Plasmid transformation of Clostridium perfringens by electroporation methods

Two electroporation methods were compared and modified to improve the frequencies of transfer of plasmid DNA into Clostridium perfringens. A plasmid shuttle vector, pSB92A2, containing chloramphenicol and ampicillin resistance genes and a clostridial origin of replication isolated from a cryptic C....

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Veröffentlicht in:	FEMS microbiology letters 1990, Vol.66 (1/3), p.221-226
1. Verfasser:	Phillips-Jones, M.K
Format:	Artikel
Sprache:	eng
Schlagworte:	chloramphenicol cloning cloning vector Clostridium perfringens electroporation Plasmid shuttle vector plasmids Transformation vectors
Online-Zugang:	Volltext
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