Interferon-gamma transcriptionally modulates the expression of the genes for the high affinity IgG-Fc receptor and the 47-kDa cytosolic component of NADPH oxidase in human polymorphonuclear leukocytes
We examined the mechanisms responsible for the regulation by interferon-gamma (IFN-gamma) of the expression of the genes encoding the high affinity IgG-Fc receptor (Fc gamma R-I, CD64) and the NADPH oxidase 47-kDa cytosolic factor (p47-phox) in human polymorphonuclear leukocytes (PMN). Nuclear run-o...
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| Veröffentlicht in: | The Journal of biological chemistry 1991-11, Vol.266 (33), p.22079-22082 |
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| container_title | The Journal of biological chemistry |
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| creator | CASSATELLA, M. A BAZZONI, F CALZETTI, F GUASPARRI, I ROSSI, F TRINCHIERI, G |
| description | We examined the mechanisms responsible for the regulation by interferon-gamma (IFN-gamma) of the expression of the genes encoding
the high affinity IgG-Fc receptor (Fc gamma R-I, CD64) and the NADPH oxidase 47-kDa cytosolic factor (p47-phox) in human polymorphonuclear
leukocytes (PMN). Nuclear run-on transcriptional assays demonstrated that the Fc gamma R-I gene transcription is undetectable
in untreated PMN but is significantly induced by IFN-gamma. Unlike Fc gamma R-I, p47-phox gene transcription is constitutively
active in resting PMN and is down-regulated by a 2-h treatment of these cells with IFN-gamma. The transcriptional modulation
by IFN-gamma of Fc gamma R-I and p47-phox genes is not influenced by the protein synthesis inhibitor cycloheximide. Moreover,
Northern blot analysis revealed that cycloheximide superinduces p47-phox mRNA expression by increasing its half-life and without
affecting p47-phox gene transcription. These findings indicate that human PMN can regulate gene expression by transcriptional
and posttranscriptional events. |
| doi_str_mv | 10.1016/s0021-9258(18)54534-8 |
| format | Article |
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the high affinity IgG-Fc receptor (Fc gamma R-I, CD64) and the NADPH oxidase 47-kDa cytosolic factor (p47-phox) in human polymorphonuclear
leukocytes (PMN). Nuclear run-on transcriptional assays demonstrated that the Fc gamma R-I gene transcription is undetectable
in untreated PMN but is significantly induced by IFN-gamma. Unlike Fc gamma R-I, p47-phox gene transcription is constitutively
active in resting PMN and is down-regulated by a 2-h treatment of these cells with IFN-gamma. The transcriptional modulation
by IFN-gamma of Fc gamma R-I and p47-phox genes is not influenced by the protein synthesis inhibitor cycloheximide. Moreover,
Northern blot analysis revealed that cycloheximide superinduces p47-phox mRNA expression by increasing its half-life and without
affecting p47-phox gene transcription. These findings indicate that human PMN can regulate gene expression by transcriptional
and posttranscriptional events.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/s0021-9258(18)54534-8</identifier><identifier>PMID: 1834666</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Antigens, Differentiation - genetics ; Biological and medical sciences ; Cell Nucleus - drug effects ; Cell Nucleus - physiology ; Cells, Cultured ; Cycloheximide - pharmacology ; Cytosol - enzymology ; Dactinomycin - pharmacology ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression - drug effects ; Humans ; Immunoglobulin G - metabolism ; Interferon-gamma - pharmacology ; Kinetics ; Molecular and cellular biology ; Molecular genetics ; NADH, NADPH Oxidoreductases - blood ; NADH, NADPH Oxidoreductases - genetics ; NADPH Oxidases ; Neutrophils - drug effects ; Neutrophils - immunology ; Neutrophils - physiology ; Receptors, Fc - genetics ; Receptors, IgG ; Recombinant Proteins ; RNA, Messenger - blood ; RNA, Messenger - drug effects ; RNA, Messenger - genetics ; Transcription, Genetic - drug effects</subject><ispartof>The Journal of biological chemistry, 1991-11, Vol.266 (33), p.22079-22082</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-12af8dd9ff4d74c19674060550ae84fa427f867fab8de617b0929b694ff2ebd33</citedby><cites>FETCH-LOGICAL-c506t-12af8dd9ff4d74c19674060550ae84fa427f867fab8de617b0929b694ff2ebd33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5094861$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1834666$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CASSATELLA, M. A</creatorcontrib><creatorcontrib>BAZZONI, F</creatorcontrib><creatorcontrib>CALZETTI, F</creatorcontrib><creatorcontrib>GUASPARRI, I</creatorcontrib><creatorcontrib>ROSSI, F</creatorcontrib><creatorcontrib>TRINCHIERI, G</creatorcontrib><title>Interferon-gamma transcriptionally modulates the expression of the genes for the high affinity IgG-Fc receptor and the 47-kDa cytosolic component of NADPH oxidase in human polymorphonuclear leukocytes</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>We examined the mechanisms responsible for the regulation by interferon-gamma (IFN-gamma) of the expression of the genes encoding
the high affinity IgG-Fc receptor (Fc gamma R-I, CD64) and the NADPH oxidase 47-kDa cytosolic factor (p47-phox) in human polymorphonuclear
leukocytes (PMN). Nuclear run-on transcriptional assays demonstrated that the Fc gamma R-I gene transcription is undetectable
in untreated PMN but is significantly induced by IFN-gamma. Unlike Fc gamma R-I, p47-phox gene transcription is constitutively
active in resting PMN and is down-regulated by a 2-h treatment of these cells with IFN-gamma. The transcriptional modulation
by IFN-gamma of Fc gamma R-I and p47-phox genes is not influenced by the protein synthesis inhibitor cycloheximide. Moreover,
Northern blot analysis revealed that cycloheximide superinduces p47-phox mRNA expression by increasing its half-life and without
affecting p47-phox gene transcription. These findings indicate that human PMN can regulate gene expression by transcriptional
and posttranscriptional events.</description><subject>Antigens, Differentiation - genetics</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - physiology</subject><subject>Cells, Cultured</subject><subject>Cycloheximide - pharmacology</subject><subject>Cytosol - enzymology</subject><subject>Dactinomycin - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression - drug effects</subject><subject>Humans</subject><subject>Immunoglobulin G - metabolism</subject><subject>Interferon-gamma - pharmacology</subject><subject>Kinetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>NADH, NADPH Oxidoreductases - blood</subject><subject>NADH, NADPH Oxidoreductases - genetics</subject><subject>NADPH Oxidases</subject><subject>Neutrophils - drug effects</subject><subject>Neutrophils - immunology</subject><subject>Neutrophils - physiology</subject><subject>Receptors, Fc - genetics</subject><subject>Receptors, IgG</subject><subject>Recombinant Proteins</subject><subject>RNA, Messenger - blood</subject><subject>RNA, Messenger - drug effects</subject><subject>RNA, Messenger - genetics</subject><subject>Transcription, Genetic - drug effects</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkcFu1DAURSMEKtPCJ1TyAiG6CNiJ4zjLqqXtSBUgARI7y3GeJ6aOHWxHNH_IZ5HMjFpvLPued9_iZNk5wR8JJuxTxLggeVNU_APhFxWtSprzF9mGYF7mZUV-vcw2T8jr7DTG33g5tCEn2QnhJWWMbbJ_W5cgaAje5Ts5DBKlIF1UwYzJeCetndHgu8nKBBGlHhA8jgFiXELk9f5nB27JtA_7V292PZJaG2fSjLa72_xGoQAKxrQQ0nV7itb5w7VEak4-emsUUn4YvQOX1tYvl9ff7pB_NJ2MgIxD_TRIh0Zv58GHsfduUhZkQBamB7-UQHyTvdLSRnh7vM-ynzeff1zd5fdfb7dXl_e5qjBLOSmk5l3XaE27mirSsJpihqsKS-BUS1rUmrNay5Z3wEjd4qZoWtZQrQtou7I8y94fesfg_0wQkxhMVGCtdOCnKMhSxklJFrA6gCr4GANoMQYzyDALgsVqUHxf9YhVjyBc7A0KvsydHxdM7QDd89RB2ZK_O-YyKmn1okuZ-IRVuKGckWds9fHXBBCt8aqHQRSMibIURYHrpvwPxAS0dw</recordid><startdate>19911125</startdate><enddate>19911125</enddate><creator>CASSATELLA, M. A</creator><creator>BAZZONI, F</creator><creator>CALZETTI, F</creator><creator>GUASPARRI, I</creator><creator>ROSSI, F</creator><creator>TRINCHIERI, G</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>19911125</creationdate><title>Interferon-gamma transcriptionally modulates the expression of the genes for the high affinity IgG-Fc receptor and the 47-kDa cytosolic component of NADPH oxidase in human polymorphonuclear leukocytes</title><author>CASSATELLA, M. A ; BAZZONI, F ; CALZETTI, F ; GUASPARRI, I ; ROSSI, F ; TRINCHIERI, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-12af8dd9ff4d74c19674060550ae84fa427f867fab8de617b0929b694ff2ebd33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Antigens, Differentiation - genetics</topic><topic>Biological and medical sciences</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - physiology</topic><topic>Cells, Cultured</topic><topic>Cycloheximide - pharmacology</topic><topic>Cytosol - enzymology</topic><topic>Dactinomycin - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression - drug effects</topic><topic>Humans</topic><topic>Immunoglobulin G - metabolism</topic><topic>Interferon-gamma - pharmacology</topic><topic>Kinetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>NADH, NADPH Oxidoreductases - blood</topic><topic>NADH, NADPH Oxidoreductases - genetics</topic><topic>NADPH Oxidases</topic><topic>Neutrophils - drug effects</topic><topic>Neutrophils - immunology</topic><topic>Neutrophils - physiology</topic><topic>Receptors, Fc - genetics</topic><topic>Receptors, IgG</topic><topic>Recombinant Proteins</topic><topic>RNA, Messenger - blood</topic><topic>RNA, Messenger - drug effects</topic><topic>RNA, Messenger - genetics</topic><topic>Transcription, Genetic - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CASSATELLA, M. A</creatorcontrib><creatorcontrib>BAZZONI, F</creatorcontrib><creatorcontrib>CALZETTI, F</creatorcontrib><creatorcontrib>GUASPARRI, I</creatorcontrib><creatorcontrib>ROSSI, F</creatorcontrib><creatorcontrib>TRINCHIERI, G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CASSATELLA, M. A</au><au>BAZZONI, F</au><au>CALZETTI, F</au><au>GUASPARRI, I</au><au>ROSSI, F</au><au>TRINCHIERI, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interferon-gamma transcriptionally modulates the expression of the genes for the high affinity IgG-Fc receptor and the 47-kDa cytosolic component of NADPH oxidase in human polymorphonuclear leukocytes</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1991-11-25</date><risdate>1991</risdate><volume>266</volume><issue>33</issue><spage>22079</spage><epage>22082</epage><pages>22079-22082</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>We examined the mechanisms responsible for the regulation by interferon-gamma (IFN-gamma) of the expression of the genes encoding
the high affinity IgG-Fc receptor (Fc gamma R-I, CD64) and the NADPH oxidase 47-kDa cytosolic factor (p47-phox) in human polymorphonuclear
leukocytes (PMN). Nuclear run-on transcriptional assays demonstrated that the Fc gamma R-I gene transcription is undetectable
in untreated PMN but is significantly induced by IFN-gamma. Unlike Fc gamma R-I, p47-phox gene transcription is constitutively
active in resting PMN and is down-regulated by a 2-h treatment of these cells with IFN-gamma. The transcriptional modulation
by IFN-gamma of Fc gamma R-I and p47-phox genes is not influenced by the protein synthesis inhibitor cycloheximide. Moreover,
Northern blot analysis revealed that cycloheximide superinduces p47-phox mRNA expression by increasing its half-life and without
affecting p47-phox gene transcription. These findings indicate that human PMN can regulate gene expression by transcriptional
and posttranscriptional events.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>1834666</pmid><doi>10.1016/s0021-9258(18)54534-8</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1991-11, Vol.266 (33), p.22079-22082 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16058131 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Antigens, Differentiation - genetics Biological and medical sciences Cell Nucleus - drug effects Cell Nucleus - physiology Cells, Cultured Cycloheximide - pharmacology Cytosol - enzymology Dactinomycin - pharmacology Fundamental and applied biological sciences. Psychology Gene expression Gene Expression - drug effects Humans Immunoglobulin G - metabolism Interferon-gamma - pharmacology Kinetics Molecular and cellular biology Molecular genetics NADH, NADPH Oxidoreductases - blood NADH, NADPH Oxidoreductases - genetics NADPH Oxidases Neutrophils - drug effects Neutrophils - immunology Neutrophils - physiology Receptors, Fc - genetics Receptors, IgG Recombinant Proteins RNA, Messenger - blood RNA, Messenger - drug effects RNA, Messenger - genetics Transcription, Genetic - drug effects |
| title | Interferon-gamma transcriptionally modulates the expression of the genes for the high affinity IgG-Fc receptor and the 47-kDa cytosolic component of NADPH oxidase in human polymorphonuclear leukocytes |
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