Site-directed mutagenesis of ricin A chain Trp 211 to Phe

Oligonucleotide‐directed site‐specific mutagenesis has been used to make a conservative change in the putative active site of the catalytic subunit of the toxin ricin. The substitution of phenylalanine for tryptophan at position 211 of the A chain reduces but does not abolish catalytic activity, as...

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Veröffentlicht in:	International Journal of Peptide and Protein Research 1990-04, Vol.35 (4), p.365-366
Hauptverfasser:	BRADLEY, JANICE L., McGUIRE, PETER M.
Format:	Artikel
Sprache:	eng
Schlagworte:	active site Amino Acid Sequence Analytical, structural and metabolic biochemistry Base Sequence Binding Sites Biological and medical sciences Fundamental and applied biological sciences. Psychology Miscellaneous Molecular Sequence Data mutagenesis Mutation Phenylalanine Proteins Recombinant Proteins - genetics Recombinant Proteins - metabolism Ricin - genetics Ricin - metabolism ricin A chain site-directed mutagenesis Tryptophan
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Beschreibung
Zusammenfassung:	Oligonucleotide‐directed site‐specific mutagenesis has been used to make a conservative change in the putative active site of the catalytic subunit of the toxin ricin. The substitution of phenylalanine for tryptophan at position 211 of the A chain reduces but does not abolish catalytic activity, as assayed by inhibition of cell‐free protein synthesis.
ISSN:	0367-8377 1399-3011
DOI:	10.1111/j.1399-3011.1990.tb00062.x