Monoclonal antibodies against Bonamia ostreae (Protozoa: Ascetospora), an intrahaemocytic parasite of flat oyster Ostrea edulis (Mollusca: Bivalvia)
The protozoan Bonamia ostreae (Ascetospora), a parasite of the flat oyster Ostrea edulis was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse m...
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| Veröffentlicht in: | Diseases of aquatic organisms 1991-08, Vol.11 (2), p.135-142 |
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| container_title | Diseases of aquatic organisms |
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| creator | ROGIER, H HERVIO, D BOULO, V CLAVIES, C HERVAUD, E BACHERE, E MIALHE, E GRIZEL, H PAU, B PAOLUCCI, F |
| description | The protozoan Bonamia ostreae (Ascetospora), a parasite of the flat oyster Ostrea edulis was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibodies appeared to react with the same epitopes, at least 4 clearly different epitope specificities were identified. |
| doi_str_mv | 10.3354/dao011135 |
| format | Article |
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Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibodies appeared to react with the same epitopes, at least 4 clearly different epitope specificities were identified.</description><identifier>ISSN: 0177-5103</identifier><identifier>DOI: 10.3354/dao011135</identifier><identifier>CODEN: DAOREO</identifier><language>eng</language><publisher>Oldendorf: Inter-Research</publisher><subject>Animal aquaculture ; Animal productions ; Biological and medical sciences ; Bonamia ostreae ; Fundamental and applied biological sciences. 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Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibodies appeared to react with the same epitopes, at least 4 clearly different epitope specificities were identified.</description><subject>Animal aquaculture</subject><subject>Animal productions</subject><subject>Biological and medical sciences</subject><subject>Bonamia ostreae</subject><subject>Fundamental and applied biological sciences. 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Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibodies appeared to react with the same epitopes, at least 4 clearly different epitope specificities were identified.</abstract><cop>Oldendorf</cop><pub>Inter-Research</pub><doi>10.3354/dao011135</doi><tpages>8</tpages></addata></record> |
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| ispartof | Diseases of aquatic organisms, 1991-08, Vol.11 (2), p.135-142 |
| issn | 0177-5103 |
| language | eng |
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| source | Inter-Research; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animal aquaculture Animal productions Biological and medical sciences Bonamia ostreae Fundamental and applied biological sciences. Psychology Invertebrate aquaculture Marine Mollusca Ostrea edulis |
| title | Monoclonal antibodies against Bonamia ostreae (Protozoa: Ascetospora), an intrahaemocytic parasite of flat oyster Ostrea edulis (Mollusca: Bivalvia) |
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