Development of coupling-repulsion-phase SCAR markers diagnostic for the sugar beet Rr1 allele conferring resistance to rhizomania
In sugar beet genotypes with the 'Holly' type of resistance to rhizomania, a disease due to infection of the beet necrotic yellow vein virus (BNYVV), the major gene rr1 is responsible for resistance. Twelve RAPD markers linked to rr1 were selected by BSA and mapped on linkage group IV usin...
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| Veröffentlicht in: | Molecular breeding 1997, Vol.3 (3), p.231-238 |
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| creator | Barzen, E Stahl, R Fuchs, E Borchardt, D.C Salamini, F |
| description | In sugar beet genotypes with the 'Holly' type of resistance to rhizomania, a disease due to infection of the beet necrotic yellow vein virus (BNYVV), the major gene rr1 is responsible for resistance. Twelve RAPD markers linked to rr1 were selected by BSA and mapped on linkage group IV using a segregating population previously analysed by the same group. Markers F61050 and N9600 were tightly linked, respectively in coupling and repulsion, to the Rr1 allele (recombination values of 1.4 cM for both markers). After sequencing the products amplified by F61050 and N9600, new PCR primers were used to generate the two SCAR markers F6 and N9. The simultaneous use of these markers in a PCR reaction allows the correct fingerprinting of rr1 rr1, Rr1 rr1 and Rr1 Rr1 sugar beet plants in populations segregating for the 'Holly' resistance. In a group of sugar beet elite lines containing the 'Holly' type of rhizomania resistance, SCAR F6 is always present whereas the SCAR N9 fragment is absent. Thus, in marker-assisted selection with coupling-repulsion-phase markers, SCAR F6 can be used in combination with N9, or together with any other RAPD marker linked in repulsion to the Rr1 allele. |
| doi_str_mv | 10.1023/a:1009626214058 |
| format | Article |
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Twelve RAPD markers linked to rr1 were selected by BSA and mapped on linkage group IV using a segregating population previously analysed by the same group. Markers F61050 and N9600 were tightly linked, respectively in coupling and repulsion, to the Rr1 allele (recombination values of 1.4 cM for both markers). After sequencing the products amplified by F61050 and N9600, new PCR primers were used to generate the two SCAR markers F6 and N9. The simultaneous use of these markers in a PCR reaction allows the correct fingerprinting of rr1 rr1, Rr1 rr1 and Rr1 Rr1 sugar beet plants in populations segregating for the 'Holly' resistance. In a group of sugar beet elite lines containing the 'Holly' type of rhizomania resistance, SCAR F6 is always present whereas the SCAR N9 fragment is absent. Thus, in marker-assisted selection with coupling-repulsion-phase markers, SCAR F6 can be used in combination with N9, or together with any other RAPD marker linked in repulsion to the Rr1 allele.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1023/a:1009626214058</identifier><language>eng</language><publisher>Dordrecht: Springer Nature B.V</publisher><subject>Alleles ; Beet necrotic yellow vein virus ; Beta vulgaris subsp. vulgaris ; Beta vulgaris vulgaris ; bulked segregant analysis ; chromosome mapping ; Coupling ; Diagnostic systems ; Disease resistance ; Fingerprinting ; genetic markers ; genetic resistance ; genetic techniques and protocols ; Genetics ; Genotypes ; linkage (genetics) ; linkage groups ; loci ; major genes ; Marker-assisted selection ; Markers ; Molecular biology ; Plant biology ; Plant virus diseases ; Primers ; Random amplified polymorphic DNA ; random amplified polymorphic DNA technique ; Recombination ; Rhizomania ; rr1 locus ; segregation ; sequence characterized amplified regions ; Sugar ; Viruses</subject><ispartof>Molecular breeding, 1997, Vol.3 (3), p.231-238</ispartof><rights>Molecular Breeding is a copyright of Springer, (1997). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c263t-40d25fe0d2453bd0035c0ec565876ddee113724e63307822fdd046985af762323</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Barzen, E</creatorcontrib><creatorcontrib>Stahl, R</creatorcontrib><creatorcontrib>Fuchs, E</creatorcontrib><creatorcontrib>Borchardt, D.C</creatorcontrib><creatorcontrib>Salamini, F</creatorcontrib><title>Development of coupling-repulsion-phase SCAR markers diagnostic for the sugar beet Rr1 allele conferring resistance to rhizomania</title><title>Molecular breeding</title><description>In sugar beet genotypes with the 'Holly' type of resistance to rhizomania, a disease due to infection of the beet necrotic yellow vein virus (BNYVV), the major gene rr1 is responsible for resistance. Twelve RAPD markers linked to rr1 were selected by BSA and mapped on linkage group IV using a segregating population previously analysed by the same group. Markers F61050 and N9600 were tightly linked, respectively in coupling and repulsion, to the Rr1 allele (recombination values of 1.4 cM for both markers). After sequencing the products amplified by F61050 and N9600, new PCR primers were used to generate the two SCAR markers F6 and N9. The simultaneous use of these markers in a PCR reaction allows the correct fingerprinting of rr1 rr1, Rr1 rr1 and Rr1 Rr1 sugar beet plants in populations segregating for the 'Holly' resistance. In a group of sugar beet elite lines containing the 'Holly' type of rhizomania resistance, SCAR F6 is always present whereas the SCAR N9 fragment is absent. Thus, in marker-assisted selection with coupling-repulsion-phase markers, SCAR F6 can be used in combination with N9, or together with any other RAPD marker linked in repulsion to the Rr1 allele.</description><subject>Alleles</subject><subject>Beet necrotic yellow vein virus</subject><subject>Beta vulgaris subsp. vulgaris</subject><subject>Beta vulgaris vulgaris</subject><subject>bulked segregant analysis</subject><subject>chromosome mapping</subject><subject>Coupling</subject><subject>Diagnostic systems</subject><subject>Disease resistance</subject><subject>Fingerprinting</subject><subject>genetic markers</subject><subject>genetic resistance</subject><subject>genetic techniques and protocols</subject><subject>Genetics</subject><subject>Genotypes</subject><subject>linkage (genetics)</subject><subject>linkage groups</subject><subject>loci</subject><subject>major genes</subject><subject>Marker-assisted selection</subject><subject>Markers</subject><subject>Molecular biology</subject><subject>Plant biology</subject><subject>Plant virus diseases</subject><subject>Primers</subject><subject>Random amplified polymorphic DNA</subject><subject>random amplified polymorphic DNA technique</subject><subject>Recombination</subject><subject>Rhizomania</subject><subject>rr1 locus</subject><subject>segregation</subject><subject>sequence characterized amplified regions</subject><subject>Sugar</subject><subject>Viruses</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdjktr3DAUhU1poNNJ111GEMjO7dWV9XB2YdI0gYFCHmuj2NceTTSSI9lZdNd_XofpqptzzuLj4xTFVw7fOKD4bi85QK1QIa9Amg_FikuNZa2N-bhsYaAUuhKfis857wFA10qtij_X9EY-jgcKE4s9a-M8eheGMtE4--xiKMedzcQeNlf37GDTC6XMOmeHEPPkWtbHxKYdsTwPNrFnoondJ86s9-Rp0YWeUlqELFF2ebKhJTZFlnbudzzY4OxpcdJbn-nLv14XTzc_Hje35fbXz7vN1bZsUYmprKBD2dOSlRTPHYCQLVArlTRadR0R50JjRUoI0Aax7zqoVG2k7bVCgWJdXBy9Y4qvM-WpObjckvc2UJxzwxVw1FIs4Pl_4D7OKSzfGkRZS4SKv1NnR6q3sbFDcrl5ekDgAtAYXpta_AX1YHjx</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Barzen, E</creator><creator>Stahl, R</creator><creator>Fuchs, E</creator><creator>Borchardt, D.C</creator><creator>Salamini, F</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>1997</creationdate><title>Development of coupling-repulsion-phase SCAR markers diagnostic for the sugar beet Rr1 allele conferring resistance to rhizomania</title><author>Barzen, E ; Stahl, R ; Fuchs, E ; Borchardt, D.C ; Salamini, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c263t-40d25fe0d2453bd0035c0ec565876ddee113724e63307822fdd046985af762323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Alleles</topic><topic>Beet necrotic yellow vein virus</topic><topic>Beta vulgaris subsp. vulgaris</topic><topic>Beta vulgaris vulgaris</topic><topic>bulked segregant analysis</topic><topic>chromosome mapping</topic><topic>Coupling</topic><topic>Diagnostic systems</topic><topic>Disease resistance</topic><topic>Fingerprinting</topic><topic>genetic markers</topic><topic>genetic resistance</topic><topic>genetic techniques and protocols</topic><topic>Genetics</topic><topic>Genotypes</topic><topic>linkage (genetics)</topic><topic>linkage groups</topic><topic>loci</topic><topic>major genes</topic><topic>Marker-assisted selection</topic><topic>Markers</topic><topic>Molecular biology</topic><topic>Plant biology</topic><topic>Plant virus diseases</topic><topic>Primers</topic><topic>Random amplified polymorphic DNA</topic><topic>random amplified polymorphic DNA technique</topic><topic>Recombination</topic><topic>Rhizomania</topic><topic>rr1 locus</topic><topic>segregation</topic><topic>sequence characterized amplified regions</topic><topic>Sugar</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barzen, E</creatorcontrib><creatorcontrib>Stahl, R</creatorcontrib><creatorcontrib>Fuchs, E</creatorcontrib><creatorcontrib>Borchardt, D.C</creatorcontrib><creatorcontrib>Salamini, F</creatorcontrib><collection>AGRIS</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barzen, E</au><au>Stahl, R</au><au>Fuchs, E</au><au>Borchardt, D.C</au><au>Salamini, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of coupling-repulsion-phase SCAR markers diagnostic for the sugar beet Rr1 allele conferring resistance to rhizomania</atitle><jtitle>Molecular breeding</jtitle><date>1997</date><risdate>1997</risdate><volume>3</volume><issue>3</issue><spage>231</spage><epage>238</epage><pages>231-238</pages><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>In sugar beet genotypes with the 'Holly' type of resistance to rhizomania, a disease due to infection of the beet necrotic yellow vein virus (BNYVV), the major gene rr1 is responsible for resistance. Twelve RAPD markers linked to rr1 were selected by BSA and mapped on linkage group IV using a segregating population previously analysed by the same group. Markers F61050 and N9600 were tightly linked, respectively in coupling and repulsion, to the Rr1 allele (recombination values of 1.4 cM for both markers). After sequencing the products amplified by F61050 and N9600, new PCR primers were used to generate the two SCAR markers F6 and N9. The simultaneous use of these markers in a PCR reaction allows the correct fingerprinting of rr1 rr1, Rr1 rr1 and Rr1 Rr1 sugar beet plants in populations segregating for the 'Holly' resistance. In a group of sugar beet elite lines containing the 'Holly' type of rhizomania resistance, SCAR F6 is always present whereas the SCAR N9 fragment is absent. Thus, in marker-assisted selection with coupling-repulsion-phase markers, SCAR F6 can be used in combination with N9, or together with any other RAPD marker linked in repulsion to the Rr1 allele.</abstract><cop>Dordrecht</cop><pub>Springer Nature B.V</pub><doi>10.1023/a:1009626214058</doi><tpages>8</tpages></addata></record> |
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| subjects | Alleles Beet necrotic yellow vein virus Beta vulgaris subsp. vulgaris Beta vulgaris vulgaris bulked segregant analysis chromosome mapping Coupling Diagnostic systems Disease resistance Fingerprinting genetic markers genetic resistance genetic techniques and protocols Genetics Genotypes linkage (genetics) linkage groups loci major genes Marker-assisted selection Markers Molecular biology Plant biology Plant virus diseases Primers Random amplified polymorphic DNA random amplified polymorphic DNA technique Recombination Rhizomania rr1 locus segregation sequence characterized amplified regions Sugar Viruses |
| title | Development of coupling-repulsion-phase SCAR markers diagnostic for the sugar beet Rr1 allele conferring resistance to rhizomania |
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