The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes
Penicillium chrysogenum DNA fragments cloned in EMBL3 or cosmid vectors from the upstream region of the pcbC-penDE cluster carry a gene (pcbAB) that complemented the deficiency of alpha-aminoadipyl-cysteinyl-valine synthetase of mutants npe5 and npe10, and restored penicillin production to mutant np...
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| Veröffentlicht in: | The Journal of biological chemistry 1990-09, Vol.265 (27), p.16358-16365 |
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| creator | Díez, B Gutiérrez, S Barredo, J L van Solingen, P van der Voort, L H Martín, J F |
| description | Penicillium chrysogenum DNA fragments cloned in EMBL3 or cosmid vectors from the upstream region of the pcbC-penDE cluster carry a gene (pcbAB) that complemented the deficiency of alpha-aminoadipyl-cysteinyl-valine synthetase of mutants npe5 and npe10, and restored penicillin production to mutant npe5. A protein of about 250 kDa was observed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels of cell-free extracts of complemented strains that was absent in the npe5 and npe10 mutants but exists in the parental strain from which the mutants were obtained. Transcriptional mapping studies showed the presence of one long transcript of about 11.5 kilobases that hybridized with several probes internal to the pcbAB gene, and two small transcripts of 1.15 kilobases that hybridized with the pcbC or the penDE gene, respectively. The transcription initiation and termination regions of the pcbAB gene were mapped by hybridization with several small probes. The region has been completely sequenced. It includes an open reading frame of 11,376 nucleotides that encodes a protein with a deduced Mr of 425,971. Three repeated dominia were found in the alpha-aminoadipyl-cysteinyl-valine synthetase which have high homology with the gramicidin synthetase I and tyrocidine synthetase I. The pcbAB is linked to the pcbC and penDE genes and is transcribed in the opposite orientation to them. |
| doi_str_mv | 10.1016/S0021-9258(17)46231-4 |
| format | Article |
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Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Díez, B ; Gutiérrez, S ; Barredo, J L ; van Solingen, P ; van der Voort, L H ; Martín, J F</creator><creatorcontrib>Díez, B ; Gutiérrez, S ; Barredo, J L ; van Solingen, P ; van der Voort, L H ; Martín, J F</creatorcontrib><description>Penicillium chrysogenum DNA fragments cloned in EMBL3 or cosmid vectors from the upstream region of the pcbC-penDE cluster carry a gene (pcbAB) that complemented the deficiency of alpha-aminoadipyl-cysteinyl-valine synthetase of mutants npe5 and npe10, and restored penicillin production to mutant npe5. A protein of about 250 kDa was observed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels of cell-free extracts of complemented strains that was absent in the npe5 and npe10 mutants but exists in the parental strain from which the mutants were obtained. Transcriptional mapping studies showed the presence of one long transcript of about 11.5 kilobases that hybridized with several probes internal to the pcbAB gene, and two small transcripts of 1.15 kilobases that hybridized with the pcbC or the penDE gene, respectively. The transcription initiation and termination regions of the pcbAB gene were mapped by hybridization with several small probes. The region has been completely sequenced. It includes an open reading frame of 11,376 nucleotides that encodes a protein with a deduced Mr of 425,971. Three repeated dominia were found in the alpha-aminoadipyl-cysteinyl-valine synthetase which have high homology with the gramicidin synthetase I and tyrocidine synthetase I. The pcbAB is linked to the pcbC and penDE genes and is transcribed in the opposite orientation to them.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(17)46231-4</identifier><identifier>PMID: 2129535</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>ACIDE ORGANIQUE ; ACIDOS ORGANICOS ; ADIPIC ACID ; alpha -aminoadipyl-cysteinyl-valine synthase ; Amino Acid Sequence ; AMINO ACID SEQUENCES ; Base Sequence ; Biological and medical sciences ; BIOSINTESIS ; BIOSYNTHESE ; BIOSYNTHESIS ; Blotting, Southern ; CISTEINA ; CLONACION ; CLONAGE ; CLONING ; Cloning, Molecular ; Cosmids ; CYSTEINE ; DNA, Fungal - genetics ; DNA, Fungal - isolation & purification ; embl/jo5604 ; Escherichia coli - genetics ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; genbank/jo5604 ; GENE EXPRESSION ; genes ; Genes, Fungal ; Genes. Genome ; Genetic Complementation Test ; Genetic Linkage ; Genetic Vectors ; LIGASAS ; LIGASE ; LIGASES ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Multigene Family ; Mutation ; NUCLEOTIDE ; NUCLEOTIDES ; NUCLEOTIDOS ; ORGANIC ACIDS ; PENICILINA ; penicillin ; PENICILLINE ; PENICILLINS ; Penicillins - biosynthesis ; PENICILLIUM ; Penicillium - genetics ; PENICILLIUM CHRYSOGENUM ; Penicillium chrysogenum - enzymology ; Penicillium chrysogenum - genetics ; PEPTIDE ; Peptide Synthases - genetics ; PEPTIDES ; PEPTIDOS ; Restriction Mapping ; Sequence Homology, Nucleic Acid ; Transcription, Genetic ; VALINA ; VALINE</subject><ispartof>The Journal of biological chemistry, 1990-09, Vol.265 (27), p.16358-16365</ispartof><rights>1990 © 1990 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-80d4eb38870ebd6a90c68f1f1dc379243c75363ace3cd78bf51a1887cd8eda753</citedby><cites>FETCH-LOGICAL-c485t-80d4eb38870ebd6a90c68f1f1dc379243c75363ace3cd78bf51a1887cd8eda753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19383913$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2129535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Díez, B</creatorcontrib><creatorcontrib>Gutiérrez, S</creatorcontrib><creatorcontrib>Barredo, J L</creatorcontrib><creatorcontrib>van Solingen, P</creatorcontrib><creatorcontrib>van der Voort, L H</creatorcontrib><creatorcontrib>Martín, J F</creatorcontrib><title>The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Penicillium chrysogenum DNA fragments cloned in EMBL3 or cosmid vectors from the upstream region of the pcbC-penDE cluster carry a gene (pcbAB) that complemented the deficiency of alpha-aminoadipyl-cysteinyl-valine synthetase of mutants npe5 and npe10, and restored penicillin production to mutant npe5. A protein of about 250 kDa was observed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels of cell-free extracts of complemented strains that was absent in the npe5 and npe10 mutants but exists in the parental strain from which the mutants were obtained. Transcriptional mapping studies showed the presence of one long transcript of about 11.5 kilobases that hybridized with several probes internal to the pcbAB gene, and two small transcripts of 1.15 kilobases that hybridized with the pcbC or the penDE gene, respectively. The transcription initiation and termination regions of the pcbAB gene were mapped by hybridization with several small probes. The region has been completely sequenced. It includes an open reading frame of 11,376 nucleotides that encodes a protein with a deduced Mr of 425,971. Three repeated dominia were found in the alpha-aminoadipyl-cysteinyl-valine synthetase which have high homology with the gramicidin synthetase I and tyrocidine synthetase I. The pcbAB is linked to the pcbC and penDE genes and is transcribed in the opposite orientation to them.</description><subject>ACIDE ORGANIQUE</subject><subject>ACIDOS ORGANICOS</subject><subject>ADIPIC ACID</subject><subject>alpha -aminoadipyl-cysteinyl-valine synthase</subject><subject>Amino Acid Sequence</subject><subject>AMINO ACID SEQUENCES</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>BIOSYNTHESIS</subject><subject>Blotting, Southern</subject><subject>CISTEINA</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>CLONING</subject><subject>Cloning, Molecular</subject><subject>Cosmids</subject><subject>CYSTEINE</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Fungal - isolation & purification</subject><subject>embl/jo5604</subject><subject>Escherichia coli - genetics</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genbank/jo5604</subject><subject>GENE EXPRESSION</subject><subject>genes</subject><subject>Genes, Fungal</subject><subject>Genes. Genome</subject><subject>Genetic Complementation Test</subject><subject>Genetic Linkage</subject><subject>Genetic Vectors</subject><subject>LIGASAS</subject><subject>LIGASE</subject><subject>LIGASES</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Mutation</subject><subject>NUCLEOTIDE</subject><subject>NUCLEOTIDES</subject><subject>NUCLEOTIDOS</subject><subject>ORGANIC ACIDS</subject><subject>PENICILINA</subject><subject>penicillin</subject><subject>PENICILLINE</subject><subject>PENICILLINS</subject><subject>Penicillins - biosynthesis</subject><subject>PENICILLIUM</subject><subject>Penicillium - genetics</subject><subject>PENICILLIUM CHRYSOGENUM</subject><subject>Penicillium chrysogenum - enzymology</subject><subject>Penicillium chrysogenum - genetics</subject><subject>PEPTIDE</subject><subject>Peptide Synthases - genetics</subject><subject>PEPTIDES</subject><subject>PEPTIDOS</subject><subject>Restriction Mapping</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Transcription, Genetic</subject><subject>VALINA</subject><subject>VALINE</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUctu1DAUjRColMIPIFXyAhAsUuw4zxUqQ4FKlVi0ldhZN9c3E0PGDnamaPhAvgtPMpQl2Tg69zyufZLkVPAzwUX59przTKRNVtSvRfUmLzMp0vxBcix4LVNZiK8Pk-N7yuPkSQjfePzyRhwlR5nImkIWx8nvm54YDtswkWeuYyNZg2YYjGWtcWFnp54mg2xNlsIZu9RkJ9MZhMk4y8Bqhj14wCg3vxYwukQRG7E9fz_rGFl02tj1jMMw9pDCxlgH2oy7IcVdTDc2_t1BDCZ2iIVAc0LEvsOa2OT-Gq9mPO764WLZ7GnyqIMh0LPDeZLcfry4WX1Or758ulydX6WY18WU1lzn1Mq6rji1uoSGY1l3ohMaZdVkucSqkKUEJIm6qtuuECAiG3VNGuLsJHm1-I7e_dhSmNTGBKRhAEtuG5QoeZnnvIzEYiGidyF46tTozQb8Tgmu9v2puT-1L0eJSs39qTzqTg8B23ZD-l51KCzOXx7mEBCGzoNFE_6ZN7KWjZCR92Lh9Wbd_zSeVKwTe9qorCxUVsVNZVFH2vOF1oFTsPbR6va6EVzKau_xbhlSfNE7Q14FNLFL0tEPJ6Wd-c9t_gCKgM_Y</recordid><startdate>19900925</startdate><enddate>19900925</enddate><creator>Díez, B</creator><creator>Gutiérrez, S</creator><creator>Barredo, J L</creator><creator>van Solingen, P</creator><creator>van der Voort, L H</creator><creator>Martín, J F</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19900925</creationdate><title>The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes</title><author>Díez, B ; Gutiérrez, S ; Barredo, J L ; van Solingen, P ; van der Voort, L H ; Martín, J F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-80d4eb38870ebd6a90c68f1f1dc379243c75363ace3cd78bf51a1887cd8eda753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>ACIDE ORGANIQUE</topic><topic>ACIDOS ORGANICOS</topic><topic>ADIPIC ACID</topic><topic>alpha -aminoadipyl-cysteinyl-valine synthase</topic><topic>Amino Acid Sequence</topic><topic>AMINO ACID SEQUENCES</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>BIOSYNTHESIS</topic><topic>Blotting, Southern</topic><topic>CISTEINA</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>CLONING</topic><topic>Cloning, Molecular</topic><topic>Cosmids</topic><topic>CYSTEINE</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Fungal - isolation & purification</topic><topic>embl/jo5604</topic><topic>Escherichia coli - genetics</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genbank/jo5604</topic><topic>GENE EXPRESSION</topic><topic>genes</topic><topic>Genes, Fungal</topic><topic>Genes. Genome</topic><topic>Genetic Complementation Test</topic><topic>Genetic Linkage</topic><topic>Genetic Vectors</topic><topic>LIGASAS</topic><topic>LIGASE</topic><topic>LIGASES</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Mutation</topic><topic>NUCLEOTIDE</topic><topic>NUCLEOTIDES</topic><topic>NUCLEOTIDOS</topic><topic>ORGANIC ACIDS</topic><topic>PENICILINA</topic><topic>penicillin</topic><topic>PENICILLINE</topic><topic>PENICILLINS</topic><topic>Penicillins - biosynthesis</topic><topic>PENICILLIUM</topic><topic>Penicillium - genetics</topic><topic>PENICILLIUM CHRYSOGENUM</topic><topic>Penicillium chrysogenum - enzymology</topic><topic>Penicillium chrysogenum - genetics</topic><topic>PEPTIDE</topic><topic>Peptide Synthases - genetics</topic><topic>PEPTIDES</topic><topic>PEPTIDOS</topic><topic>Restriction Mapping</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Transcription, Genetic</topic><topic>VALINA</topic><topic>VALINE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Díez, B</creatorcontrib><creatorcontrib>Gutiérrez, S</creatorcontrib><creatorcontrib>Barredo, J L</creatorcontrib><creatorcontrib>van Solingen, P</creatorcontrib><creatorcontrib>van der Voort, L H</creatorcontrib><creatorcontrib>Martín, J F</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Díez, B</au><au>Gutiérrez, S</au><au>Barredo, J L</au><au>van Solingen, P</au><au>van der Voort, L H</au><au>Martín, J F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1990-09-25</date><risdate>1990</risdate><volume>265</volume><issue>27</issue><spage>16358</spage><epage>16365</epage><pages>16358-16365</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Penicillium chrysogenum DNA fragments cloned in EMBL3 or cosmid vectors from the upstream region of the pcbC-penDE cluster carry a gene (pcbAB) that complemented the deficiency of alpha-aminoadipyl-cysteinyl-valine synthetase of mutants npe5 and npe10, and restored penicillin production to mutant npe5. A protein of about 250 kDa was observed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels of cell-free extracts of complemented strains that was absent in the npe5 and npe10 mutants but exists in the parental strain from which the mutants were obtained. Transcriptional mapping studies showed the presence of one long transcript of about 11.5 kilobases that hybridized with several probes internal to the pcbAB gene, and two small transcripts of 1.15 kilobases that hybridized with the pcbC or the penDE gene, respectively. The transcription initiation and termination regions of the pcbAB gene were mapped by hybridization with several small probes. The region has been completely sequenced. It includes an open reading frame of 11,376 nucleotides that encodes a protein with a deduced Mr of 425,971. Three repeated dominia were found in the alpha-aminoadipyl-cysteinyl-valine synthetase which have high homology with the gramicidin synthetase I and tyrocidine synthetase I. The pcbAB is linked to the pcbC and penDE genes and is transcribed in the opposite orientation to them.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>2129535</pmid><doi>10.1016/S0021-9258(17)46231-4</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1990-09, Vol.265 (27), p.16358-16365 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16064406 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | ACIDE ORGANIQUE ACIDOS ORGANICOS ADIPIC ACID alpha -aminoadipyl-cysteinyl-valine synthase Amino Acid Sequence AMINO ACID SEQUENCES Base Sequence Biological and medical sciences BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS Blotting, Southern CISTEINA CLONACION CLONAGE CLONING Cloning, Molecular Cosmids CYSTEINE DNA, Fungal - genetics DNA, Fungal - isolation & purification embl/jo5604 Escherichia coli - genetics EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology genbank/jo5604 GENE EXPRESSION genes Genes, Fungal Genes. Genome Genetic Complementation Test Genetic Linkage Genetic Vectors LIGASAS LIGASE LIGASES Molecular and cellular biology Molecular genetics Molecular Sequence Data Multigene Family Mutation NUCLEOTIDE NUCLEOTIDES NUCLEOTIDOS ORGANIC ACIDS PENICILINA penicillin PENICILLINE PENICILLINS Penicillins - biosynthesis PENICILLIUM Penicillium - genetics PENICILLIUM CHRYSOGENUM Penicillium chrysogenum - enzymology Penicillium chrysogenum - genetics PEPTIDE Peptide Synthases - genetics PEPTIDES PEPTIDOS Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic VALINA VALINE |
| title | The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes |
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