Mutational Analysis of Potential Zinc-Binding Residues in the Active Site of the Enterococcal d-Ala-d-Ala Dipeptidase VanX

VanX, one of the five proteins required for the vancomycin-resistant phenotype in clinically pathogenic Enterococci, is a zinc-containing d-Ala-d-Ala dipeptidase. To identify potential zinc ligands and begin defining the active site residues, we have mutated the 2 cysteine, 5 histidine, and 4 of the...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemistry (Easton) 1997-08, Vol.36 (34), p.10498-10505
Hauptverfasser:	McCafferty, Dewey G, Lessard, Ivan A. D, Walsh, Christopher T
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence ATP-Binding Cassette Transporters Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Binding Sites Carrier Proteins - genetics Circular Dichroism Conserved Sequence Dipeptidases - chemistry Dipeptidases - genetics Dipeptidases - metabolism DNA Mutational Analysis Enterococcus Enterococcus - enzymology Enterococcus - genetics Escherichia coli - genetics Escherichia coli Proteins Kinetics Maltose-Binding Proteins Models, Chemical Molecular Sequence Data Monosaccharide Transport Proteins Mutagenesis, Site-Directed Protein Conformation Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism Sequence Alignment Serine-Type D-Ala-D-Ala Carboxypeptidase Thermolysin - chemistry Thrombin - metabolism Zinc - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!