Cytotoxicity of Organochlorine Pesticides and Lipid Peroxidation in Isolated Rat Hepatocytes
The cytotoxicity and lipid peroxidation of pesticides containing a halogen group were examined in isolated rat hepatocytes. We examined 9 pesticides of 3 different representative chemical families, chlorinated aromatic fungicides (pentachlorophenol (PCP), pentachloronitrobenzene (PCNB), chlorothalon...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 1997/03/15, Vol.20(3), pp.271-274 |
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| creator | SUZUKI, Toshihide KOMATSU, Miho ISONO, Hideo |
| description | The cytotoxicity and lipid peroxidation of pesticides containing a halogen group were examined in isolated rat hepatocytes. We examined 9 pesticides of 3 different representative chemical families, chlorinated aromatic fungicides (pentachlorophenol (PCP), pentachloronitrobenzene (PCNB), chlorothalonil, fthalide), polyhaloalkylated thio fungicides (dichlofluanid, captan) and diphenyl ether herbicide (2, 4-dichlorophenyl 4-nitrophenyl ether (NIP), 4-nitrophenyl 2, 4, 6-trichlorophenyl ether (CNP), chlomethoxynil). The contents of the hydroperoxides in phospholipid, phosphatidylcholine hydroperoxide (PCOOH) and phosphatidylethanolamine hydroperoxide (PEOOH) were determined by the HPLC-chemiluminescence (CL-HPLC) method, which is sensitive and specific for lipid hydroperoxide. Chlorothalonil, dichlofluanid and captan were the most potent cytotoxicants evaluated by lactate dehydrogenase (LDH) leakage. PCP, NIP and CNP exhibited intermediate cytotoxicity. PCNB, fthalide and chlomethoxynil showed low cytotoxicity. The cellular phospholipid hydroperoxide (PCOOH and PEOOH) levels were remarkably increased by chlorothalonil (PCOOH, 23 times and PEOOH, 7 times), dichlofluanid (PCOOH, 523 times and PEOOH, 22 times) and captan (PCOOH, 518 times and PEOOH, 16 times) as compared with the control group. The PCOOH content was slightly increased by PCP (4.8 times) and NIP (6.3 times), whereas the other 4 pesticides did not change the phospholipid hydroperoxide level.Severe cytotoxicity was observed with a remarkable increase of phospholipid hydroperoxide by chlorothalonil, dichlofluanid and captan. |
| doi_str_mv | 10.1248/bpb.20.271 |
| format | Article |
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We examined 9 pesticides of 3 different representative chemical families, chlorinated aromatic fungicides (pentachlorophenol (PCP), pentachloronitrobenzene (PCNB), chlorothalonil, fthalide), polyhaloalkylated thio fungicides (dichlofluanid, captan) and diphenyl ether herbicide (2, 4-dichlorophenyl 4-nitrophenyl ether (NIP), 4-nitrophenyl 2, 4, 6-trichlorophenyl ether (CNP), chlomethoxynil). The contents of the hydroperoxides in phospholipid, phosphatidylcholine hydroperoxide (PCOOH) and phosphatidylethanolamine hydroperoxide (PEOOH) were determined by the HPLC-chemiluminescence (CL-HPLC) method, which is sensitive and specific for lipid hydroperoxide. Chlorothalonil, dichlofluanid and captan were the most potent cytotoxicants evaluated by lactate dehydrogenase (LDH) leakage. PCP, NIP and CNP exhibited intermediate cytotoxicity. PCNB, fthalide and chlomethoxynil showed low cytotoxicity. The cellular phospholipid hydroperoxide (PCOOH and PEOOH) levels were remarkably increased by chlorothalonil (PCOOH, 23 times and PEOOH, 7 times), dichlofluanid (PCOOH, 523 times and PEOOH, 22 times) and captan (PCOOH, 518 times and PEOOH, 16 times) as compared with the control group. The PCOOH content was slightly increased by PCP (4.8 times) and NIP (6.3 times), whereas the other 4 pesticides did not change the phospholipid hydroperoxide level.Severe cytotoxicity was observed with a remarkable increase of phospholipid hydroperoxide by chlorothalonil, dichlofluanid and captan.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.20.271</identifier><identifier>PMID: 9084885</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Animals ; Biological and medical sciences ; Cell Survival - drug effects ; chemiluminescence ; Chromatography, High Pressure Liquid ; Glutathione - metabolism ; hepatocyte ; In Vitro Techniques ; Insecticides - toxicity ; L-Lactate Dehydrogenase - metabolism ; lipid hydroperoxidation ; Lipid Peroxidation - drug effects ; Liver - cytology ; Liver - drug effects ; Liver - metabolism ; Male ; Medical sciences ; Oxidation-Reduction ; pesticide ; Pesticides, fertilizers and other agrochemicals toxicology ; phospholipid hydroperoxide ; Rats ; Rats, Wistar ; Thiobarbituric Acid Reactive Substances - metabolism ; Toxicology</subject><ispartof>Biological and Pharmaceutical Bulletin, 1997/03/15, Vol.20(3), pp.271-274</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>1997 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c658t-515b1d266eb277aaa57e362859b54869dea76a3c90aa50b4511d45a72c0805e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,1878,27906,27907</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2644912$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9084885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SUZUKI, Toshihide</creatorcontrib><creatorcontrib>KOMATSU, Miho</creatorcontrib><creatorcontrib>ISONO, Hideo</creatorcontrib><title>Cytotoxicity of Organochlorine Pesticides and Lipid Peroxidation in Isolated Rat Hepatocytes</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>The cytotoxicity and lipid peroxidation of pesticides containing a halogen group were examined in isolated rat hepatocytes. We examined 9 pesticides of 3 different representative chemical families, chlorinated aromatic fungicides (pentachlorophenol (PCP), pentachloronitrobenzene (PCNB), chlorothalonil, fthalide), polyhaloalkylated thio fungicides (dichlofluanid, captan) and diphenyl ether herbicide (2, 4-dichlorophenyl 4-nitrophenyl ether (NIP), 4-nitrophenyl 2, 4, 6-trichlorophenyl ether (CNP), chlomethoxynil). The contents of the hydroperoxides in phospholipid, phosphatidylcholine hydroperoxide (PCOOH) and phosphatidylethanolamine hydroperoxide (PEOOH) were determined by the HPLC-chemiluminescence (CL-HPLC) method, which is sensitive and specific for lipid hydroperoxide. Chlorothalonil, dichlofluanid and captan were the most potent cytotoxicants evaluated by lactate dehydrogenase (LDH) leakage. PCP, NIP and CNP exhibited intermediate cytotoxicity. PCNB, fthalide and chlomethoxynil showed low cytotoxicity. The cellular phospholipid hydroperoxide (PCOOH and PEOOH) levels were remarkably increased by chlorothalonil (PCOOH, 23 times and PEOOH, 7 times), dichlofluanid (PCOOH, 523 times and PEOOH, 22 times) and captan (PCOOH, 518 times and PEOOH, 16 times) as compared with the control group. The PCOOH content was slightly increased by PCP (4.8 times) and NIP (6.3 times), whereas the other 4 pesticides did not change the phospholipid hydroperoxide level.Severe cytotoxicity was observed with a remarkable increase of phospholipid hydroperoxide by chlorothalonil, dichlofluanid and captan.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Survival - drug effects</subject><subject>chemiluminescence</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Glutathione - metabolism</subject><subject>hepatocyte</subject><subject>In Vitro Techniques</subject><subject>Insecticides - toxicity</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>lipid hydroperoxidation</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Liver - cytology</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Oxidation-Reduction</subject><subject>pesticide</subject><subject>Pesticides, fertilizers and other agrochemicals toxicology</subject><subject>phospholipid hydroperoxide</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Thiobarbituric Acid Reactive Substances - metabolism</subject><subject>Toxicology</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkM-LEzEYhoMoa3f14l0IKB6EqUkmv-YoRXcXCiuyRyF8k6S7KdNkTFKw_70prT14SSDPk-_9eBF6R8mSMq6_jPO4ZGTJFH2BFrTnqhOMipdoQQaqO0mFfo2uS9kSQhRh_RW6GojmWosF-rU61FTTn2BDPeC0wQ_5CWKyz1PKIXr8w5famPMFQ3R4Hebg2mNuPxzUkCIOEd-XNEH1Dv-Eiu_8DDXZQ_XlDXq1gan4t-f7Bj1-__a4uuvWD7f3q6_rzkqhayeoGKljUvqRKQUAQvleMi2GUXAtB-dBSejtQBoiIxeUOi5AMUs0Eb6_QZ9OY-ecfu_bwmYXivXTBNGnfTFUEiJ4T5v44T9xm_Y5ttUM5XygUmmlmvX5ZNmcSsl-Y-YcdpAPhhJz7Nu0vg0jpvXd5Pfnkftx591FPRfc-Mczh2Jh2mSINpSLxuQxlzVtddK2pcKTv3DIrf3JHxPpMPTH1H-HohdqnyEbH_u_a82fJw</recordid><startdate>19970301</startdate><enddate>19970301</enddate><creator>SUZUKI, Toshihide</creator><creator>KOMATSU, Miho</creator><creator>ISONO, Hideo</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19970301</creationdate><title>Cytotoxicity of Organochlorine Pesticides and Lipid Peroxidation in Isolated Rat Hepatocytes</title><author>SUZUKI, Toshihide ; KOMATSU, Miho ; ISONO, Hideo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c658t-515b1d266eb277aaa57e362859b54869dea76a3c90aa50b4511d45a72c0805e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Survival - drug effects</topic><topic>chemiluminescence</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Glutathione - metabolism</topic><topic>hepatocyte</topic><topic>In Vitro Techniques</topic><topic>Insecticides - toxicity</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>lipid hydroperoxidation</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Liver - cytology</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Oxidation-Reduction</topic><topic>pesticide</topic><topic>Pesticides, fertilizers and other agrochemicals toxicology</topic><topic>phospholipid hydroperoxide</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Thiobarbituric Acid Reactive Substances - metabolism</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SUZUKI, Toshihide</creatorcontrib><creatorcontrib>KOMATSU, Miho</creatorcontrib><creatorcontrib>ISONO, Hideo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SUZUKI, Toshihide</au><au>KOMATSU, Miho</au><au>ISONO, Hideo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxicity of Organochlorine Pesticides and Lipid Peroxidation in Isolated Rat Hepatocytes</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>1997-03-01</date><risdate>1997</risdate><volume>20</volume><issue>3</issue><spage>271</spage><epage>274</epage><pages>271-274</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>The cytotoxicity and lipid peroxidation of pesticides containing a halogen group were examined in isolated rat hepatocytes. We examined 9 pesticides of 3 different representative chemical families, chlorinated aromatic fungicides (pentachlorophenol (PCP), pentachloronitrobenzene (PCNB), chlorothalonil, fthalide), polyhaloalkylated thio fungicides (dichlofluanid, captan) and diphenyl ether herbicide (2, 4-dichlorophenyl 4-nitrophenyl ether (NIP), 4-nitrophenyl 2, 4, 6-trichlorophenyl ether (CNP), chlomethoxynil). The contents of the hydroperoxides in phospholipid, phosphatidylcholine hydroperoxide (PCOOH) and phosphatidylethanolamine hydroperoxide (PEOOH) were determined by the HPLC-chemiluminescence (CL-HPLC) method, which is sensitive and specific for lipid hydroperoxide. Chlorothalonil, dichlofluanid and captan were the most potent cytotoxicants evaluated by lactate dehydrogenase (LDH) leakage. PCP, NIP and CNP exhibited intermediate cytotoxicity. PCNB, fthalide and chlomethoxynil showed low cytotoxicity. The cellular phospholipid hydroperoxide (PCOOH and PEOOH) levels were remarkably increased by chlorothalonil (PCOOH, 23 times and PEOOH, 7 times), dichlofluanid (PCOOH, 523 times and PEOOH, 22 times) and captan (PCOOH, 518 times and PEOOH, 16 times) as compared with the control group. The PCOOH content was slightly increased by PCP (4.8 times) and NIP (6.3 times), whereas the other 4 pesticides did not change the phospholipid hydroperoxide level.Severe cytotoxicity was observed with a remarkable increase of phospholipid hydroperoxide by chlorothalonil, dichlofluanid and captan.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>9084885</pmid><doi>10.1248/bpb.20.271</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Cell Survival - drug effects chemiluminescence Chromatography, High Pressure Liquid Glutathione - metabolism hepatocyte In Vitro Techniques Insecticides - toxicity L-Lactate Dehydrogenase - metabolism lipid hydroperoxidation Lipid Peroxidation - drug effects Liver - cytology Liver - drug effects Liver - metabolism Male Medical sciences Oxidation-Reduction pesticide Pesticides, fertilizers and other agrochemicals toxicology phospholipid hydroperoxide Rats Rats, Wistar Thiobarbituric Acid Reactive Substances - metabolism Toxicology |
| title | Cytotoxicity of Organochlorine Pesticides and Lipid Peroxidation in Isolated Rat Hepatocytes |
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