Expression of Mammalian γ-Aminobutyric Acid Receptors with Distinct Pharmacology in Xenopus Oocytes
γ-Aminobutyric acid (GABA), the major inhibitory neurotransmitter in mammalian brain, is known to interact with two classes of GABA receptors denoted GABAAand GABAB. Using Xenopus oocytes, we compared the electrical and pharmacological properties of GABA receptors expressed by poly(A)+RNA isolated f...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1991-05, Vol.88 (10), p.4318-4322 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4322 |
|---|---|
| container_issue | 10 |
| container_start_page | 4318 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 88 |
| creator | Polenzani, L. Woodward, R. M. Miledi, R. |
| description | γ-Aminobutyric acid (GABA), the major inhibitory neurotransmitter in mammalian brain, is known to interact with two classes of GABA receptors denoted GABAAand GABAB. Using Xenopus oocytes, we compared the electrical and pharmacological properties of GABA receptors expressed by poly(A)+RNA isolated from mammalian brain and retina. RNA from cerebral cortex expressed GABA responses with features characteristic of currents mediated by GABAAreceptors. In contrast, RNA from retina expressed responses mediated by GABAAreceptors and, in addition, GABA responses that were insensitive to the GABAAantagonist bicuculline and the GABABagonist baclofen and showed no modulation by barbiturates or benzodiazepines. The bicuculline/baclofen-insensitive GABA response was a Cl-current that was blocked by picrotoxin but showed little desensitization or outward rectification. Our results suggest that mammalian retina contains RNAs encoding GABA receptors with distinct pharmacology. |
| doi_str_mv | 10.1073/pnas.88.10.4318 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_15991006</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>2357035</jstor_id><sourcerecordid>2357035</sourcerecordid><originalsourceid>FETCH-LOGICAL-c493t-fd77cdad7484201ae934cc35ff255e98f06cd5edcb41535d9ea4de8561b8c1c93</originalsourceid><addsrcrecordid>eNqFkc1u1DAURi0EKkNhzQaQV7DK9HpsJ7bEZlTKj1RUhEBiZ3kcp-MqsYPtQOe5eA-eCUczFLqBlXX1ne_qWgehxwSWBBp6MnqdlkKUYckoEXfQgoAkVc0k3EULgFVTCbZi99GDlK4AQHIBR-iINCAbRhaoPbseo03JBY9Dh9_rYdC90x7__FGtB-fDZsq76AxeG9fij9bYMYeY8HeXt_iVS9l5k_GHrY6DNqEPlzvsPP5ifRinhC-C2WWbHqJ7ne6TfXR4j9Hn12efTt9W5xdv3p2uzyvDJM1V1zaNaXXbsHIyEG0lZcZQ3nUrzq0UHdSm5bY1G0Y45a20mrVW8JpshCFG0mP0cr93nDZD4azPUfdqjG7QcaeCdup24t1WXYZvipOaQ6k_P9Rj-DrZlNXgkrF9r70NU1ICeENFTf4LEi4lAagLeLIHTQwpRdvd3EJAzf7U7E8JMc-zv9J4-vcX_vB7YSV_dsjn4u_01oIX_wRUN_V9tte5kE_25FUqSm_QFeUNUE5_ARDAvBM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15991006</pqid></control><display><type>article</type><title>Expression of Mammalian γ-Aminobutyric Acid Receptors with Distinct Pharmacology in Xenopus Oocytes</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Polenzani, L. ; Woodward, R. M. ; Miledi, R.</creator><creatorcontrib>Polenzani, L. ; Woodward, R. M. ; Miledi, R.</creatorcontrib><description>γ-Aminobutyric acid (GABA), the major inhibitory neurotransmitter in mammalian brain, is known to interact with two classes of GABA receptors denoted GABAAand GABAB. Using Xenopus oocytes, we compared the electrical and pharmacological properties of GABA receptors expressed by poly(A)+RNA isolated from mammalian brain and retina. RNA from cerebral cortex expressed GABA responses with features characteristic of currents mediated by GABAAreceptors. In contrast, RNA from retina expressed responses mediated by GABAAreceptors and, in addition, GABA responses that were insensitive to the GABAAantagonist bicuculline and the GABABagonist baclofen and showed no modulation by barbiturates or benzodiazepines. The bicuculline/baclofen-insensitive GABA response was a Cl-current that was blocked by picrotoxin but showed little desensitization or outward rectification. Our results suggest that mammalian retina contains RNAs encoding GABA receptors with distinct pharmacology.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.88.10.4318</identifier><identifier>PMID: 1709741</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Baclofen - pharmacology ; Bicuculline - pharmacology ; Cattle ; Cerebral Cortex - chemistry ; Electric Conductivity ; gamma-Aminobutyric Acid - pharmacology ; Gene Expression ; Membrane Potentials ; Oocytes - physiology ; Pentobarbital - pharmacology ; Picrotoxin - pharmacology ; Poly A - genetics ; Rats ; Receptors, GABA-A - drug effects ; Receptors, GABA-A - genetics ; Receptors, GABA-A - physiology ; Retina - chemistry ; RNA - genetics ; RNA, Messenger ; Transfection ; Xenopus ; Xenopus laevis</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1991-05, Vol.88 (10), p.4318-4322</ispartof><rights>Copyright 1991 The National Academy of Sciences of the United States of America</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-fd77cdad7484201ae934cc35ff255e98f06cd5edcb41535d9ea4de8561b8c1c93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/88/10.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2357035$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2357035$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27922,27923,53789,53791,58015,58248</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1709741$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Polenzani, L.</creatorcontrib><creatorcontrib>Woodward, R. M.</creatorcontrib><creatorcontrib>Miledi, R.</creatorcontrib><title>Expression of Mammalian γ-Aminobutyric Acid Receptors with Distinct Pharmacology in Xenopus Oocytes</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>γ-Aminobutyric acid (GABA), the major inhibitory neurotransmitter in mammalian brain, is known to interact with two classes of GABA receptors denoted GABAAand GABAB. Using Xenopus oocytes, we compared the electrical and pharmacological properties of GABA receptors expressed by poly(A)+RNA isolated from mammalian brain and retina. RNA from cerebral cortex expressed GABA responses with features characteristic of currents mediated by GABAAreceptors. In contrast, RNA from retina expressed responses mediated by GABAAreceptors and, in addition, GABA responses that were insensitive to the GABAAantagonist bicuculline and the GABABagonist baclofen and showed no modulation by barbiturates or benzodiazepines. The bicuculline/baclofen-insensitive GABA response was a Cl-current that was blocked by picrotoxin but showed little desensitization or outward rectification. Our results suggest that mammalian retina contains RNAs encoding GABA receptors with distinct pharmacology.</description><subject>Animals</subject><subject>Baclofen - pharmacology</subject><subject>Bicuculline - pharmacology</subject><subject>Cattle</subject><subject>Cerebral Cortex - chemistry</subject><subject>Electric Conductivity</subject><subject>gamma-Aminobutyric Acid - pharmacology</subject><subject>Gene Expression</subject><subject>Membrane Potentials</subject><subject>Oocytes - physiology</subject><subject>Pentobarbital - pharmacology</subject><subject>Picrotoxin - pharmacology</subject><subject>Poly A - genetics</subject><subject>Rats</subject><subject>Receptors, GABA-A - drug effects</subject><subject>Receptors, GABA-A - genetics</subject><subject>Receptors, GABA-A - physiology</subject><subject>Retina - chemistry</subject><subject>RNA - genetics</subject><subject>RNA, Messenger</subject><subject>Transfection</subject><subject>Xenopus</subject><subject>Xenopus laevis</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURi0EKkNhzQaQV7DK9HpsJ7bEZlTKj1RUhEBiZ3kcp-MqsYPtQOe5eA-eCUczFLqBlXX1ne_qWgehxwSWBBp6MnqdlkKUYckoEXfQgoAkVc0k3EULgFVTCbZi99GDlK4AQHIBR-iINCAbRhaoPbseo03JBY9Dh9_rYdC90x7__FGtB-fDZsq76AxeG9fij9bYMYeY8HeXt_iVS9l5k_GHrY6DNqEPlzvsPP5ifRinhC-C2WWbHqJ7ne6TfXR4j9Hn12efTt9W5xdv3p2uzyvDJM1V1zaNaXXbsHIyEG0lZcZQ3nUrzq0UHdSm5bY1G0Y45a20mrVW8JpshCFG0mP0cr93nDZD4azPUfdqjG7QcaeCdup24t1WXYZvipOaQ6k_P9Rj-DrZlNXgkrF9r70NU1ICeENFTf4LEi4lAagLeLIHTQwpRdvd3EJAzf7U7E8JMc-zv9J4-vcX_vB7YSV_dsjn4u_01oIX_wRUN_V9tte5kE_25FUqSm_QFeUNUE5_ARDAvBM</recordid><startdate>19910515</startdate><enddate>19910515</enddate><creator>Polenzani, L.</creator><creator>Woodward, R. M.</creator><creator>Miledi, R.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19910515</creationdate><title>Expression of Mammalian γ-Aminobutyric Acid Receptors with Distinct Pharmacology in Xenopus Oocytes</title><author>Polenzani, L. ; Woodward, R. M. ; Miledi, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-fd77cdad7484201ae934cc35ff255e98f06cd5edcb41535d9ea4de8561b8c1c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Baclofen - pharmacology</topic><topic>Bicuculline - pharmacology</topic><topic>Cattle</topic><topic>Cerebral Cortex - chemistry</topic><topic>Electric Conductivity</topic><topic>gamma-Aminobutyric Acid - pharmacology</topic><topic>Gene Expression</topic><topic>Membrane Potentials</topic><topic>Oocytes - physiology</topic><topic>Pentobarbital - pharmacology</topic><topic>Picrotoxin - pharmacology</topic><topic>Poly A - genetics</topic><topic>Rats</topic><topic>Receptors, GABA-A - drug effects</topic><topic>Receptors, GABA-A - genetics</topic><topic>Receptors, GABA-A - physiology</topic><topic>Retina - chemistry</topic><topic>RNA - genetics</topic><topic>RNA, Messenger</topic><topic>Transfection</topic><topic>Xenopus</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Polenzani, L.</creatorcontrib><creatorcontrib>Woodward, R. M.</creatorcontrib><creatorcontrib>Miledi, R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Polenzani, L.</au><au>Woodward, R. M.</au><au>Miledi, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Mammalian γ-Aminobutyric Acid Receptors with Distinct Pharmacology in Xenopus Oocytes</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1991-05-15</date><risdate>1991</risdate><volume>88</volume><issue>10</issue><spage>4318</spage><epage>4322</epage><pages>4318-4322</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>γ-Aminobutyric acid (GABA), the major inhibitory neurotransmitter in mammalian brain, is known to interact with two classes of GABA receptors denoted GABAAand GABAB. Using Xenopus oocytes, we compared the electrical and pharmacological properties of GABA receptors expressed by poly(A)+RNA isolated from mammalian brain and retina. RNA from cerebral cortex expressed GABA responses with features characteristic of currents mediated by GABAAreceptors. In contrast, RNA from retina expressed responses mediated by GABAAreceptors and, in addition, GABA responses that were insensitive to the GABAAantagonist bicuculline and the GABABagonist baclofen and showed no modulation by barbiturates or benzodiazepines. The bicuculline/baclofen-insensitive GABA response was a Cl-current that was blocked by picrotoxin but showed little desensitization or outward rectification. Our results suggest that mammalian retina contains RNAs encoding GABA receptors with distinct pharmacology.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1709741</pmid><doi>10.1073/pnas.88.10.4318</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1991-05, Vol.88 (10), p.4318-4322 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15991006 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Baclofen - pharmacology Bicuculline - pharmacology Cattle Cerebral Cortex - chemistry Electric Conductivity gamma-Aminobutyric Acid - pharmacology Gene Expression Membrane Potentials Oocytes - physiology Pentobarbital - pharmacology Picrotoxin - pharmacology Poly A - genetics Rats Receptors, GABA-A - drug effects Receptors, GABA-A - genetics Receptors, GABA-A - physiology Retina - chemistry RNA - genetics RNA, Messenger Transfection Xenopus Xenopus laevis |
| title | Expression of Mammalian γ-Aminobutyric Acid Receptors with Distinct Pharmacology in Xenopus Oocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T08%3A27%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20Mammalian%20%CE%B3-Aminobutyric%20Acid%20Receptors%20with%20Distinct%20Pharmacology%20in%20Xenopus%20Oocytes&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Polenzani,%20L.&rft.date=1991-05-15&rft.volume=88&rft.issue=10&rft.spage=4318&rft.epage=4322&rft.pages=4318-4322&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.88.10.4318&rft_dat=%3Cjstor_proqu%3E2357035%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15991006&rft_id=info:pmid/1709741&rft_jstor_id=2357035&rfr_iscdi=true |