Isotype switching in anti-immunoglobulin-activated B lymphoblasts: Differential requirements for interleukin 4 and other lymphokines to elicit membrane vs. secreted IgG sub(1)

Isotype switching in anti-immunoglobulin-activated B lymphoblasts: Differential requirements for interleukin 4 and other lymphokines to elicit membrane vs. secreted IgG sub(1)

Anti-immunoglobulin (Ig)-activated B lymphoblasts, prepared by culturing high-density B cells with anti-Ig-Sepharose for 48 h, can be induced to secrete IgM and IgG sub(1) by a mixture of T cell-derived lymphokines containing interleukin (IL) 4. In this study we have examined the conditions required...

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Veröffentlicht in:European journal of immunology 1991-01, Vol.21 (3), p.707-714
Hauptverfasser: Purkerson, J M, Isakson, P C
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container_title European journal of immunology
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creator Purkerson, J M
Isakson, P C
description Anti-immunoglobulin (Ig)-activated B lymphoblasts, prepared by culturing high-density B cells with anti-Ig-Sepharose for 48 h, can be induced to secrete IgM and IgG sub(1) by a mixture of T cell-derived lymphokines containing interleukin (IL) 4. In this study we have examined the conditions required for lymphokine-mediated induction of IgG sub(1) secretion and membrane (m)IgG sub(1) expression in B lymphoblasts. The results suggest that LPS and IL 4 are sufficient to commit B lymphoblasts to mIgG sub(1) expression, but that additional signals provided by T cell-derived lymphokines are required to elicit IgG sub(1) secretion.
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title Isotype switching in anti-immunoglobulin-activated B lymphoblasts: Differential requirements for interleukin 4 and other lymphokines to elicit membrane vs. secreted IgG sub(1)
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