Non-inducible, mainly cell-associated β-lactamase from Nocardia asteroides strain 108
The beta-lactamase of the soil-borne strain 108 (parental strain) of Nocardia asteroides is a non-inducible enzyme mainly associated with the cells; it can be efficiently extracted by ultrasonication and SDS treatment. Crude enzyme preparations showed penicillinase and cephalosporinase activity. The...
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 1997-07, Vol.40 (1), p.5-11 |
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| container_title | Journal of antimicrobial chemotherapy |
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| creator | SCOPETTI, F FATTORINI, L FRANCESCHINI, N AMICOSANTE, G OREFICI, G |
| description | The beta-lactamase of the soil-borne strain 108 (parental strain) of Nocardia asteroides is a non-inducible enzyme mainly associated with the cells; it can be efficiently extracted by ultrasonication and SDS treatment. Crude enzyme preparations showed penicillinase and cephalosporinase activity. The kinetics of beta-lactamase production and in-vitro susceptibility to combinations of beta-lactam antibiotics plus beta-lactamase inhibitors have been studied in two stable overproducer mutants (A14 and B1) obtained by mutagenization of the parental strain with nitrosoguanidine. The cell-associated enzyme increased with bacterial growth in parental and mutant strains and was particularly abundant in stationary phase cells. The beta-lactamase inhibitors sulbactam and clavulanic acid decreased MIC values of penicillins more efficiently in the parental strain than in mutants, thus indicating some involvement of the enzyme in the resistance of N. asteroides strain 108 to beta-lactam antibiotics. |
| doi_str_mv | 10.1093/jac/40.1.5 |
| format | Article |
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Crude enzyme preparations showed penicillinase and cephalosporinase activity. The kinetics of beta-lactamase production and in-vitro susceptibility to combinations of beta-lactam antibiotics plus beta-lactamase inhibitors have been studied in two stable overproducer mutants (A14 and B1) obtained by mutagenization of the parental strain with nitrosoguanidine. The cell-associated enzyme increased with bacterial growth in parental and mutant strains and was particularly abundant in stationary phase cells. The beta-lactamase inhibitors sulbactam and clavulanic acid decreased MIC values of penicillins more efficiently in the parental strain than in mutants, thus indicating some involvement of the enzyme in the resistance of N. asteroides strain 108 to beta-lactam antibiotics.</description><identifier>ISSN: 0305-7453</identifier><identifier>ISSN: 1460-2091</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/40.1.5</identifier><identifier>PMID: 9249198</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Amoxicillin - pharmacology ; Bacteriology ; beta-Lactam Resistance - physiology ; beta-Lactamases - drug effects ; beta-Lactamases - genetics ; beta-Lactamases - metabolism ; beta-Lactams - metabolism ; beta-Lactams - pharmacology ; Biological and medical sciences ; Cefoxitin - pharmacology ; Detergents - pharmacology ; Fundamental and applied biological sciences. Psychology ; Hydrolysis ; Isoelectric Point ; Microbiology ; Mutagenesis ; Mutation ; Nocardia asteroides ; Nocardia asteroides - drug effects ; Nocardia asteroides - enzymology ; Nocardia asteroides - genetics ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Penicillin G - pharmacology</subject><ispartof>Journal of antimicrobial chemotherapy, 1997-07, Vol.40 (1), p.5-11</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-664360bd05dde86275c1c988899248053c13f661a13d0d80bb4d57a47a8aa76a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2750853$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9249198$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SCOPETTI, F</creatorcontrib><creatorcontrib>FATTORINI, L</creatorcontrib><creatorcontrib>FRANCESCHINI, N</creatorcontrib><creatorcontrib>AMICOSANTE, G</creatorcontrib><creatorcontrib>OREFICI, G</creatorcontrib><title>Non-inducible, mainly cell-associated β-lactamase from Nocardia asteroides strain 108</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>The beta-lactamase of the soil-borne strain 108 (parental strain) of Nocardia asteroides is a non-inducible enzyme mainly associated with the cells; it can be efficiently extracted by ultrasonication and SDS treatment. Crude enzyme preparations showed penicillinase and cephalosporinase activity. The kinetics of beta-lactamase production and in-vitro susceptibility to combinations of beta-lactam antibiotics plus beta-lactamase inhibitors have been studied in two stable overproducer mutants (A14 and B1) obtained by mutagenization of the parental strain with nitrosoguanidine. The cell-associated enzyme increased with bacterial growth in parental and mutant strains and was particularly abundant in stationary phase cells. The beta-lactamase inhibitors sulbactam and clavulanic acid decreased MIC values of penicillins more efficiently in the parental strain than in mutants, thus indicating some involvement of the enzyme in the resistance of N. asteroides strain 108 to beta-lactam antibiotics.</description><subject>Amoxicillin - pharmacology</subject><subject>Bacteriology</subject><subject>beta-Lactam Resistance - physiology</subject><subject>beta-Lactamases - drug effects</subject><subject>beta-Lactamases - genetics</subject><subject>beta-Lactamases - metabolism</subject><subject>beta-Lactams - metabolism</subject><subject>beta-Lactams - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cefoxitin - pharmacology</subject><subject>Detergents - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolysis</subject><subject>Isoelectric Point</subject><subject>Microbiology</subject><subject>Mutagenesis</subject><subject>Mutation</subject><subject>Nocardia asteroides</subject><subject>Nocardia asteroides - drug effects</subject><subject>Nocardia asteroides - enzymology</subject><subject>Nocardia asteroides - genetics</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Penicillin G - pharmacology</subject><issn>0305-7453</issn><issn>1460-2091</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOwzAQRS0EKqWwYY-UBWKBSDuOH3GWqOIlVWUDbKOJ7Uiu8ih2uuhv8SF8E66IuporzZmrO5eQawpzCgVbbFAveNRzcUKmlEtIMyjoKZkCA5HmXLBzchHCBgCkkGpCJkXGC1qoKfla913qOrPTrmrsQ9Ki65p9om3TpBhCrx0O1iS_P2mDesAWg01q37fJutfojcMEw2B974wNSRh8PE8oqEtyVmMT7NU4Z-Tz-elj-Zqu3l_elo-rVDOeDamUnEmoDAhjrJJZLjTVhVKqiAEVCKYpq6WkSJkBo6CquBE58hwVYi6Rzcjdv-_W9987G4aydeEQHjvb70JJJQVG8yyC9_-g9n0I3tbl1rsW_b6kUB5KLGOJJY-6FBG-GV13VWvNER1bi_vbcY9BY1N77LQLRyy-AUow9gdOBHk4</recordid><startdate>19970701</startdate><enddate>19970701</enddate><creator>SCOPETTI, F</creator><creator>FATTORINI, L</creator><creator>FRANCESCHINI, N</creator><creator>AMICOSANTE, G</creator><creator>OREFICI, G</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19970701</creationdate><title>Non-inducible, mainly cell-associated β-lactamase from Nocardia asteroides strain 108</title><author>SCOPETTI, F ; FATTORINI, L ; FRANCESCHINI, N ; AMICOSANTE, G ; OREFICI, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-664360bd05dde86275c1c988899248053c13f661a13d0d80bb4d57a47a8aa76a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amoxicillin - pharmacology</topic><topic>Bacteriology</topic><topic>beta-Lactam Resistance - physiology</topic><topic>beta-Lactamases - drug effects</topic><topic>beta-Lactamases - genetics</topic><topic>beta-Lactamases - metabolism</topic><topic>beta-Lactams - metabolism</topic><topic>beta-Lactams - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cefoxitin - pharmacology</topic><topic>Detergents - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolysis</topic><topic>Isoelectric Point</topic><topic>Microbiology</topic><topic>Mutagenesis</topic><topic>Mutation</topic><topic>Nocardia asteroides</topic><topic>Nocardia asteroides - drug effects</topic><topic>Nocardia asteroides - enzymology</topic><topic>Nocardia asteroides - genetics</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Penicillin G - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SCOPETTI, F</creatorcontrib><creatorcontrib>FATTORINI, L</creatorcontrib><creatorcontrib>FRANCESCHINI, N</creatorcontrib><creatorcontrib>AMICOSANTE, G</creatorcontrib><creatorcontrib>OREFICI, G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SCOPETTI, F</au><au>FATTORINI, L</au><au>FRANCESCHINI, N</au><au>AMICOSANTE, G</au><au>OREFICI, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Non-inducible, mainly cell-associated β-lactamase from Nocardia asteroides strain 108</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>1997-07-01</date><risdate>1997</risdate><volume>40</volume><issue>1</issue><spage>5</spage><epage>11</epage><pages>5-11</pages><issn>0305-7453</issn><issn>1460-2091</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>The beta-lactamase of the soil-borne strain 108 (parental strain) of Nocardia asteroides is a non-inducible enzyme mainly associated with the cells; it can be efficiently extracted by ultrasonication and SDS treatment. Crude enzyme preparations showed penicillinase and cephalosporinase activity. The kinetics of beta-lactamase production and in-vitro susceptibility to combinations of beta-lactam antibiotics plus beta-lactamase inhibitors have been studied in two stable overproducer mutants (A14 and B1) obtained by mutagenization of the parental strain with nitrosoguanidine. The cell-associated enzyme increased with bacterial growth in parental and mutant strains and was particularly abundant in stationary phase cells. The beta-lactamase inhibitors sulbactam and clavulanic acid decreased MIC values of penicillins more efficiently in the parental strain than in mutants, thus indicating some involvement of the enzyme in the resistance of N. asteroides strain 108 to beta-lactam antibiotics.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>9249198</pmid><doi>10.1093/jac/40.1.5</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of antimicrobial chemotherapy, 1997-07, Vol.40 (1), p.5-11 |
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| language | eng |
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| source | MEDLINE; Full-Text Journals in Chemistry (Open access); EZB Electronic Journals Library; Oxford Academic |
| subjects | Amoxicillin - pharmacology Bacteriology beta-Lactam Resistance - physiology beta-Lactamases - drug effects beta-Lactamases - genetics beta-Lactamases - metabolism beta-Lactams - metabolism beta-Lactams - pharmacology Biological and medical sciences Cefoxitin - pharmacology Detergents - pharmacology Fundamental and applied biological sciences. Psychology Hydrolysis Isoelectric Point Microbiology Mutagenesis Mutation Nocardia asteroides Nocardia asteroides - drug effects Nocardia asteroides - enzymology Nocardia asteroides - genetics Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Penicillin G - pharmacology |
| title | Non-inducible, mainly cell-associated β-lactamase from Nocardia asteroides strain 108 |
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