
35S]methionine incorporation into protein) and enzyme activity (decrease in the cytosolic activity) were measured following epithelial stimulation with gastrin. Gastrin (10 nM) increased [
35S]methionine incorporation into protein to 265% above maintenance level. The effect of gastrin was comparable to the stimulation induced by phorbol 12-myristate, 13-acetate (PMA), a strong activator of protein kinase C. The increase in protein synthesis induced by gastrin was totally abolished by 1-(5-isoquinolinyl)-2-methylpiperazine, an inhibitor of protein kinase C activity. Gastrin also decreased the cytosolic activity of the enzyme, an index of its activation and subsequent translocation to other cellular compartments. Therefore, we conclude that gastrin may be acting through a protein kinase C mechanism. |
| doi_str_mv | 10.1016/0196-9781(91)90039-R |
| format | Article |
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35S]methionine incorporation into protein) and enzyme activity (decrease in the cytosolic activity) were measured following epithelial stimulation with gastrin. Gastrin (10 nM) increased [
35S]methionine incorporation into protein to 265% above maintenance level. The effect of gastrin was comparable to the stimulation induced by phorbol 12-myristate, 13-acetate (PMA), a strong activator of protein kinase C. The increase in protein synthesis induced by gastrin was totally abolished by 1-(5-isoquinolinyl)-2-methylpiperazine, an inhibitor of protein kinase C activity. Gastrin also decreased the cytosolic activity of the enzyme, an index of its activation and subsequent translocation to other cellular compartments. Therefore, we conclude that gastrin may be acting through a protein kinase C mechanism.</description><identifier>ISSN: 0196-9781</identifier><identifier>EISSN: 1873-5169</identifier><identifier>DOI: 10.1016/0196-9781(91)90039-R</identifier><identifier>PMID: 1800956</identifier><identifier>CODEN: PPTDD5</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine ; Analysis of Variance ; Animals ; Biological and medical sciences ; colon ; Colon - drug effects ; Colon - enzymology ; Colon - metabolism ; Epithelium - drug effects ; Epithelium - enzymology ; Epithelium - metabolism ; Fundamental and applied biological sciences. Psychology ; Gastrin ; Gastrins - pharmacology ; In Vitro Techniques ; Intestine. Mesentery ; Isoquinolines - pharmacology ; Kinetics ; Male ; Methionine - metabolism ; Piperazines - pharmacology ; PMA ; Protein Biosynthesis ; Protein kinase C ; Protein Kinase C - antagonists & inhibitors ; Protein Kinase C - metabolism ; Protein synthesis ; Rats ; Rats, Inbred Strains ; Tetradecanoylphorbol Acetate - pharmacology ; Vertebrates: digestive system</subject><ispartof>Peptides (New York, N.Y. : 1980), 1991-09, Vol.12 (5), p.925-927</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-2fcd3f0f497908f6410a68be2cd90bc56856a0a0fd7ef83ad1a4e699724d53363</citedby><cites>FETCH-LOGICAL-c417t-2fcd3f0f497908f6410a68be2cd90bc56856a0a0fd7ef83ad1a4e699724d53363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/019697819190039R$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4995291$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1800956$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yassin, Rihab R.</creatorcontrib><creatorcontrib>Murthy, S.N.S.</creatorcontrib><title>Possible involvement of protein kinase C in mediating gastrin-induced response in rat colonic epithelium</title><title>Peptides (New York, N.Y. : 1980)</title><addtitle>Peptides</addtitle><description>We examined the potential role of protein kinase C in signal transduction induced by gastrin's stimulation of rat colonic epithelium. Protein synthesis ([
35S]methionine incorporation into protein) and enzyme activity (decrease in the cytosolic activity) were measured following epithelial stimulation with gastrin. Gastrin (10 nM) increased [
35S]methionine incorporation into protein to 265% above maintenance level. The effect of gastrin was comparable to the stimulation induced by phorbol 12-myristate, 13-acetate (PMA), a strong activator of protein kinase C. The increase in protein synthesis induced by gastrin was totally abolished by 1-(5-isoquinolinyl)-2-methylpiperazine, an inhibitor of protein kinase C activity. Gastrin also decreased the cytosolic activity of the enzyme, an index of its activation and subsequent translocation to other cellular compartments. Therefore, we conclude that gastrin may be acting through a protein kinase C mechanism.</description><subject>1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine</subject><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>colon</subject><subject>Colon - drug effects</subject><subject>Colon - enzymology</subject><subject>Colon - metabolism</subject><subject>Epithelium - drug effects</subject><subject>Epithelium - enzymology</subject><subject>Epithelium - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastrin</subject><subject>Gastrins - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Intestine. Mesentery</subject><subject>Isoquinolines - pharmacology</subject><subject>Kinetics</subject><subject>Male</subject><subject>Methionine - metabolism</subject><subject>Piperazines - pharmacology</subject><subject>PMA</subject><subject>Protein Biosynthesis</subject><subject>Protein kinase C</subject><subject>Protein Kinase C - antagonists & inhibitors</subject><subject>Protein Kinase C - metabolism</subject><subject>Protein synthesis</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Vertebrates: digestive system</subject><issn>0196-9781</issn><issn>1873-5169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQhoMo67j6DxRyENFDa2U6ne5cFmTwCxaURc8hk1R2S7vTY5Ie8N-bZob1JhTk8D71UnkYey7grQCh3oHQqtH9IF5r8UYDtLq5ecA2YujbphNKP2Sbe-Qxe5LzTwCQUg8X7EIMALpTG3b3bc6Z9iNyisd5POKEsfA58EOaC1LkvyjajHxXcz6hJ1so3vJbm0ui2FD0i0PPE-bDHPPawpMt3M3jHMlxPFC5w5GW6Sl7FOyY8dn5vWQ_Pn74vvvcXH_99GX3_rpxUvSl2Qbn2wBB6l7DEJQUYNWwx63zGvauU0OnLFgIvscwtNYLK1Fp3W-l79pWtZfs1am3fuD3grmYibLDcbQR5yUboQS0Sq-gPIEuVQUJgzkkmmz6YwSYVbBZ7ZnVntF1VsHmpq69OPcv--rj39LJaM1fnnObnR1DstFRvsek1t1Wi4pdnTCsLo6EyWRHGKtLSuiK8TP9_46_3omYtQ</recordid><startdate>19910901</startdate><enddate>19910901</enddate><creator>Yassin, Rihab R.</creator><creator>Murthy, S.N.S.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope></search><sort><creationdate>19910901</creationdate><title>Possible involvement of protein kinase C in mediating gastrin-induced response in rat colonic epithelium</title><author>Yassin, Rihab R. ; Murthy, S.N.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-2fcd3f0f497908f6410a68be2cd90bc56856a0a0fd7ef83ad1a4e699724d53363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine</topic><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>colon</topic><topic>Colon - drug effects</topic><topic>Colon - enzymology</topic><topic>Colon - metabolism</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - enzymology</topic><topic>Epithelium - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastrin</topic><topic>Gastrins - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Intestine. Mesentery</topic><topic>Isoquinolines - pharmacology</topic><topic>Kinetics</topic><topic>Male</topic><topic>Methionine - metabolism</topic><topic>Piperazines - pharmacology</topic><topic>PMA</topic><topic>Protein Biosynthesis</topic><topic>Protein kinase C</topic><topic>Protein Kinase C - antagonists & inhibitors</topic><topic>Protein Kinase C - metabolism</topic><topic>Protein synthesis</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yassin, Rihab R.</creatorcontrib><creatorcontrib>Murthy, S.N.S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Peptides (New York, N.Y. : 1980)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yassin, Rihab R.</au><au>Murthy, S.N.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Possible involvement of protein kinase C in mediating gastrin-induced response in rat colonic epithelium</atitle><jtitle>Peptides (New York, N.Y. : 1980)</jtitle><addtitle>Peptides</addtitle><date>1991-09-01</date><risdate>1991</risdate><volume>12</volume><issue>5</issue><spage>925</spage><epage>927</epage><pages>925-927</pages><issn>0196-9781</issn><eissn>1873-5169</eissn><coden>PPTDD5</coden><abstract>We examined the potential role of protein kinase C in signal transduction induced by gastrin's stimulation of rat colonic epithelium. Protein synthesis ([
35S]methionine incorporation into protein) and enzyme activity (decrease in the cytosolic activity) were measured following epithelial stimulation with gastrin. Gastrin (10 nM) increased [
35S]methionine incorporation into protein to 265% above maintenance level. The effect of gastrin was comparable to the stimulation induced by phorbol 12-myristate, 13-acetate (PMA), a strong activator of protein kinase C. The increase in protein synthesis induced by gastrin was totally abolished by 1-(5-isoquinolinyl)-2-methylpiperazine, an inhibitor of protein kinase C activity. Gastrin also decreased the cytosolic activity of the enzyme, an index of its activation and subsequent translocation to other cellular compartments. Therefore, we conclude that gastrin may be acting through a protein kinase C mechanism.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>1800956</pmid><doi>10.1016/0196-9781(91)90039-R</doi><tpages>3</tpages></addata></record> |
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| issn | 0196-9781 1873-5169 |
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| subjects | 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine Analysis of Variance Animals Biological and medical sciences colon Colon - drug effects Colon - enzymology Colon - metabolism Epithelium - drug effects Epithelium - enzymology Epithelium - metabolism Fundamental and applied biological sciences. Psychology Gastrin Gastrins - pharmacology In Vitro Techniques Intestine. Mesentery Isoquinolines - pharmacology Kinetics Male Methionine - metabolism Piperazines - pharmacology PMA Protein Biosynthesis Protein kinase C Protein Kinase C - antagonists & inhibitors Protein Kinase C - metabolism Protein synthesis Rats Rats, Inbred Strains Tetradecanoylphorbol Acetate - pharmacology Vertebrates: digestive system |
| title | Possible involvement of protein kinase C in mediating gastrin-induced response in rat colonic epithelium |
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