Passive transfer of antibody to Ehrlichia risticii protects mice from ehrlichiosis
Mice that recovered from Ehrlichia risticii infection were immune to a challenge dose of 100 50% lethal doses. Immune or normal mouse serum was passively transferred to mice challenged with E. risticii. Clinical signs of ehrlichiosis were completely prevented in 22 of 24 recipients of immune serum,...
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| Veröffentlicht in: | Infection and Immunity 1991-06, Vol.59 (6), p.2058-2062 |
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| creator | Kaylor, P.S. (Washington State University, Pullman, WA) Crawford, T.B McElwain, T.F Palmer, G.H |
| description | Mice that recovered from Ehrlichia risticii infection were immune to a challenge dose of 100 50% lethal doses. Immune or normal mouse serum was passively transferred to mice challenged with E. risticii. Clinical signs of ehrlichiosis were completely prevented in 22 of 24 recipients of immune serum, and the onset of signs of illness was delayed in the remaining two mice compared with the onset of illness in 24 of 24 recipients of nonimmune serum. Purified immunoglobulin G (IgG) was used to passively protect mice from infection with E. risticii. All 15 mice that received IgG from normal serum but none of the 15 mice that received IgG from immune serum developed clinical signs of illness. Antibodies in immune mouse serum immunoprecipitated [35S]methionine metabolically labeled E. risticii proteins with apparent molecular masses ranging from 14 to 90 kDa. The major antigens recognized by dilute immune serum in immunoblot analysis had molecular masses of 62, 53, 40, 33, 27, and 25 kDa, and the 62- and 27-kDa antigens were prominent in immunoprecipitations with dilute antibody. Antigens with molecular masses of 62, 53, 40, 33, and 27 kDa are likely surface exposed, as determined by immunoprecipitation of 125I-labeled organisms with immune mouse serum |
| doi_str_mv | 10.1128/IAI.59.6.2058-2062.1991 |
| format | Article |
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(Washington State University, Pullman, WA) ; Crawford, T.B ; McElwain, T.F ; Palmer, G.H</creator><creatorcontrib>Kaylor, P.S. (Washington State University, Pullman, WA) ; Crawford, T.B ; McElwain, T.F ; Palmer, G.H</creatorcontrib><description>Mice that recovered from Ehrlichia risticii infection were immune to a challenge dose of 100 50% lethal doses. Immune or normal mouse serum was passively transferred to mice challenged with E. risticii. Clinical signs of ehrlichiosis were completely prevented in 22 of 24 recipients of immune serum, and the onset of signs of illness was delayed in the remaining two mice compared with the onset of illness in 24 of 24 recipients of nonimmune serum. Purified immunoglobulin G (IgG) was used to passively protect mice from infection with E. risticii. All 15 mice that received IgG from normal serum but none of the 15 mice that received IgG from immune serum developed clinical signs of illness. Antibodies in immune mouse serum immunoprecipitated [35S]methionine metabolically labeled E. risticii proteins with apparent molecular masses ranging from 14 to 90 kDa. The major antigens recognized by dilute immune serum in immunoblot analysis had molecular masses of 62, 53, 40, 33, 27, and 25 kDa, and the 62- and 27-kDa antigens were prominent in immunoprecipitations with dilute antibody. Antigens with molecular masses of 62, 53, 40, 33, and 27 kDa are likely surface exposed, as determined by immunoprecipitation of 125I-labeled organisms with immune mouse serum</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/IAI.59.6.2058-2062.1991</identifier><identifier>PMID: 2037365</identifier><identifier>CODEN: INFIBR</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Animals ; Antibodies, Bacterial - administration & dosage ; Antibody production ; ANTIGENE ; ANTIGENOS ; Antigens, Bacterial - immunology ; Biological and medical sciences ; Disease Models, Animal ; EHRLICHIA ; Ehrlichia - immunology ; Electrophoresis, Polyacrylamide Gel ; EXPERIMENTACION IN VIVO ; EXPERIMENTATION IN VIVO ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; IMMUNITE ; Immunization, Passive ; Immunobiology ; Immunoblotting ; Immunoglobulin G - immunology ; IMMUNSERUM ; INFECCION ; INFECTION ; INMUNIDAD ; Lethal Dose 50 ; Mice ; Mice, Inbred Strains ; MODELE ; MODELOS ; Molecular Weight ; Precipitin Tests ; RATON ; Rickettsiaceae Infections - immunology ; Rickettsiaceae Infections - prevention & control ; SOURIS ; SUERO INMUNE</subject><ispartof>Infection and Immunity, 1991-06, Vol.59 (6), p.2058-2062</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c571t-3f4caa35d1c4e7696ea3d4342eb64fba97ff704c7ca4a5d7ffaa4ccd329814fc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC257965/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC257965/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19857300$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2037365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaylor, P.S. (Washington State University, Pullman, WA)</creatorcontrib><creatorcontrib>Crawford, T.B</creatorcontrib><creatorcontrib>McElwain, T.F</creatorcontrib><creatorcontrib>Palmer, G.H</creatorcontrib><title>Passive transfer of antibody to Ehrlichia risticii protects mice from ehrlichiosis</title><title>Infection and Immunity</title><addtitle>Infect Immun</addtitle><description>Mice that recovered from Ehrlichia risticii infection were immune to a challenge dose of 100 50% lethal doses. Immune or normal mouse serum was passively transferred to mice challenged with E. risticii. Clinical signs of ehrlichiosis were completely prevented in 22 of 24 recipients of immune serum, and the onset of signs of illness was delayed in the remaining two mice compared with the onset of illness in 24 of 24 recipients of nonimmune serum. Purified immunoglobulin G (IgG) was used to passively protect mice from infection with E. risticii. All 15 mice that received IgG from normal serum but none of the 15 mice that received IgG from immune serum developed clinical signs of illness. Antibodies in immune mouse serum immunoprecipitated [35S]methionine metabolically labeled E. risticii proteins with apparent molecular masses ranging from 14 to 90 kDa. The major antigens recognized by dilute immune serum in immunoblot analysis had molecular masses of 62, 53, 40, 33, 27, and 25 kDa, and the 62- and 27-kDa antigens were prominent in immunoprecipitations with dilute antibody. Antigens with molecular masses of 62, 53, 40, 33, and 27 kDa are likely surface exposed, as determined by immunoprecipitation of 125I-labeled organisms with immune mouse serum</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Antibodies, Bacterial - administration & dosage</subject><subject>Antibody production</subject><subject>ANTIGENE</subject><subject>ANTIGENOS</subject><subject>Antigens, Bacterial - immunology</subject><subject>Biological and medical sciences</subject><subject>Disease Models, Animal</subject><subject>EHRLICHIA</subject><subject>Ehrlichia - immunology</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>EXPERIMENTACION IN VIVO</subject><subject>EXPERIMENTATION IN VIVO</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>IMMUNITE</subject><subject>Immunization, Passive</subject><subject>Immunobiology</subject><subject>Immunoblotting</subject><subject>Immunoglobulin G - immunology</subject><subject>IMMUNSERUM</subject><subject>INFECCION</subject><subject>INFECTION</subject><subject>INMUNIDAD</subject><subject>Lethal Dose 50</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>MODELE</subject><subject>MODELOS</subject><subject>Molecular Weight</subject><subject>Precipitin Tests</subject><subject>RATON</subject><subject>Rickettsiaceae Infections - immunology</subject><subject>Rickettsiaceae Infections - prevention & control</subject><subject>SOURIS</subject><subject>SUERO INMUNE</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1u1DAUhS0EKkPhBZAQZgG7BP_G8aKLqip0pEqtgK6tO449MUriwc4U9e3xaKKWrqyr8517bB-EPlJSU8rar-vzdS113dSMyLZipGE11Zq-QCtKdFtJydhLtCKE6krLRr1Gb3L-XUYhRHuCThjhijdyhX7cQs7h3uE5wZS9Szh6DNMcNrF7wHPEl30agu0D4BTyHGwIeJfi7Oyc8Riswz7FEbuFijnkt-iVhyG7d8t5iu6-Xf66uKqub76vL86vKysVnSvuhQXgsqNWONXoxgHvBBfMbRrhN6CV94oIqywIkF2ZAIS1HWe6pcJbforOjnt3-83oOuum8obB7FIYIT2YCME8V6bQm228N0wq3cji_7L4U_yzd3k2Y8jWDQNMLu6zoVIrrjQvoDqCNsWck_OPGZSYQxsmlCypTWMObZhDG-bQRnF--P-Kj77l-4v-edEhWxh86cCG_LRet1JxQgr36cj1Ydv_DckZyOPz1MK8PzIeooFtacvc_dScCUUk_we3hKo5</recordid><startdate>19910601</startdate><enddate>19910601</enddate><creator>Kaylor, P.S. (Washington State University, Pullman, WA)</creator><creator>Crawford, T.B</creator><creator>McElwain, T.F</creator><creator>Palmer, G.H</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>19910601</creationdate><title>Passive transfer of antibody to Ehrlichia risticii protects mice from ehrlichiosis</title><author>Kaylor, P.S. (Washington State University, Pullman, WA) ; Crawford, T.B ; McElwain, T.F ; Palmer, G.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c571t-3f4caa35d1c4e7696ea3d4342eb64fba97ff704c7ca4a5d7ffaa4ccd329814fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Antibodies, Bacterial - administration & dosage</topic><topic>Antibody production</topic><topic>ANTIGENE</topic><topic>ANTIGENOS</topic><topic>Antigens, Bacterial - immunology</topic><topic>Biological and medical sciences</topic><topic>Disease Models, Animal</topic><topic>EHRLICHIA</topic><topic>Ehrlichia - immunology</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>EXPERIMENTACION IN VIVO</topic><topic>EXPERIMENTATION IN VIVO</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>IMMUNITE</topic><topic>Immunization, Passive</topic><topic>Immunobiology</topic><topic>Immunoblotting</topic><topic>Immunoglobulin G - immunology</topic><topic>IMMUNSERUM</topic><topic>INFECCION</topic><topic>INFECTION</topic><topic>INMUNIDAD</topic><topic>Lethal Dose 50</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>MODELE</topic><topic>MODELOS</topic><topic>Molecular Weight</topic><topic>Precipitin Tests</topic><topic>RATON</topic><topic>Rickettsiaceae Infections - immunology</topic><topic>Rickettsiaceae Infections - prevention & control</topic><topic>SOURIS</topic><topic>SUERO INMUNE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaylor, P.S. (Washington State University, Pullman, WA)</creatorcontrib><creatorcontrib>Crawford, T.B</creatorcontrib><creatorcontrib>McElwain, T.F</creatorcontrib><creatorcontrib>Palmer, G.H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Infection and Immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaylor, P.S. (Washington State University, Pullman, WA)</au><au>Crawford, T.B</au><au>McElwain, T.F</au><au>Palmer, G.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Passive transfer of antibody to Ehrlichia risticii protects mice from ehrlichiosis</atitle><jtitle>Infection and Immunity</jtitle><addtitle>Infect Immun</addtitle><date>1991-06-01</date><risdate>1991</risdate><volume>59</volume><issue>6</issue><spage>2058</spage><epage>2062</epage><pages>2058-2062</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><coden>INFIBR</coden><abstract>Mice that recovered from Ehrlichia risticii infection were immune to a challenge dose of 100 50% lethal doses. Immune or normal mouse serum was passively transferred to mice challenged with E. risticii. Clinical signs of ehrlichiosis were completely prevented in 22 of 24 recipients of immune serum, and the onset of signs of illness was delayed in the remaining two mice compared with the onset of illness in 24 of 24 recipients of nonimmune serum. Purified immunoglobulin G (IgG) was used to passively protect mice from infection with E. risticii. All 15 mice that received IgG from normal serum but none of the 15 mice that received IgG from immune serum developed clinical signs of illness. Antibodies in immune mouse serum immunoprecipitated [35S]methionine metabolically labeled E. risticii proteins with apparent molecular masses ranging from 14 to 90 kDa. The major antigens recognized by dilute immune serum in immunoblot analysis had molecular masses of 62, 53, 40, 33, 27, and 25 kDa, and the 62- and 27-kDa antigens were prominent in immunoprecipitations with dilute antibody. Antigens with molecular masses of 62, 53, 40, 33, and 27 kDa are likely surface exposed, as determined by immunoprecipitation of 125I-labeled organisms with immune mouse serum</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>2037365</pmid><doi>10.1128/IAI.59.6.2058-2062.1991</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Infection and Immunity, 1991-06, Vol.59 (6), p.2058-2062 |
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| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Analysis of the immune response. Humoral and cellular immunity Animals Antibodies, Bacterial - administration & dosage Antibody production ANTIGENE ANTIGENOS Antigens, Bacterial - immunology Biological and medical sciences Disease Models, Animal EHRLICHIA Ehrlichia - immunology Electrophoresis, Polyacrylamide Gel EXPERIMENTACION IN VIVO EXPERIMENTATION IN VIVO Female Fundamental and applied biological sciences. Psychology Fundamental immunology IMMUNITE Immunization, Passive Immunobiology Immunoblotting Immunoglobulin G - immunology IMMUNSERUM INFECCION INFECTION INMUNIDAD Lethal Dose 50 Mice Mice, Inbred Strains MODELE MODELOS Molecular Weight Precipitin Tests RATON Rickettsiaceae Infections - immunology Rickettsiaceae Infections - prevention & control SOURIS SUERO INMUNE |
| title | Passive transfer of antibody to Ehrlichia risticii protects mice from ehrlichiosis |
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