Mitochondrial DNA copy number is proportional to total cell DNA under a variety of growth conditions
Analyses of populations of NIH/3T3 cells for mitochondrial DNA under various conditions were made by use of an improved procedure. Cells were examined at different cell densities, at different stages of cell growth, and after the cells had been incubated with a carcinogen reported to affect mitochon...
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| Veröffentlicht in: | The Journal of biological chemistry 1990-09, Vol.265 (25), p.14802-14807 |
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| container_end_page | 14807 |
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| container_issue | 25 |
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| container_title | The Journal of biological chemistry |
| container_volume | 265 |
| creator | Shay, J W Pierce, D J Werbin, H |
| description | Analyses of populations of NIH/3T3 cells for mitochondrial DNA under various conditions were made by use of an improved procedure.
Cells were examined at different cell densities, at different stages of cell growth, and after the cells had been incubated
with a carcinogen reported to affect mitochondrial DNA replication, benzo[a]pyrene-epoxide. In addition, the analysis was
performed on three tumorigenic NIH/3T3 cell lines and one mouse bladder epithelial cell line and its tumorigenic derivative.
Small variations of mitochondrial DNA under these various conditions were detected easily and revealed a linear relationship
between mitochondrial DNA and total cell DNA. |
| doi_str_mv | 10.1016/S0021-9258(18)77184-6 |
| format | Article |
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Cells were examined at different cell densities, at different stages of cell growth, and after the cells had been incubated
with a carcinogen reported to affect mitochondrial DNA replication, benzo[a]pyrene-epoxide. In addition, the analysis was
performed on three tumorigenic NIH/3T3 cell lines and one mouse bladder epithelial cell line and its tumorigenic derivative.
Small variations of mitochondrial DNA under these various conditions were detected easily and revealed a linear relationship
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Cells were examined at different cell densities, at different stages of cell growth, and after the cells had been incubated
with a carcinogen reported to affect mitochondrial DNA replication, benzo[a]pyrene-epoxide. In addition, the analysis was
performed on three tumorigenic NIH/3T3 cell lines and one mouse bladder epithelial cell line and its tumorigenic derivative.
Small variations of mitochondrial DNA under these various conditions were detected easily and revealed a linear relationship
between mitochondrial DNA and total cell DNA.</description><subject>Animals</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>Clone Cells</subject><subject>DNA Probes</subject><subject>DNA Replication</subject><subject>DNA, Mitochondrial - genetics</subject><subject>DNA, Mitochondrial - isolation & purification</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Nucleic Acid Hybridization</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kF1PwyAYhYnRzDn9CUt6YYxeVHmhULg0fidTL9TEO8IK3TBrmdC67N_L3CIhgeSc83J4EBoDvgQM_OoNYwK5JEycg7goSxBFzvfQELCgOWXwuY-G_5ZDdBTjF06rkDBAA0JlwaUYIvPsOl_NfWuC04vs9uU6q_xynbV9M7UhczFbBr_0oXO-TXrn0-7SpbKLrbtvTfLp7EcHZ7t15utsFvyqm6c5rXGbXDxGB7VeRHuyO0fo4_7u_eYxn7w-PN1cT_KqAOhyMNyUvCioEASYsZpSo2ssObapNi6nrLR1-gE1hLKSSCsFx8bWBauIpVrSETrbzk2dv3sbO9W4uGmqW-v7qIBJAYlUMrKtsQo-xmBrtQyu0WGtAKsNXfVHV23QKRDqj67iKTfePdBPG2v-UzucST_d6nM3m69csGrqEl3bKMKZIkxBITChv_KmgRw</recordid><startdate>19900905</startdate><enddate>19900905</enddate><creator>Shay, J W</creator><creator>Pierce, D J</creator><creator>Werbin, H</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19900905</creationdate><title>Mitochondrial DNA copy number is proportional to total cell DNA under a variety of growth conditions</title><author>Shay, J W ; Pierce, D J ; Werbin, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-1d6d7644388215dea33daf0960e04907b57ef0043d235729e9860def45c2e3a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Cell Division</topic><topic>Cell Line</topic><topic>Clone Cells</topic><topic>DNA Probes</topic><topic>DNA Replication</topic><topic>DNA, Mitochondrial - genetics</topic><topic>DNA, Mitochondrial - isolation & purification</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Nucleic Acid Hybridization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shay, J W</creatorcontrib><creatorcontrib>Pierce, D J</creatorcontrib><creatorcontrib>Werbin, H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shay, J W</au><au>Pierce, D J</au><au>Werbin, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mitochondrial DNA copy number is proportional to total cell DNA under a variety of growth conditions</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1990-09-05</date><risdate>1990</risdate><volume>265</volume><issue>25</issue><spage>14802</spage><epage>14807</epage><pages>14802-14807</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Analyses of populations of NIH/3T3 cells for mitochondrial DNA under various conditions were made by use of an improved procedure.
Cells were examined at different cell densities, at different stages of cell growth, and after the cells had been incubated
with a carcinogen reported to affect mitochondrial DNA replication, benzo[a]pyrene-epoxide. In addition, the analysis was
performed on three tumorigenic NIH/3T3 cell lines and one mouse bladder epithelial cell line and its tumorigenic derivative.
Small variations of mitochondrial DNA under these various conditions were detected easily and revealed a linear relationship
between mitochondrial DNA and total cell DNA.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>2394698</pmid><doi>10.1016/S0021-9258(18)77184-6</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1990-09, Vol.265 (25), p.14802-14807 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15981771 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Cell Division Cell Line Clone Cells DNA Probes DNA Replication DNA, Mitochondrial - genetics DNA, Mitochondrial - isolation & purification Mice Mice, Inbred Strains Nucleic Acid Hybridization |
| title | Mitochondrial DNA copy number is proportional to total cell DNA under a variety of growth conditions |
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