Determination of mannuronan C-5-epimerase activity in fermentation broth of Azotobacter vinelandii

Azotobacter vinelandii produces extracellular mannuronan C-5-epimerase activity. This activity appears during the biosynthesis of alginate and converts d-mannuronic acid residues into l-guluronic acid in the polymer chain. In order to study the production of mannuronan C-5-epimerase by fermentation...

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Veröffentlicht in:	Enzyme and microbial technology 1997-03, Vol.20 (4), p.308-316
Hauptverfasser:	Ramstad, Marit Valeur, Elingsen, Trond E., Levine, David W.
Format:	Artikel
Sprache:	eng
Schlagworte:	[ 3H]-alginate Azotobacter vinelandii Biological and medical sciences Biotechnology Enzyme engineering Fermentation Fundamental and applied biological sciences. Psychology Mannuronan C-5-epimerase Methods. Procedures. Technologies Production of selected enzymes radioactive assay
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