Transient transformation of maize tissues by microparticle bombardment

Transient transformation of maize coleoptiles with the β-glucuronidase gene was achieved with a particle gun built by us essentially according to the concept of Klein et al. (Nature, 327 (1987) 70–73). Either tungsten or gold particles were used to transform coleoptiles. Transformed blue cells were...

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Veröffentlicht in:	Plant science (Limerick) 1991, Vol.75 (2), p.237-243
Hauptverfasser:	Reggiardo, Martin I., Arana, Jose Luis, Orsaria, Lelia M., Permingeat, Hugo R., Spitteler, Marcelo A., Vallejos, Ruben H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology gene transfer Genetic engineering applications Genetics and breeding of economic plants maize cell transformation particle gun Plant breeding: fundamental aspects and methodology Zea mays β-glucuronidase
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