Effects of Oxygen Concentration on the Expression of Cytochrome c and Cytochrome c Oxidase Genes in Yeast
Oxygen is an important environmental regulator for the transcription of several genes in Saccharomyces cerevisiae , but it is not yet clear how this yeast or other eukaryotes actually sense oxygen. To begin to address this we have examined the effects of oxygen concentration on the expression of sev...
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| Veröffentlicht in: | The Journal of biological chemistry 1997-06, Vol.272 (23), p.14705-14712 |
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| creator | Burke, P V Raitt, D C Allen, L A Kellogg, E A Poyton, R O |
| description | Oxygen is an important environmental regulator for the transcription of several genes in Saccharomyces cerevisiae , but it is not yet clear how this yeast or other eukaryotes actually sense oxygen. To begin to address this we have examined
the effects of oxygen concentration on the expression of several nuclear genes ( CYC1 , CYC7 , COX4 , COX5a , COX5b , COX6 , COX7 , COX8 , and COX9 ) for proteins of the terminal portion of the respiratory chain. COX5b and CYC7 are hypoxic genes; the rest are aerobic genes. We have found that the level of expression of these genes is determined by
oxygen concentration per se and not merely the presence or absence of oxygen and that each of these genes has a low oxygen threshold (0.5â1 μ m O 2 ) for expression. For some aerobic genes ( COX4 , COX5a , COX7 , COX8 , and COX9 ) there is a gradual decline in expression between 200 μ m O 2 (air) and their oxygen threshold. Below this threshold expression drops precipitously. For others ( COX5a and CYC1 ) the level of expression is nearly constant between 200 μ m O 2 and their threshold and then drops off. The hypoxic genes COX5b and CYC7 are not expressed until the oxygen concentration is below 0.5 μ m O 2 . These studies have also revealed that COX5a and CYC1 , the genes for the aerobic isoforms of cytochrome c oxidase subunit V and cytochrome c , and COX5b and CYC7 , the genes for the hypoxic isoforms of cytochrome c oxidase subunit V and cytochrome c , are coexpressed at a variety of oxygen concentrations and switch on or off at extremely low oxygen concentrations. By shifting
cells from one oxygen concentration to another we have found that aerobic genes are induced faster than hypoxic genes and
that transcripts from both types of gene are turned over quickly. These findings have important implications for cytochrome
c oxidase function and biogenesis and for models of oxygen sensing in yeast. |
| doi_str_mv | 10.1074/jbc.272.23.14705 |
| format | Article |
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the effects of oxygen concentration on the expression of several nuclear genes ( CYC1 , CYC7 , COX4 , COX5a , COX5b , COX6 , COX7 , COX8 , and COX9 ) for proteins of the terminal portion of the respiratory chain. COX5b and CYC7 are hypoxic genes; the rest are aerobic genes. We have found that the level of expression of these genes is determined by
oxygen concentration per se and not merely the presence or absence of oxygen and that each of these genes has a low oxygen threshold (0.5â1 μ m O 2 ) for expression. For some aerobic genes ( COX4 , COX5a , COX7 , COX8 , and COX9 ) there is a gradual decline in expression between 200 μ m O 2 (air) and their oxygen threshold. Below this threshold expression drops precipitously. For others ( COX5a and CYC1 ) the level of expression is nearly constant between 200 μ m O 2 and their threshold and then drops off. The hypoxic genes COX5b and CYC7 are not expressed until the oxygen concentration is below 0.5 μ m O 2 . These studies have also revealed that COX5a and CYC1 , the genes for the aerobic isoforms of cytochrome c oxidase subunit V and cytochrome c , and COX5b and CYC7 , the genes for the hypoxic isoforms of cytochrome c oxidase subunit V and cytochrome c , are coexpressed at a variety of oxygen concentrations and switch on or off at extremely low oxygen concentrations. By shifting
cells from one oxygen concentration to another we have found that aerobic genes are induced faster than hypoxic genes and
that transcripts from both types of gene are turned over quickly. These findings have important implications for cytochrome
c oxidase function and biogenesis and for models of oxygen sensing in yeast.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.272.23.14705</identifier><identifier>PMID: 9169434</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Aerobiosis ; Anaerobiosis ; Cell Nucleus - metabolism ; Cytochrome c Group - biosynthesis ; Electron Transport Complex IV - biosynthesis ; Enzyme Induction ; Gene Expression Regulation, Fungal - drug effects ; Genes, Fungal ; Kinetics ; Oxygen - pharmacology ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; Saccharomyces cerevisiae - metabolism ; Transcription, Genetic</subject><ispartof>The Journal of biological chemistry, 1997-06, Vol.272 (23), p.14705-14712</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-c61b6b8369ff7fd55b63226db0f0aad7183b281c592768974899de97a2ec1c953</citedby><cites>FETCH-LOGICAL-c491t-c61b6b8369ff7fd55b63226db0f0aad7183b281c592768974899de97a2ec1c953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9169434$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burke, P V</creatorcontrib><creatorcontrib>Raitt, D C</creatorcontrib><creatorcontrib>Allen, L A</creatorcontrib><creatorcontrib>Kellogg, E A</creatorcontrib><creatorcontrib>Poyton, R O</creatorcontrib><title>Effects of Oxygen Concentration on the Expression of Cytochrome c and Cytochrome c Oxidase Genes in Yeast</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Oxygen is an important environmental regulator for the transcription of several genes in Saccharomyces cerevisiae , but it is not yet clear how this yeast or other eukaryotes actually sense oxygen. To begin to address this we have examined
the effects of oxygen concentration on the expression of several nuclear genes ( CYC1 , CYC7 , COX4 , COX5a , COX5b , COX6 , COX7 , COX8 , and COX9 ) for proteins of the terminal portion of the respiratory chain. COX5b and CYC7 are hypoxic genes; the rest are aerobic genes. We have found that the level of expression of these genes is determined by
oxygen concentration per se and not merely the presence or absence of oxygen and that each of these genes has a low oxygen threshold (0.5â1 μ m O 2 ) for expression. For some aerobic genes ( COX4 , COX5a , COX7 , COX8 , and COX9 ) there is a gradual decline in expression between 200 μ m O 2 (air) and their oxygen threshold. Below this threshold expression drops precipitously. For others ( COX5a and CYC1 ) the level of expression is nearly constant between 200 μ m O 2 and their threshold and then drops off. The hypoxic genes COX5b and CYC7 are not expressed until the oxygen concentration is below 0.5 μ m O 2 . These studies have also revealed that COX5a and CYC1 , the genes for the aerobic isoforms of cytochrome c oxidase subunit V and cytochrome c , and COX5b and CYC7 , the genes for the hypoxic isoforms of cytochrome c oxidase subunit V and cytochrome c , are coexpressed at a variety of oxygen concentrations and switch on or off at extremely low oxygen concentrations. By shifting
cells from one oxygen concentration to another we have found that aerobic genes are induced faster than hypoxic genes and
that transcripts from both types of gene are turned over quickly. These findings have important implications for cytochrome
c oxidase function and biogenesis and for models of oxygen sensing in yeast.</description><subject>Aerobiosis</subject><subject>Anaerobiosis</subject><subject>Cell Nucleus - metabolism</subject><subject>Cytochrome c Group - biosynthesis</subject><subject>Electron Transport Complex IV - biosynthesis</subject><subject>Enzyme Induction</subject><subject>Gene Expression Regulation, Fungal - drug effects</subject><subject>Genes, Fungal</subject><subject>Kinetics</subject><subject>Oxygen - pharmacology</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Transcription, Genetic</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1Lw0AQxRdRaq3evQh7EG-J-5FsskcptQqFXhT0tGw2s82WJqm7Kbb_vekHQoeBgZn3HsMPoXtKYkqy5HlZmJhlLGY8pklG0gs0pCTnEU_p1yUaEsJoJFmaX6ObEJakr0TSARpIKmTCkyFyE2vBdAG3Fs-3uwU0eNw2BprO6861De67qwBPtmsPIRw2Fo93XWsq39aADdZNeb6Yb12pA-ApNBCwa_A36NDdoiurVwHuTnOEPl8nH-O3aDafvo9fZpHpX-siI2ghipwLaW1myzQtBGdMlAWxROsyozkvWE5NKlkmcpkluZQlyEwzMNTIlI_Q0zF37dufDYRO1S4YWK10A-0mKJrKXHCxF5Kj0Pg2BA9Wrb2rtd8pStSerurpqp6uYlwd6PaWh1P2pqih_DeccPb3x-O9covq13lQheu5QH0e8weojYGy</recordid><startdate>19970606</startdate><enddate>19970606</enddate><creator>Burke, P V</creator><creator>Raitt, D C</creator><creator>Allen, L A</creator><creator>Kellogg, E A</creator><creator>Poyton, R O</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope></search><sort><creationdate>19970606</creationdate><title>Effects of Oxygen Concentration on the Expression of Cytochrome c and Cytochrome c Oxidase Genes in Yeast</title><author>Burke, P V ; Raitt, D C ; Allen, L A ; Kellogg, E A ; Poyton, R O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-c61b6b8369ff7fd55b63226db0f0aad7183b281c592768974899de97a2ec1c953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Aerobiosis</topic><topic>Anaerobiosis</topic><topic>Cell Nucleus - metabolism</topic><topic>Cytochrome c Group - biosynthesis</topic><topic>Electron Transport Complex IV - biosynthesis</topic><topic>Enzyme Induction</topic><topic>Gene Expression Regulation, Fungal - drug effects</topic><topic>Genes, Fungal</topic><topic>Kinetics</topic><topic>Oxygen - pharmacology</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - growth & development</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burke, P V</creatorcontrib><creatorcontrib>Raitt, D C</creatorcontrib><creatorcontrib>Allen, L A</creatorcontrib><creatorcontrib>Kellogg, E A</creatorcontrib><creatorcontrib>Poyton, R O</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burke, P V</au><au>Raitt, D C</au><au>Allen, L A</au><au>Kellogg, E A</au><au>Poyton, R O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Oxygen Concentration on the Expression of Cytochrome c and Cytochrome c Oxidase Genes in Yeast</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1997-06-06</date><risdate>1997</risdate><volume>272</volume><issue>23</issue><spage>14705</spage><epage>14712</epage><pages>14705-14712</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Oxygen is an important environmental regulator for the transcription of several genes in Saccharomyces cerevisiae , but it is not yet clear how this yeast or other eukaryotes actually sense oxygen. To begin to address this we have examined
the effects of oxygen concentration on the expression of several nuclear genes ( CYC1 , CYC7 , COX4 , COX5a , COX5b , COX6 , COX7 , COX8 , and COX9 ) for proteins of the terminal portion of the respiratory chain. COX5b and CYC7 are hypoxic genes; the rest are aerobic genes. We have found that the level of expression of these genes is determined by
oxygen concentration per se and not merely the presence or absence of oxygen and that each of these genes has a low oxygen threshold (0.5â1 μ m O 2 ) for expression. For some aerobic genes ( COX4 , COX5a , COX7 , COX8 , and COX9 ) there is a gradual decline in expression between 200 μ m O 2 (air) and their oxygen threshold. Below this threshold expression drops precipitously. For others ( COX5a and CYC1 ) the level of expression is nearly constant between 200 μ m O 2 and their threshold and then drops off. The hypoxic genes COX5b and CYC7 are not expressed until the oxygen concentration is below 0.5 μ m O 2 . These studies have also revealed that COX5a and CYC1 , the genes for the aerobic isoforms of cytochrome c oxidase subunit V and cytochrome c , and COX5b and CYC7 , the genes for the hypoxic isoforms of cytochrome c oxidase subunit V and cytochrome c , are coexpressed at a variety of oxygen concentrations and switch on or off at extremely low oxygen concentrations. By shifting
cells from one oxygen concentration to another we have found that aerobic genes are induced faster than hypoxic genes and
that transcripts from both types of gene are turned over quickly. These findings have important implications for cytochrome
c oxidase function and biogenesis and for models of oxygen sensing in yeast.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>9169434</pmid><doi>10.1074/jbc.272.23.14705</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aerobiosis Anaerobiosis Cell Nucleus - metabolism Cytochrome c Group - biosynthesis Electron Transport Complex IV - biosynthesis Enzyme Induction Gene Expression Regulation, Fungal - drug effects Genes, Fungal Kinetics Oxygen - pharmacology Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism Transcription, Genetic |
| title | Effects of Oxygen Concentration on the Expression of Cytochrome c and Cytochrome c Oxidase Genes in Yeast |
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