The Production of Biologically Active 3-Hydroxy-5,8,11,14-Eicosa Tetraenoic Acid (3-HETE) and Linoleic Acid Metabolites by Dipodascopsis
3-Hydroxy-5,8,11,14-eicosatetraenoic acid (3-HETE) is a biologically active compound which has a signal transducing role in human neutrophils. When 12.5 mg/l arachidonic acid (AA) was fed to sexual reproductive cells of Dipodascopsis uninucleata and D. tóthii the former transformed approximately 4%...
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| Veröffentlicht in: | Systematic and applied microbiology 1997, Vol.20 (1), p.39-49 |
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| creator | Kock, J.L.F. van Vuuren, D. Jansen Botha, A. van Dyk, M.S. Coetzee, D.J. Botes, P.J. Shaw, N. Friend, J. Ratledge, C. Roberts, A.D. Nigam, S. |
| description | 3-Hydroxy-5,8,11,14-eicosatetraenoic acid (3-HETE) is a biologically active compound which has a signal transducing role in human neutrophils. When 12.5 mg/l arachidonic acid (AA) was fed to sexual reproductive cells of
Dipodascopsis uninucleata and
D. tóthii the former transformed approximately 4% of AA to 3-HETE, while the latter produced only trace amounts of 3-HETE. The 3-HETE was only associated with cellular ethanol extracts of both species.
D. uninucleata utilized 69% of the AA after 6h while
D. tóthii utilized 33%. The residual or unused AA was mainly present in the cells of
D. tóthii (96%) and
D. uninucleata (98%) and only small amounts (4% and 2%) in their respective supernatants. Extraction with 80% aqueous ethanol succeeded in removing respectively 92% and 45% of AA present in the cells of
D. tóthii and
D. uninucleata. The poor AA extraction from cells of
D. uninucleata contributed to the presence of AA in the neutral lipid fraction which is insoluble in ethanol. If efficient AA extraction can be implemented, approximately 67% and 31% unused AA can be recovered from
D. tóthii and
D. uninucleata respectively which can be recycled for biotransformation purposes. Although no traces of other 3-hydroxy fatty acids were detected, enzyme extracts from
D. uninucleata metabolised exogenous 18:2 in a manner consistent with the presence of a prostaglandin endoperoxide synthase similar to that in fetal calf blood vessels. |
| doi_str_mv | 10.1016/S0723-2020(97)80046-X |
| format | Article |
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Dipodascopsis uninucleata and
D. tóthii the former transformed approximately 4% of AA to 3-HETE, while the latter produced only trace amounts of 3-HETE. The 3-HETE was only associated with cellular ethanol extracts of both species.
D. uninucleata utilized 69% of the AA after 6h while
D. tóthii utilized 33%. The residual or unused AA was mainly present in the cells of
D. tóthii (96%) and
D. uninucleata (98%) and only small amounts (4% and 2%) in their respective supernatants. Extraction with 80% aqueous ethanol succeeded in removing respectively 92% and 45% of AA present in the cells of
D. tóthii and
D. uninucleata. The poor AA extraction from cells of
D. uninucleata contributed to the presence of AA in the neutral lipid fraction which is insoluble in ethanol. If efficient AA extraction can be implemented, approximately 67% and 31% unused AA can be recovered from
D. tóthii and
D. uninucleata respectively which can be recycled for biotransformation purposes. Although no traces of other 3-hydroxy fatty acids were detected, enzyme extracts from
D. uninucleata metabolised exogenous 18:2 in a manner consistent with the presence of a prostaglandin endoperoxide synthase similar to that in fetal calf blood vessels.</description><identifier>ISSN: 0723-2020</identifier><identifier>EISSN: 1618-0984</identifier><identifier>DOI: 10.1016/S0723-2020(97)80046-X</identifier><identifier>CODEN: SAMIDF</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>18:2-metabolites ; 3-HETE ; Arachidonic acid ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; D. tóthii ; D. uninucleata ; Dipodascopsis ; Fundamental and applied biological sciences. Psychology ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Microbiology ; Mission oriented research ; Mycology ; Physiology and metabolism</subject><ispartof>Systematic and applied microbiology, 1997, Vol.20 (1), p.39-49</ispartof><rights>1997 Gustav Fischer Verlag</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-8a0cbe4dfce4de89bb37cc1df558b82230ed7198ead2d26e9f0532b3bd417f0f3</citedby><cites>FETCH-LOGICAL-c367t-8a0cbe4dfce4de89bb37cc1df558b82230ed7198ead2d26e9f0532b3bd417f0f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0723-2020(97)80046-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2721681$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Kock, J.L.F.</creatorcontrib><creatorcontrib>van Vuuren, D. Jansen</creatorcontrib><creatorcontrib>Botha, A.</creatorcontrib><creatorcontrib>van Dyk, M.S.</creatorcontrib><creatorcontrib>Coetzee, D.J.</creatorcontrib><creatorcontrib>Botes, P.J.</creatorcontrib><creatorcontrib>Shaw, N.</creatorcontrib><creatorcontrib>Friend, J.</creatorcontrib><creatorcontrib>Ratledge, C.</creatorcontrib><creatorcontrib>Roberts, A.D.</creatorcontrib><creatorcontrib>Nigam, S.</creatorcontrib><title>The Production of Biologically Active 3-Hydroxy-5,8,11,14-Eicosa Tetraenoic Acid (3-HETE) and Linoleic Acid Metabolites by Dipodascopsis</title><title>Systematic and applied microbiology</title><description>3-Hydroxy-5,8,11,14-eicosatetraenoic acid (3-HETE) is a biologically active compound which has a signal transducing role in human neutrophils. When 12.5 mg/l arachidonic acid (AA) was fed to sexual reproductive cells of
Dipodascopsis uninucleata and
D. tóthii the former transformed approximately 4% of AA to 3-HETE, while the latter produced only trace amounts of 3-HETE. The 3-HETE was only associated with cellular ethanol extracts of both species.
D. uninucleata utilized 69% of the AA after 6h while
D. tóthii utilized 33%. The residual or unused AA was mainly present in the cells of
D. tóthii (96%) and
D. uninucleata (98%) and only small amounts (4% and 2%) in their respective supernatants. Extraction with 80% aqueous ethanol succeeded in removing respectively 92% and 45% of AA present in the cells of
D. tóthii and
D. uninucleata. The poor AA extraction from cells of
D. uninucleata contributed to the presence of AA in the neutral lipid fraction which is insoluble in ethanol. If efficient AA extraction can be implemented, approximately 67% and 31% unused AA can be recovered from
D. tóthii and
D. uninucleata respectively which can be recycled for biotransformation purposes. Although no traces of other 3-hydroxy fatty acids were detected, enzyme extracts from
D. uninucleata metabolised exogenous 18:2 in a manner consistent with the presence of a prostaglandin endoperoxide synthase similar to that in fetal calf blood vessels.</description><subject>18:2-metabolites</subject><subject>3-HETE</subject><subject>Arachidonic acid</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>D. tóthii</subject><subject>D. uninucleata</subject><subject>Dipodascopsis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth, nutrition, metabolism, transports, enzymes. 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Jansen ; Botha, A. ; van Dyk, M.S. ; Coetzee, D.J. ; Botes, P.J. ; Shaw, N. ; Friend, J. ; Ratledge, C. ; Roberts, A.D. ; Nigam, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-8a0cbe4dfce4de89bb37cc1df558b82230ed7198ead2d26e9f0532b3bd417f0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>18:2-metabolites</topic><topic>3-HETE</topic><topic>Arachidonic acid</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>D. tóthii</topic><topic>D. uninucleata</topic><topic>Dipodascopsis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><topic>Mycology</topic><topic>Physiology and metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kock, J.L.F.</creatorcontrib><creatorcontrib>van Vuuren, D. Jansen</creatorcontrib><creatorcontrib>Botha, A.</creatorcontrib><creatorcontrib>van Dyk, M.S.</creatorcontrib><creatorcontrib>Coetzee, D.J.</creatorcontrib><creatorcontrib>Botes, P.J.</creatorcontrib><creatorcontrib>Shaw, N.</creatorcontrib><creatorcontrib>Friend, J.</creatorcontrib><creatorcontrib>Ratledge, C.</creatorcontrib><creatorcontrib>Roberts, A.D.</creatorcontrib><creatorcontrib>Nigam, S.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Systematic and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kock, J.L.F.</au><au>van Vuuren, D. Jansen</au><au>Botha, A.</au><au>van Dyk, M.S.</au><au>Coetzee, D.J.</au><au>Botes, P.J.</au><au>Shaw, N.</au><au>Friend, J.</au><au>Ratledge, C.</au><au>Roberts, A.D.</au><au>Nigam, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Production of Biologically Active 3-Hydroxy-5,8,11,14-Eicosa Tetraenoic Acid (3-HETE) and Linoleic Acid Metabolites by Dipodascopsis</atitle><jtitle>Systematic and applied microbiology</jtitle><date>1997</date><risdate>1997</risdate><volume>20</volume><issue>1</issue><spage>39</spage><epage>49</epage><pages>39-49</pages><issn>0723-2020</issn><eissn>1618-0984</eissn><coden>SAMIDF</coden><abstract>3-Hydroxy-5,8,11,14-eicosatetraenoic acid (3-HETE) is a biologically active compound which has a signal transducing role in human neutrophils. When 12.5 mg/l arachidonic acid (AA) was fed to sexual reproductive cells of
Dipodascopsis uninucleata and
D. tóthii the former transformed approximately 4% of AA to 3-HETE, while the latter produced only trace amounts of 3-HETE. The 3-HETE was only associated with cellular ethanol extracts of both species.
D. uninucleata utilized 69% of the AA after 6h while
D. tóthii utilized 33%. The residual or unused AA was mainly present in the cells of
D. tóthii (96%) and
D. uninucleata (98%) and only small amounts (4% and 2%) in their respective supernatants. Extraction with 80% aqueous ethanol succeeded in removing respectively 92% and 45% of AA present in the cells of
D. tóthii and
D. uninucleata. The poor AA extraction from cells of
D. uninucleata contributed to the presence of AA in the neutral lipid fraction which is insoluble in ethanol. If efficient AA extraction can be implemented, approximately 67% and 31% unused AA can be recovered from
D. tóthii and
D. uninucleata respectively which can be recycled for biotransformation purposes. Although no traces of other 3-hydroxy fatty acids were detected, enzyme extracts from
D. uninucleata metabolised exogenous 18:2 in a manner consistent with the presence of a prostaglandin endoperoxide synthase similar to that in fetal calf blood vessels.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><doi>10.1016/S0723-2020(97)80046-X</doi><tpages>11</tpages></addata></record> |
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| ispartof | Systematic and applied microbiology, 1997, Vol.20 (1), p.39-49 |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | 18:2-metabolites 3-HETE Arachidonic acid Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology D. tóthii D. uninucleata Dipodascopsis Fundamental and applied biological sciences. Psychology Growth, nutrition, metabolism, transports, enzymes. Molecular biology Microbiology Mission oriented research Mycology Physiology and metabolism |
| title | The Production of Biologically Active 3-Hydroxy-5,8,11,14-Eicosa Tetraenoic Acid (3-HETE) and Linoleic Acid Metabolites by Dipodascopsis |
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