Detection of DNA lesions induced by chemical mutagens using the single-cell gel electrophoresis (Comet) assay.: 2. Relationship between DNA migration and alkaline condition
The alkaline condition is an important factor for the alkaline single-cell gel electrophoresis (SCG) assay to detect the genotoxic effects of chemicals. In order to understand the relationship between DNA migration and alkaline condition, the effect of 13 model chemical mutagens with different modes...
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| Veröffentlicht in: | Mutation research 1997-09, Vol.393 (1), p.107-113 |
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| creator | Miyamae, Youichi Iwasaki, Kouichi Kinae, Naohide Tsuda, Shuji Murakami, Michiko Tanaka, Makiko Sasaki, Yu F |
| description | The alkaline condition is an important factor for the alkaline single-cell gel electrophoresis (SCG) assay to detect the genotoxic effects of chemicals. In order to understand the relationship between DNA migration and alkaline condition, the effect of 13 model chemical mutagens with different modes of action was evaluated with the alkaline SCG assay under two different alkaline conditions (pH 12.1 and 12.6). CHO cells were sampled just after treatment for 1 h. The X-ray mimetic mutagen BLM increased DNA migration at pH 12.1 and 12.6 and the results were the same at both pH values. Six alkylating mutagens MNU, ENU, MNNG, ENNG, MMS, and EMS and one base adduct inducer 4-NQO induced a dose-dependent response only at pH 12.6. Two DNA crosslinking agents, MMC and DDP, and AMD had negative results. MMC and DDP, however, reduced the positive response of BLM, suggesting that DNA crosslinks could be detected. These results demonstrated that the alkaline condition was important factor for the alkaline SCG assay to detect the genotoxic effects of chemicals. |
| doi_str_mv | 10.1016/S1383-5718(97)00091-0 |
| format | Article |
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Relationship between DNA migration and alkaline condition</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Miyamae, Youichi ; Iwasaki, Kouichi ; Kinae, Naohide ; Tsuda, Shuji ; Murakami, Michiko ; Tanaka, Makiko ; Sasaki, Yu F</creator><creatorcontrib>Miyamae, Youichi ; Iwasaki, Kouichi ; Kinae, Naohide ; Tsuda, Shuji ; Murakami, Michiko ; Tanaka, Makiko ; Sasaki, Yu F</creatorcontrib><description>The alkaline condition is an important factor for the alkaline single-cell gel electrophoresis (SCG) assay to detect the genotoxic effects of chemicals. In order to understand the relationship between DNA migration and alkaline condition, the effect of 13 model chemical mutagens with different modes of action was evaluated with the alkaline SCG assay under two different alkaline conditions (pH 12.1 and 12.6). CHO cells were sampled just after treatment for 1 h. The X-ray mimetic mutagen BLM increased DNA migration at pH 12.1 and 12.6 and the results were the same at both pH values. Six alkylating mutagens MNU, ENU, MNNG, ENNG, MMS, and EMS and one base adduct inducer 4-NQO induced a dose-dependent response only at pH 12.6. Two DNA crosslinking agents, MMC and DDP, and AMD had negative results. MMC and DDP, however, reduced the positive response of BLM, suggesting that DNA crosslinks could be detected. These results demonstrated that the alkaline condition was important factor for the alkaline SCG assay to detect the genotoxic effects of chemicals.</description><identifier>ISSN: 1383-5718</identifier><identifier>ISSN: 0027-5107</identifier><identifier>EISSN: 1879-3592</identifier><identifier>DOI: 10.1016/S1383-5718(97)00091-0</identifier><identifier>PMID: 9357567</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Alkaline single cell gel electrophoresis (SCG) assay ; Alkylating Agents - toxicity ; Animals ; Chemical mutagen ; CHO Cells ; Comet assay ; Cricetinae ; Cross-Linking Reagents - toxicity ; DNA - drug effects ; DNA - genetics ; DNA - isolation & purification ; DNA Damage ; DNA lesion ; Electrophoresis - methods ; Evaluation Studies as Topic ; Hydrogen-Ion Concentration ; Mutagenicity Tests - methods ; Mutagens - toxicity</subject><ispartof>Mutation research, 1997-09, Vol.393 (1), p.107-113</ispartof><rights>1997 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1383571897000910$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9357567$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyamae, Youichi</creatorcontrib><creatorcontrib>Iwasaki, Kouichi</creatorcontrib><creatorcontrib>Kinae, Naohide</creatorcontrib><creatorcontrib>Tsuda, Shuji</creatorcontrib><creatorcontrib>Murakami, Michiko</creatorcontrib><creatorcontrib>Tanaka, Makiko</creatorcontrib><creatorcontrib>Sasaki, Yu F</creatorcontrib><title>Detection of DNA lesions induced by chemical mutagens using the single-cell gel electrophoresis (Comet) assay.: 2. Relationship between DNA migration and alkaline condition</title><title>Mutation research</title><addtitle>Mutat Res</addtitle><description>The alkaline condition is an important factor for the alkaline single-cell gel electrophoresis (SCG) assay to detect the genotoxic effects of chemicals. In order to understand the relationship between DNA migration and alkaline condition, the effect of 13 model chemical mutagens with different modes of action was evaluated with the alkaline SCG assay under two different alkaline conditions (pH 12.1 and 12.6). CHO cells were sampled just after treatment for 1 h. The X-ray mimetic mutagen BLM increased DNA migration at pH 12.1 and 12.6 and the results were the same at both pH values. Six alkylating mutagens MNU, ENU, MNNG, ENNG, MMS, and EMS and one base adduct inducer 4-NQO induced a dose-dependent response only at pH 12.6. Two DNA crosslinking agents, MMC and DDP, and AMD had negative results. MMC and DDP, however, reduced the positive response of BLM, suggesting that DNA crosslinks could be detected. These results demonstrated that the alkaline condition was important factor for the alkaline SCG assay to detect the genotoxic effects of chemicals.</description><subject>Alkaline single cell gel electrophoresis (SCG) assay</subject><subject>Alkylating Agents - toxicity</subject><subject>Animals</subject><subject>Chemical mutagen</subject><subject>CHO Cells</subject><subject>Comet assay</subject><subject>Cricetinae</subject><subject>Cross-Linking Reagents - toxicity</subject><subject>DNA - drug effects</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>DNA Damage</subject><subject>DNA lesion</subject><subject>Electrophoresis - methods</subject><subject>Evaluation Studies as Topic</subject><subject>Hydrogen-Ion Concentration</subject><subject>Mutagenicity Tests - methods</subject><subject>Mutagens - toxicity</subject><issn>1383-5718</issn><issn>0027-5107</issn><issn>1879-3592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1UV1v1DAQtBCotIWfUGmfUPuQY20nccwLqq4UKlWtxMez5cSbO4OTHHECuv_Ej8S5Xp921zOaXc8wdsFxxZGX779xWcmsULy61OoKETXP8AU75ZXSmSy0eJn6Z8prdhbjT0SBEqsTdqJloYpSnbJ_NzRRM_mhh6GFm4drCBTTFMH3bm7IQb2HZkudb2yAbp7shhI4R99vYNoSLE2grKEQYEMBKCS5cdhthzEJRbhcDx1NV2BjtPvVBxAr-ErBLhvj1u-gpukvUX9Y3fnNeEDA9g5s-GWD7wmaoXd-eX7DXrU2RHp7rOfsx-2n7-sv2f3j57v19X1GQsspE4KokHnthNSUt0qgs6rOudRVoxBb54oaC5HztkWbnKqksyXlktcKbV2RPGfvnnR34_B7pjiZzsflh7anYY6Gl1gWldSJeHEkznVHzuxG39lxb472JvzjE07p2j-eRhMbT32y1Y_JJuMGbziaJU9zyNMsYRmtzCFPg_I_EFGT2Q</recordid><startdate>19970918</startdate><enddate>19970918</enddate><creator>Miyamae, Youichi</creator><creator>Iwasaki, Kouichi</creator><creator>Kinae, Naohide</creator><creator>Tsuda, Shuji</creator><creator>Murakami, Michiko</creator><creator>Tanaka, Makiko</creator><creator>Sasaki, Yu F</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19970918</creationdate><title>Detection of DNA lesions induced by chemical mutagens using the single-cell gel electrophoresis (Comet) assay.: 2. Relationship between DNA migration and alkaline condition</title><author>Miyamae, Youichi ; Iwasaki, Kouichi ; Kinae, Naohide ; Tsuda, Shuji ; Murakami, Michiko ; Tanaka, Makiko ; Sasaki, Yu F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e293t-22ee534bd239e4f720da7b41398c700fdd5b05241ff0a35983da6e431b70ab8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Alkaline single cell gel electrophoresis (SCG) assay</topic><topic>Alkylating Agents - toxicity</topic><topic>Animals</topic><topic>Chemical mutagen</topic><topic>CHO Cells</topic><topic>Comet assay</topic><topic>Cricetinae</topic><topic>Cross-Linking Reagents - toxicity</topic><topic>DNA - drug effects</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>DNA Damage</topic><topic>DNA lesion</topic><topic>Electrophoresis - methods</topic><topic>Evaluation Studies as Topic</topic><topic>Hydrogen-Ion Concentration</topic><topic>Mutagenicity Tests - methods</topic><topic>Mutagens - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miyamae, Youichi</creatorcontrib><creatorcontrib>Iwasaki, Kouichi</creatorcontrib><creatorcontrib>Kinae, Naohide</creatorcontrib><creatorcontrib>Tsuda, Shuji</creatorcontrib><creatorcontrib>Murakami, Michiko</creatorcontrib><creatorcontrib>Tanaka, Makiko</creatorcontrib><creatorcontrib>Sasaki, Yu F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Mutation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyamae, Youichi</au><au>Iwasaki, Kouichi</au><au>Kinae, Naohide</au><au>Tsuda, Shuji</au><au>Murakami, Michiko</au><au>Tanaka, Makiko</au><au>Sasaki, Yu F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of DNA lesions induced by chemical mutagens using the single-cell gel electrophoresis (Comet) assay.: 2. Relationship between DNA migration and alkaline condition</atitle><jtitle>Mutation research</jtitle><addtitle>Mutat Res</addtitle><date>1997-09-18</date><risdate>1997</risdate><volume>393</volume><issue>1</issue><spage>107</spage><epage>113</epage><pages>107-113</pages><issn>1383-5718</issn><issn>0027-5107</issn><eissn>1879-3592</eissn><abstract>The alkaline condition is an important factor for the alkaline single-cell gel electrophoresis (SCG) assay to detect the genotoxic effects of chemicals. In order to understand the relationship between DNA migration and alkaline condition, the effect of 13 model chemical mutagens with different modes of action was evaluated with the alkaline SCG assay under two different alkaline conditions (pH 12.1 and 12.6). CHO cells were sampled just after treatment for 1 h. The X-ray mimetic mutagen BLM increased DNA migration at pH 12.1 and 12.6 and the results were the same at both pH values. Six alkylating mutagens MNU, ENU, MNNG, ENNG, MMS, and EMS and one base adduct inducer 4-NQO induced a dose-dependent response only at pH 12.6. Two DNA crosslinking agents, MMC and DDP, and AMD had negative results. MMC and DDP, however, reduced the positive response of BLM, suggesting that DNA crosslinks could be detected. These results demonstrated that the alkaline condition was important factor for the alkaline SCG assay to detect the genotoxic effects of chemicals.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9357567</pmid><doi>10.1016/S1383-5718(97)00091-0</doi><tpages>7</tpages></addata></record> |
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| subjects | Alkaline single cell gel electrophoresis (SCG) assay Alkylating Agents - toxicity Animals Chemical mutagen CHO Cells Comet assay Cricetinae Cross-Linking Reagents - toxicity DNA - drug effects DNA - genetics DNA - isolation & purification DNA Damage DNA lesion Electrophoresis - methods Evaluation Studies as Topic Hydrogen-Ion Concentration Mutagenicity Tests - methods Mutagens - toxicity |
| title | Detection of DNA lesions induced by chemical mutagens using the single-cell gel electrophoresis (Comet) assay.: 2. Relationship between DNA migration and alkaline condition |
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