Expression of colonization factor antigen I fimbriae by enterotoxigenic Escherichia coli; influence of growth conditions and a recombinant positive regulatory gene
Enterotoxigenic Escherichia coli (ETEC) may spontaneously lose the positive regulatory cfaR gene and thereby the capacity to express colonization factor antigen I (CFA/I). A recombinant plasmid harbouring the cfaR gene was transformed into cfaR‐negative mutant ETEC strains. CFA/I expression of wild‐...
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| Veröffentlicht in: | APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 1997-03, Vol.105 (1-6), p.247-254 |
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| container_title | APMIS : acta pathologica, microbiologica et immunologica Scandinavica |
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| creator | HALVORSEN, THOMAS VALVATNE, HÅVARD GREWAL, HARLEEN M. S. GAASTRA, WIM SOMMERFELT, HALVOR |
| description | Enterotoxigenic Escherichia coli (ETEC) may spontaneously lose the positive regulatory cfaR gene and thereby the capacity to express colonization factor antigen I (CFA/I). A recombinant plasmid harbouring the cfaR gene was transformed into cfaR‐negative mutant ETEC strains. CFA/I expression of wild‐type and cfaR‐transformed ETEC cultivated in different liquid media was quantified. At 37°C, a high level of CFA/I expression from wild‐type and cfaR‐transformed strains was observed after growth in CFA broth. Transformation enhanced CFA/I expression only marginally. The transformant cultures showed a considerable variation in CFA/I expression which was paralleled by the proportion of individual bacteria producing CFA/I. This heterogeneity could be explained by a variable tendency to structural CFA/I gene loss among individual cfaR‐transformed bacteria. |
| doi_str_mv | 10.1111/j.1699-0463.1997.tb00565.x |
| format | Article |
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This heterogeneity could be explained by a variable tendency to structural CFA/I gene loss among individual cfaR‐transformed bacteria.</description><identifier>ISSN: 0903-4641</identifier><identifier>EISSN: 1600-0463</identifier><identifier>DOI: 10.1111/j.1699-0463.1997.tb00565.x</identifier><identifier>PMID: 9137521</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; Bacteriology ; Biological and medical sciences ; Child ; colonization factor antigens ; Culture Media ; Diarrhea - microbiology ; Enterotoxigenic Escherichia coli ; Enterotoxins - biosynthesis ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - pathogenicity ; Escherichia coli - physiology ; Escherichia coli Infections - microbiology ; expression ; fimbriae ; Fimbriae Proteins ; Fimbriae, Bacterial - physiology ; Fundamental and applied biological sciences. 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S.</creatorcontrib><creatorcontrib>GAASTRA, WIM</creatorcontrib><creatorcontrib>SOMMERFELT, HALVOR</creatorcontrib><title>Expression of colonization factor antigen I fimbriae by enterotoxigenic Escherichia coli; influence of growth conditions and a recombinant positive regulatory gene</title><title>APMIS : acta pathologica, microbiologica et immunologica Scandinavica</title><addtitle>APMIS</addtitle><description>Enterotoxigenic Escherichia coli (ETEC) may spontaneously lose the positive regulatory cfaR gene and thereby the capacity to express colonization factor antigen I (CFA/I). A recombinant plasmid harbouring the cfaR gene was transformed into cfaR‐negative mutant ETEC strains. CFA/I expression of wild‐type and cfaR‐transformed ETEC cultivated in different liquid media was quantified. At 37°C, a high level of CFA/I expression from wild‐type and cfaR‐transformed strains was observed after growth in CFA broth. Transformation enhanced CFA/I expression only marginally. The transformant cultures showed a considerable variation in CFA/I expression which was paralleled by the proportion of individual bacteria producing CFA/I. This heterogeneity could be explained by a variable tendency to structural CFA/I gene loss among individual cfaR‐transformed bacteria.</description><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>colonization factor antigens</subject><subject>Culture Media</subject><subject>Diarrhea - microbiology</subject><subject>Enterotoxigenic Escherichia coli</subject><subject>Enterotoxins - biosynthesis</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - pathogenicity</subject><subject>Escherichia coli - physiology</subject><subject>Escherichia coli Infections - microbiology</subject><subject>expression</subject><subject>fimbriae</subject><subject>Fimbriae Proteins</subject><subject>Fimbriae, Bacterial - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genes, Regulator</subject><subject>Genetics</subject><subject>growth media</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Mutagenesis</subject><subject>Plasmids</subject><subject>Recombination, Genetic</subject><subject>Transformation, Bacterial</subject><issn>0903-4641</issn><issn>1600-0463</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkV2LEzEUhgdR1rr6E4Qg4t3UfGdGYWFZuh-4risogjchkzlpU6eTmkzd1r_jHzVjS-_NTch53_OcQ96ieEXwlOTzdjklsq5LzCWbkrpW06HBWEgx3T4qJkRi_E96XExwjVnJJSdPi2cpLTEmtJLqpDipCVOCkknxZ7ZdR0jJhx4Fh2zoQu9_m2F8O2OHEJHpBz-HHt0g51dN9AZQs0PQDxDDELaj5i2aJbuA6O3Cm5Hi3yPfu24DvYURPI_hYVhkpW_9CE8Z2yKDItiwanyfh6B1SFn7Bbk433Qmz96hDIfnxRNnugQvDvdp8fVy9uXiurz9dHVzcX5bWi6VKmkLtcJO1ky5RgnSckFr2zSVqRhVLW6osZRWRICtBGecCS4qZ1pcgaycMOy0eLPnrmP4uYE06JVPFrrO9BA2SeefpZhgno3v9kYbQ0oRnF5HvzJxpwnWY0J6qceE9BiDHhPSh4T0Nje_PEzZNCtoj62HSLL--qCbZE3noumtT0cblYwLKbPtbG978B3s_mMBfX7_kXKVAeUe4NMA2yPAxB9aqryK_nZ3pT9X94LdffiuGfsLVqLAwQ</recordid><startdate>19970301</startdate><enddate>19970301</enddate><creator>HALVORSEN, THOMAS</creator><creator>VALVATNE, HÅVARD</creator><creator>GREWAL, HARLEEN M. S.</creator><creator>GAASTRA, WIM</creator><creator>SOMMERFELT, HALVOR</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>19970301</creationdate><title>Expression of colonization factor antigen I fimbriae by enterotoxigenic Escherichia coli; influence of growth conditions and a recombinant positive regulatory gene</title><author>HALVORSEN, THOMAS ; VALVATNE, HÅVARD ; GREWAL, HARLEEN M. S. ; GAASTRA, WIM ; SOMMERFELT, HALVOR</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4677-2de970f6937fb751d4529cbb8a8327d0b2ac22815ec8543435458fad08e68f5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>colonization factor antigens</topic><topic>Culture Media</topic><topic>Diarrhea - microbiology</topic><topic>Enterotoxigenic Escherichia coli</topic><topic>Enterotoxins - biosynthesis</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - pathogenicity</topic><topic>Escherichia coli - physiology</topic><topic>Escherichia coli Infections - microbiology</topic><topic>expression</topic><topic>fimbriae</topic><topic>Fimbriae Proteins</topic><topic>Fimbriae, Bacterial - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genes, Regulator</topic><topic>Genetics</topic><topic>growth media</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Mutagenesis</topic><topic>Plasmids</topic><topic>Recombination, Genetic</topic><topic>Transformation, Bacterial</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HALVORSEN, THOMAS</creatorcontrib><creatorcontrib>VALVATNE, HÅVARD</creatorcontrib><creatorcontrib>GREWAL, HARLEEN M. 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At 37°C, a high level of CFA/I expression from wild‐type and cfaR‐transformed strains was observed after growth in CFA broth. Transformation enhanced CFA/I expression only marginally. The transformant cultures showed a considerable variation in CFA/I expression which was paralleled by the proportion of individual bacteria producing CFA/I. This heterogeneity could be explained by a variable tendency to structural CFA/I gene loss among individual cfaR‐transformed bacteria.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9137521</pmid><doi>10.1111/j.1699-0463.1997.tb00565.x</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0903-4641 |
| ispartof | APMIS : acta pathologica, microbiologica et immunologica Scandinavica, 1997-03, Vol.105 (1-6), p.247-254 |
| issn | 0903-4641 1600-0463 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Bacteriology Biological and medical sciences Child colonization factor antigens Culture Media Diarrhea - microbiology Enterotoxigenic Escherichia coli Enterotoxins - biosynthesis Escherichia coli Escherichia coli - genetics Escherichia coli - pathogenicity Escherichia coli - physiology Escherichia coli Infections - microbiology expression fimbriae Fimbriae Proteins Fimbriae, Bacterial - physiology Fundamental and applied biological sciences. Psychology Genes, Bacterial Genes, Regulator Genetics growth media Humans Microbiology Mutagenesis Plasmids Recombination, Genetic Transformation, Bacterial |
| title | Expression of colonization factor antigen I fimbriae by enterotoxigenic Escherichia coli; influence of growth conditions and a recombinant positive regulatory gene |
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