Heat-Shock Protein Expression in Mytilus californianus: Acclimatization (Seasonal and Tidal-Height Comparisons) and Acclimation Effects
Heat-shock protein (hsp) expression was examined in gill of field-acclimatized and laboratory-acclimated mussels (Mytilus californianus) from the Oregon coast. Endogenous levels of heat-shock proteins in the 70-kDa class (hsp70 isoforms) and profiles of induction temperature for newly synthesized hs...
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Veröffentlicht in: | The Biological bulletin 1997-04, Vol.192 (2), p.309-320 |
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description | Heat-shock protein (hsp) expression was examined in gill of field-acclimatized and laboratory-acclimated mussels (Mytilus californianus) from the Oregon coast. Endogenous levels of heat-shock proteins in the 70-kDa class (hsp70 isoforms) and profiles of induction temperature for newly synthesized hsp70 were measured in freshly field-collected specimens as functions of location height in the intertidal and season, and in mussels after 7 weeks of laboratory thermal acclimation. There were significant differences in endogenous levels of hsp70 as functions of season and collection height. Strong induction of new hsp70 synthesis occurred at body temperatures within the range measured in field specimens. Profiles of hsp70 thermal induction varied significantly with season, but not with height of collection. In contrast to the large differences in hsp70 expression between winter- and summer-acclimatized mussels, no differences related to temperature occurred in the differently acclimated mussels. The differences found between the effects of field acclimatization and laboratory thermal acclimation suggest that the stress response is modulated by environmental factors in addition to body temperature. Thus, caution is required in extrapolating from laboratory acclimation studies to acclimatization effects in field populations. The seasonal and tidal-height variations in the heat-shock response are discussed in the context of energy costs of protein turnover. |
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Endogenous levels of heat-shock proteins in the 70-kDa class (hsp70 isoforms) and profiles of induction temperature for newly synthesized hsp70 were measured in freshly field-collected specimens as functions of location height in the intertidal and season, and in mussels after 7 weeks of laboratory thermal acclimation. There were significant differences in endogenous levels of hsp70 as functions of season and collection height. Strong induction of new hsp70 synthesis occurred at body temperatures within the range measured in field specimens. Profiles of hsp70 thermal induction varied significantly with season, but not with height of collection. In contrast to the large differences in hsp70 expression between winter- and summer-acclimatized mussels, no differences related to temperature occurred in the differently acclimated mussels. The differences found between the effects of field acclimatization and laboratory thermal acclimation suggest that the stress response is modulated by environmental factors in addition to body temperature. Thus, caution is required in extrapolating from laboratory acclimation studies to acclimatization effects in field populations. The seasonal and tidal-height variations in the heat-shock response are discussed in the context of energy costs of protein turnover.</description><identifier>ISSN: 0006-3185</identifier><identifier>EISSN: 1939-8697</identifier><identifier>DOI: 10.2307/1542724</identifier><identifier>PMID: 28581871</identifier><language>eng</language><publisher>United States: Marine Biological Laboratory</publisher><subject>Acclimatization ; Cellular biology ; Gels ; Heat shock proteins ; Heat shock response ; High temperature ; Inductive reasoning ; Marine ; Mollusks ; Mussels ; Mytilus californianus ; Physiology ; Protein isoforms ; Protein synthesis ; Proteins ; Sea water</subject><ispartof>The Biological bulletin, 1997-04, Vol.192 (2), p.309-320</ispartof><rights>Copyright 1997 The Marine Biological Laboratory</rights><rights>Copyright © 1997 by Marine Biological Laboratory</rights><rights>Copyright Marine Biological Laboratory Apr 1997</rights><rights>In copyright. Digitized with the permission of the rights holder. http://creativecommons.org/licenses/by-nc-sa/3.0</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c595t-698143906578a2906b6c41663001134ea042b4f04f4ab2b74f8f95b1208a39603</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1542724$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1542724$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28581871$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roberts, Deirdre A.</creatorcontrib><creatorcontrib>Hofmann, Gretchen E.</creatorcontrib><creatorcontrib>Somero, George N.</creatorcontrib><title>Heat-Shock Protein Expression in Mytilus californianus: Acclimatization (Seasonal and Tidal-Height Comparisons) and Acclimation Effects</title><title>The Biological bulletin</title><addtitle>Biol Bull</addtitle><description>Heat-shock protein (hsp) expression was examined in gill of field-acclimatized and laboratory-acclimated mussels (Mytilus californianus) from the Oregon coast. Endogenous levels of heat-shock proteins in the 70-kDa class (hsp70 isoforms) and profiles of induction temperature for newly synthesized hsp70 were measured in freshly field-collected specimens as functions of location height in the intertidal and season, and in mussels after 7 weeks of laboratory thermal acclimation. There were significant differences in endogenous levels of hsp70 as functions of season and collection height. Strong induction of new hsp70 synthesis occurred at body temperatures within the range measured in field specimens. Profiles of hsp70 thermal induction varied significantly with season, but not with height of collection. In contrast to the large differences in hsp70 expression between winter- and summer-acclimatized mussels, no differences related to temperature occurred in the differently acclimated mussels. The differences found between the effects of field acclimatization and laboratory thermal acclimation suggest that the stress response is modulated by environmental factors in addition to body temperature. Thus, caution is required in extrapolating from laboratory acclimation studies to acclimatization effects in field populations. The seasonal and tidal-height variations in the heat-shock response are discussed in the context of energy costs of protein turnover.</description><subject>Acclimatization</subject><subject>Cellular biology</subject><subject>Gels</subject><subject>Heat shock proteins</subject><subject>Heat shock response</subject><subject>High temperature</subject><subject>Inductive reasoning</subject><subject>Marine</subject><subject>Mollusks</subject><subject>Mussels</subject><subject>Mytilus californianus</subject><subject>Physiology</subject><subject>Protein isoforms</subject><subject>Protein synthesis</subject><subject>Proteins</subject><subject>Sea water</subject><issn>0006-3185</issn><issn>1939-8697</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>79B</sourceid><recordid>eNqNkttu1DAQhiMEotuCeAMUIQ7tRcDHxOauWi1spSKQWq4tx3F2vWTjYDtVlxfgtRmapRVIIC6s0Xi--T0znix7gtFrQlH1BnNGKsLuZTMsqSxEKav72QwhVBYUC36QHca4ARcRzB5mB0RwgUWFZ9n3pdWpuFh78yX_FHyyrs8X10OwMTrf5-B92CXXjTE3unOtD73T_Rjf5qfGdG6rk_sGB8jjC6uj73WX677JL12ju2Jp3Wqd8rnfDjo4iMaTm-htLuQt2taaFB9lD1rdRft4b4-yz-8Wl_Nlcf7x_dn89LwwXPJUlFJgRiUqeSU0AVuXhuGypAhhTJnViJGatYi1TNekrlgrWslrTJDQVJaIHmVnk27curSGkqAdVTvfuCsboks7dUWU1-63u87VQYed8mGloJOkKOhJ0DqetIbgv442JrV10diu0731Y1QY6mOl4JQD-vLfKJeCUskAfPYHuPFjgLFGRQiSvEIlAejVBJngYwy2VUOAeUKFGKmf-6D2-wDk073cWG9tc8v9WgAAXkzAaNbO6JW_-fq7R--ETv6HU0PTAvt8Yjcx-fDX2n4AP4jX7w</recordid><startdate>19970401</startdate><enddate>19970401</enddate><creator>Roberts, Deirdre A.</creator><creator>Hofmann, Gretchen E.</creator><creator>Somero, George N.</creator><general>Marine Biological Laboratory</general><general>University of Chicago Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TN</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H95</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>79B</scope></search><sort><creationdate>19970401</creationdate><title>Heat-Shock Protein Expression in Mytilus californianus: Acclimatization (Seasonal and Tidal-Height Comparisons) and Acclimation Effects</title><author>Roberts, Deirdre A. ; 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Endogenous levels of heat-shock proteins in the 70-kDa class (hsp70 isoforms) and profiles of induction temperature for newly synthesized hsp70 were measured in freshly field-collected specimens as functions of location height in the intertidal and season, and in mussels after 7 weeks of laboratory thermal acclimation. There were significant differences in endogenous levels of hsp70 as functions of season and collection height. Strong induction of new hsp70 synthesis occurred at body temperatures within the range measured in field specimens. Profiles of hsp70 thermal induction varied significantly with season, but not with height of collection. In contrast to the large differences in hsp70 expression between winter- and summer-acclimatized mussels, no differences related to temperature occurred in the differently acclimated mussels. The differences found between the effects of field acclimatization and laboratory thermal acclimation suggest that the stress response is modulated by environmental factors in addition to body temperature. Thus, caution is required in extrapolating from laboratory acclimation studies to acclimatization effects in field populations. The seasonal and tidal-height variations in the heat-shock response are discussed in the context of energy costs of protein turnover.</abstract><cop>United States</cop><pub>Marine Biological Laboratory</pub><pmid>28581871</pmid><doi>10.2307/1542724</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Acclimatization Cellular biology Gels Heat shock proteins Heat shock response High temperature Inductive reasoning Marine Mollusks Mussels Mytilus californianus Physiology Protein isoforms Protein synthesis Proteins Sea water |
title | Heat-Shock Protein Expression in Mytilus californianus: Acclimatization (Seasonal and Tidal-Height Comparisons) and Acclimation Effects |
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