Analysis of PCB‐degrading bacteria : physiological aspects

Several aerobic co‐cultures capable of co‐metabolizing polychlorinated biphenyls (PCBs) were acquired by cultivation on biphenyls (BP). The source of micro‐organisms was PCB‐contaminated soil taken from various sites in the Czech Republic. Several bacterial strains (Gram‐negative rods) were isolated...

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Veröffentlicht in:Letters in applied microbiology 1997-05, Vol.24 (5), p.334-336
Hauptverfasser: Pazlarová, J., Demnerová, K., Macková, M., Burkhard, J.
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container_end_page 336
container_issue 5
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container_title Letters in applied microbiology
container_volume 24
creator Pazlarová, J.
Demnerová, K.
Macková, M.
Burkhard, J.
description Several aerobic co‐cultures capable of co‐metabolizing polychlorinated biphenyls (PCBs) were acquired by cultivation on biphenyls (BP). The source of micro‐organisms was PCB‐contaminated soil taken from various sites in the Czech Republic. Several bacterial strains (Gram‐negative rods) were isolated, and their capacity to degrade Delor 103 (a PCB mixture containing di‐ to hexachlorobiphenyls) was analysed. This study was focused on co‐culture 319 and isolate 2. The growth parameters of both those cultures were studied on BP ; for isolate number 2 the specific growth rate μ = 0·122 (h−1) was calculated. The degradation of the individual congeners was estimated and resulted in more than 50% of the degradation of nearly all congeners during a 2‐week experiment. Toxicity of Delor 103 on the vitality of the cells was followed by using viable plate count. The viability of the tested strain was preserved in the 100 times higher Delor 103 concentration compared with conditions in degradation experiments.
doi_str_mv 10.1046/j.1472-765X.1997.00075.x
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source Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection
subjects Aerobiosis
Bacteria - growth & development
Bacteria - isolation & purification
Bacteria - metabolism
Biodegradation of pollutants
Biodegradation, Environmental
Biological and medical sciences
Biotechnology
Environment and pollution
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Kinetics
Polychlorinated Biphenyls - metabolism
Pseudomonas - growth & development
Pseudomonas - isolation & purification
Pseudomonas - metabolism
Soil Microbiology
Soil Pollutants - metabolism
title Analysis of PCB‐degrading bacteria : physiological aspects
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