Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice
Targeted mutagenesis in mice, a powerful tool for the analysis of gene function and human disease, makes extensive use of 129 mouse substrains. Although all are named 129, we document that outcrossing of these substrains, both deliberate and accidental, has lead to extensive genetic variability amon...
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| Veröffentlicht in: | Nature genetics 1997-05, Vol.16 (1), p.19-27 |
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| container_title | Nature genetics |
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| creator | Simpson, Elizabeth M Linder, Carol C Sargent, Evelyn E Davisson, Muriel T Mobraaten, Larry E Sharp, John J |
| description | Targeted mutagenesis in mice, a powerful tool for the analysis of gene function and human disease, makes extensive use of 129 mouse substrains. Although all are named 129, we document that outcrossing of these substrains, both deliberate and accidental, has lead to extensive genetic variability among substrains and embryonic stem cells derived from them. This clearer understanding of 129 substrain variability allows consideration of its negative impact on targeting technology, including: homologous recombination frequencies, preparation of inbred animals, and availability of appropriate controls. Based on these considerations we suggest a number of recommendations for future experimental design. |
| doi_str_mv | 10.1038/ng0597-19 |
| format | Article |
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Although all are named 129, we document that outcrossing of these substrains, both deliberate and accidental, has lead to extensive genetic variability among substrains and embryonic stem cells derived from them. This clearer understanding of 129 substrain variability allows consideration of its negative impact on targeting technology, including: homologous recombination frequencies, preparation of inbred animals, and availability of appropriate controls. 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Biological and molecular evolution ; Graft Rejection - genetics ; Human Genetics ; Male ; Mice ; Mice, Inbred C3H ; Mice, Inbred C57BL ; Mice, Inbred Strains - genetics ; Mice, Mutant Strains ; Mutagenesis ; new-technology ; Polymorphism, Genetic ; Proteins - genetics ; Skin Transplantation ; Stem Cells - cytology ; Vertebrata</subject><ispartof>Nature genetics, 1997-05, Vol.16 (1), p.19-27</ispartof><rights>Springer Nature America, Inc. 1997</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-967a17223000fb8552da839b7b955a9100fe3b1b645c9b491326c13dc767077a3</citedby><cites>FETCH-LOGICAL-c465t-967a17223000fb8552da839b7b955a9100fe3b1b645c9b491326c13dc767077a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/ng0597-19$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/ng0597-19$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27915,27916,41479,42548,51310</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2662207$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9140391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Simpson, Elizabeth M</creatorcontrib><creatorcontrib>Linder, Carol C</creatorcontrib><creatorcontrib>Sargent, Evelyn E</creatorcontrib><creatorcontrib>Davisson, Muriel T</creatorcontrib><creatorcontrib>Mobraaten, Larry E</creatorcontrib><creatorcontrib>Sharp, John J</creatorcontrib><title>Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice</title><title>Nature genetics</title><addtitle>Nat Genet</addtitle><addtitle>Nat Genet</addtitle><description>Targeted mutagenesis in mice, a powerful tool for the analysis of gene function and human disease, makes extensive use of 129 mouse substrains. Although all are named 129, we document that outcrossing of these substrains, both deliberate and accidental, has lead to extensive genetic variability among substrains and embryonic stem cells derived from them. This clearer understanding of 129 substrain variability allows consideration of its negative impact on targeting technology, including: homologous recombination frequencies, preparation of inbred animals, and availability of appropriate controls. Based on these considerations we suggest a number of recommendations for future experimental design.</description><subject>Agriculture</subject><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>Cell Line</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Crosses, Genetic</subject><subject>Embryo, Mammalian - cytology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Function</subject><subject>Genetic Variation</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Graft Rejection - genetics</subject><subject>Human Genetics</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred Strains - genetics</subject><subject>Mice, Mutant Strains</subject><subject>Mutagenesis</subject><subject>new-technology</subject><subject>Polymorphism, Genetic</subject><subject>Proteins - genetics</subject><subject>Skin Transplantation</subject><subject>Stem Cells - cytology</subject><subject>Vertebrata</subject><issn>1061-4036</issn><issn>1546-1718</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU1r3DAQhkVoSPPRQ35AqQ4hkIBbjWVJ1rEsySYQyCW55GLGsmwUbHkryYX8-yp42VNPI-Z9eAceEXIJ7CcwXv_yAxNaFaCPyCmIShagoP6S30xCUTEuv5KzGN8Zg6pi9Qk50ZC3Gk7J29Z6m5yhfzE4TG72FKfZDxRKTePSxhTQ-UjRd9SlSN20m0NCbyzt50AThsEm29FpSTjkqugy4-nkjL0gxz2O0X7bz3Pyen_3snkonp63j5vfT4WppEiFlgpBlSVnjPVtLUTZYc11q1otBGrIW8tbaGUljG4rDbyUBnhnlFRMKeTn5Hrt3YX5z2JjaiYXjR1H9HZeYgOSVSCYzuDNCpowxxhs3-yCmzB8NMCaT4_N6rGBT_b7vnRpJ9sdyL24nF_tc4wGxz5kJS4esFLKsmQqY7crFnPiBxua93kJPuv4780fK-wxLcEeyg6_y_8B71uRgA</recordid><startdate>19970501</startdate><enddate>19970501</enddate><creator>Simpson, Elizabeth M</creator><creator>Linder, Carol C</creator><creator>Sargent, Evelyn E</creator><creator>Davisson, Muriel T</creator><creator>Mobraaten, Larry E</creator><creator>Sharp, John J</creator><general>Nature Publishing Group US</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19970501</creationdate><title>Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice</title><author>Simpson, Elizabeth M ; Linder, Carol C ; Sargent, Evelyn E ; Davisson, Muriel T ; Mobraaten, Larry E ; Sharp, John J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-967a17223000fb8552da839b7b955a9100fe3b1b645c9b491326c13dc767077a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Agriculture</topic><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer Research</topic><topic>Cell Line</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Crosses, Genetic</topic><topic>Embryo, Mammalian - cytology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Function</topic><topic>Genetic Variation</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Graft Rejection - genetics</topic><topic>Human Genetics</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred Strains - genetics</topic><topic>Mice, Mutant Strains</topic><topic>Mutagenesis</topic><topic>new-technology</topic><topic>Polymorphism, Genetic</topic><topic>Proteins - genetics</topic><topic>Skin Transplantation</topic><topic>Stem Cells - cytology</topic><topic>Vertebrata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simpson, Elizabeth M</creatorcontrib><creatorcontrib>Linder, Carol C</creatorcontrib><creatorcontrib>Sargent, Evelyn E</creatorcontrib><creatorcontrib>Davisson, Muriel T</creatorcontrib><creatorcontrib>Mobraaten, Larry E</creatorcontrib><creatorcontrib>Sharp, John J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Nature genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simpson, Elizabeth M</au><au>Linder, Carol C</au><au>Sargent, Evelyn E</au><au>Davisson, Muriel T</au><au>Mobraaten, Larry E</au><au>Sharp, John J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice</atitle><jtitle>Nature genetics</jtitle><stitle>Nat Genet</stitle><addtitle>Nat Genet</addtitle><date>1997-05-01</date><risdate>1997</risdate><volume>16</volume><issue>1</issue><spage>19</spage><epage>27</epage><pages>19-27</pages><issn>1061-4036</issn><eissn>1546-1718</eissn><coden>NGENEC</coden><abstract>Targeted mutagenesis in mice, a powerful tool for the analysis of gene function and human disease, makes extensive use of 129 mouse substrains. Although all are named 129, we document that outcrossing of these substrains, both deliberate and accidental, has lead to extensive genetic variability among substrains and embryonic stem cells derived from them. This clearer understanding of 129 substrain variability allows consideration of its negative impact on targeting technology, including: homologous recombination frequencies, preparation of inbred animals, and availability of appropriate controls. Based on these considerations we suggest a number of recommendations for future experimental design.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>9140391</pmid><doi>10.1038/ng0597-19</doi><tpages>9</tpages></addata></record> |
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| subjects | Agriculture Animal Genetics and Genomics Animals Biological and medical sciences Biomarkers Biomedical and Life Sciences Biomedicine Cancer Research Cell Line Classical genetics, quantitative genetics, hybrids Crosses, Genetic Embryo, Mammalian - cytology Female Fundamental and applied biological sciences. Psychology Gene Function Genetic Variation Genetics of eukaryotes. Biological and molecular evolution Graft Rejection - genetics Human Genetics Male Mice Mice, Inbred C3H Mice, Inbred C57BL Mice, Inbred Strains - genetics Mice, Mutant Strains Mutagenesis new-technology Polymorphism, Genetic Proteins - genetics Skin Transplantation Stem Cells - cytology Vertebrata |
| title | Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice |
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