Polyvinyl alcohol-glutaraldehyde network as a support for protein immobilisation
Polyvinyl alcohol-glutaraldehyde (PVA-glut) network was synthesised in bead and disc forms and used for protein immobilisation. Xanthine oxidase, alpha -amylase and amyloglucosidase were covalently fixed on the beads yielding preparations with specific activities retention of 72.3%, 1.6% and 1.4%, r...
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| Veröffentlicht in: | Biotechnology techniques 1997-02, Vol.11 (2), p.67-70 |
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| container_title | Biotechnology techniques |
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| creator | ARAUJO, A. M NEVES, M. T AZEVEDO, W. M OLIVEIRA, G. G FERREIRA, D. L COELHO, R. A. L FIGUEIREDO, E. A. P CARVALHO, L. B |
| description | Polyvinyl alcohol-glutaraldehyde (PVA-glut) network was synthesised in bead and disc forms and used for protein immobilisation. Xanthine oxidase, alpha -amylase and amyloglucosidase were covalently fixed on the beads yielding preparations with specific activities retention of 72.3%, 1.6% and 1.4%, respectively. Km of xanthine oxidase PVA-glut beads (24 plus or minus 4 mu M) was slightly higher than that estimated for the soluble enzyme (16 plus or minus 2 mu M). Antigens from Schistosoma mansoni were covalently fixed onto PVA-glut discs and ELISA was carried out. The relationship between the amount of fixed antigen and the results obtained by ELISA showed a hyperbolic curve and the saturation of antigen-antibody complex was achieved at the plateau. |
| doi_str_mv | 10.1023/A:1018404103626 |
| format | Article |
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M ; NEVES, M. T ; AZEVEDO, W. M ; OLIVEIRA, G. G ; FERREIRA, D. L ; COELHO, R. A. L ; FIGUEIREDO, E. A. P ; CARVALHO, L. B</creator><creatorcontrib>ARAUJO, A. M ; NEVES, M. T ; AZEVEDO, W. M ; OLIVEIRA, G. G ; FERREIRA, D. L ; COELHO, R. A. L ; FIGUEIREDO, E. A. P ; CARVALHO, L. B</creatorcontrib><description>Polyvinyl alcohol-glutaraldehyde (PVA-glut) network was synthesised in bead and disc forms and used for protein immobilisation. Xanthine oxidase, alpha -amylase and amyloglucosidase were covalently fixed on the beads yielding preparations with specific activities retention of 72.3%, 1.6% and 1.4%, respectively. Km of xanthine oxidase PVA-glut beads (24 plus or minus 4 mu M) was slightly higher than that estimated for the soluble enzyme (16 plus or minus 2 mu M). Antigens from Schistosoma mansoni were covalently fixed onto PVA-glut discs and ELISA was carried out. 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The relationship between the amount of fixed antigen and the results obtained by ELISA showed a hyperbolic curve and the saturation of antigen-antibody complex was achieved at the plateau.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immobilization of enzymes and other molecules</subject><subject>Immobilization techniques</subject><subject>Methods. Procedures. 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Technologies</topic><topic>Schistosoma mansoni</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ARAUJO, A. M</creatorcontrib><creatorcontrib>NEVES, M. T</creatorcontrib><creatorcontrib>AZEVEDO, W. M</creatorcontrib><creatorcontrib>OLIVEIRA, G. G</creatorcontrib><creatorcontrib>FERREIRA, D. L</creatorcontrib><creatorcontrib>COELHO, R. A. L</creatorcontrib><creatorcontrib>FIGUEIREDO, E. A. P</creatorcontrib><creatorcontrib>CARVALHO, L. B</creatorcontrib><collection>Pascal-Francis</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biotechnology techniques</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ARAUJO, A. M</au><au>NEVES, M. T</au><au>AZEVEDO, W. 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| ispartof | Biotechnology techniques, 1997-02, Vol.11 (2), p.67-70 |
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| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Immobilization of enzymes and other molecules Immobilization techniques Methods. Procedures. Technologies Schistosoma mansoni |
| title | Polyvinyl alcohol-glutaraldehyde network as a support for protein immobilisation |
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