Transient transformation of maize tissues by microparticle bombardment

Transient transformation of maize coleoptiles with the β-glucuronidase gene was achieved with a particle gun built by us essentially according to the concept of Klein et al. (Nature, 327 (1987) 70–73). Either tungsten or gold particles were used to transform coleoptiles. Transformed blue cells were...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Plant science (Limerick) 1991, Vol.75 (2), p.237-243
Hauptverfasser: Reggiardo, Martin I., Arana, Jose Luis, Orsaria, Lelia M., Permingeat, Hugo R., Spitteler, Marcelo A., Vallejos, Ruben H.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 243
container_issue 2
container_start_page 237
container_title Plant science (Limerick)
container_volume 75
creator Reggiardo, Martin I.
Arana, Jose Luis
Orsaria, Lelia M.
Permingeat, Hugo R.
Spitteler, Marcelo A.
Vallejos, Ruben H.
description Transient transformation of maize coleoptiles with the β-glucuronidase gene was achieved with a particle gun built by us essentially according to the concept of Klein et al. (Nature, 327 (1987) 70–73). Either tungsten or gold particles were used to transform coleoptiles. Transformed blue cells were found in different cell layers and occasionally in as deep as the 8th layer. A transformation efficiency of up to 2% was obtained when the gene was in plasmid pAI 1GUS driven by the alcohol dehydrogenase promoter plus the intron 1. The 35S promoter of cauliflower mosaic virus (plasmid pBI221) was ten times less efficient. Other maize tissues like immature embryos, leaf basal segments and cell suspensions were also transformed but at lower rates. The time course of the histochemical reaction for the β-glucuronidase activity showed that the number of transformed blue cells detected increased up to 72 h of starting the reaction. It is concluded that transformation of maize coleoptiles with the particle gun is a good method for testing gene expression in transient systems.
doi_str_mv 10.1016/0168-9452(91)90239-5
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_15973745</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0168945291902395</els_id><sourcerecordid>15973745</sourcerecordid><originalsourceid>FETCH-LOGICAL-c364t-26935c13e4530dd8ccef23274f1443c399aa56d5889f6c2f2ace3c120967e3603</originalsourceid><addsrcrecordid>eNp9kE9LAzEQxYMoWKvfwMMeRPSwmj-b3c1FkGJVKHip55DOTiCy29QkFfTTm7WlRw_DzOG9NzM_Qi4ZvWOU1fe52lJVkt8odqsoF6qUR2TC2kaUnEt1TCYHySk5i_GDUsqlbCZkvgxmHR2uU5HGyfowmOT8uvC2GIz7wSK5GLcYi9V3MTgIfmNCctBjsfLDyoRuyOZzcmJNH_Fi36fkff60nL2Ui7fn19njogRRV6nktRISmMBKCtp1LQBaLnhTWVZVAoRSxsi6k22rbA3ccgMogHGq6gZFTcWUXO9yN8F_5qOSHlwE7HuzRr-NmknViCanT0m1E-aDYwxo9Sa4wYRvzageoemRiB6JaMX0HzQ92q72-SaC6W1GAi4evJLSVnCWZQ87GeZfvxwGHSEzBOxcQEi68-7_Pb9NuIBm</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15973745</pqid></control><display><type>article</type><title>Transient transformation of maize tissues by microparticle bombardment</title><source>Elsevier ScienceDirect Journals Complete</source><creator>Reggiardo, Martin I. ; Arana, Jose Luis ; Orsaria, Lelia M. ; Permingeat, Hugo R. ; Spitteler, Marcelo A. ; Vallejos, Ruben H.</creator><creatorcontrib>Reggiardo, Martin I. ; Arana, Jose Luis ; Orsaria, Lelia M. ; Permingeat, Hugo R. ; Spitteler, Marcelo A. ; Vallejos, Ruben H.</creatorcontrib><description>Transient transformation of maize coleoptiles with the β-glucuronidase gene was achieved with a particle gun built by us essentially according to the concept of Klein et al. (Nature, 327 (1987) 70–73). Either tungsten or gold particles were used to transform coleoptiles. Transformed blue cells were found in different cell layers and occasionally in as deep as the 8th layer. A transformation efficiency of up to 2% was obtained when the gene was in plasmid pAI 1GUS driven by the alcohol dehydrogenase promoter plus the intron 1. The 35S promoter of cauliflower mosaic virus (plasmid pBI221) was ten times less efficient. Other maize tissues like immature embryos, leaf basal segments and cell suspensions were also transformed but at lower rates. The time course of the histochemical reaction for the β-glucuronidase activity showed that the number of transformed blue cells detected increased up to 72 h of starting the reaction. It is concluded that transformation of maize coleoptiles with the particle gun is a good method for testing gene expression in transient systems.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/0168-9452(91)90239-5</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; gene transfer ; Genetic engineering applications ; Genetics and breeding of economic plants ; maize cell transformation ; particle gun ; Plant breeding: fundamental aspects and methodology ; Zea mays ; β-glucuronidase</subject><ispartof>Plant science (Limerick), 1991, Vol.75 (2), p.237-243</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c364t-26935c13e4530dd8ccef23274f1443c399aa56d5889f6c2f2ace3c120967e3603</citedby><cites>FETCH-LOGICAL-c364t-26935c13e4530dd8ccef23274f1443c399aa56d5889f6c2f2ace3c120967e3603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0168-9452(91)90239-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=5008321$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Reggiardo, Martin I.</creatorcontrib><creatorcontrib>Arana, Jose Luis</creatorcontrib><creatorcontrib>Orsaria, Lelia M.</creatorcontrib><creatorcontrib>Permingeat, Hugo R.</creatorcontrib><creatorcontrib>Spitteler, Marcelo A.</creatorcontrib><creatorcontrib>Vallejos, Ruben H.</creatorcontrib><title>Transient transformation of maize tissues by microparticle bombardment</title><title>Plant science (Limerick)</title><description>Transient transformation of maize coleoptiles with the β-glucuronidase gene was achieved with a particle gun built by us essentially according to the concept of Klein et al. (Nature, 327 (1987) 70–73). Either tungsten or gold particles were used to transform coleoptiles. Transformed blue cells were found in different cell layers and occasionally in as deep as the 8th layer. A transformation efficiency of up to 2% was obtained when the gene was in plasmid pAI 1GUS driven by the alcohol dehydrogenase promoter plus the intron 1. The 35S promoter of cauliflower mosaic virus (plasmid pBI221) was ten times less efficient. Other maize tissues like immature embryos, leaf basal segments and cell suspensions were also transformed but at lower rates. The time course of the histochemical reaction for the β-glucuronidase activity showed that the number of transformed blue cells detected increased up to 72 h of starting the reaction. It is concluded that transformation of maize coleoptiles with the particle gun is a good method for testing gene expression in transient systems.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene transfer</subject><subject>Genetic engineering applications</subject><subject>Genetics and breeding of economic plants</subject><subject>maize cell transformation</subject><subject>particle gun</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Zea mays</subject><subject>β-glucuronidase</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LAzEQxYMoWKvfwMMeRPSwmj-b3c1FkGJVKHip55DOTiCy29QkFfTTm7WlRw_DzOG9NzM_Qi4ZvWOU1fe52lJVkt8odqsoF6qUR2TC2kaUnEt1TCYHySk5i_GDUsqlbCZkvgxmHR2uU5HGyfowmOT8uvC2GIz7wSK5GLcYi9V3MTgIfmNCctBjsfLDyoRuyOZzcmJNH_Fi36fkff60nL2Ui7fn19njogRRV6nktRISmMBKCtp1LQBaLnhTWVZVAoRSxsi6k22rbA3ccgMogHGq6gZFTcWUXO9yN8F_5qOSHlwE7HuzRr-NmknViCanT0m1E-aDYwxo9Sa4wYRvzageoemRiB6JaMX0HzQ92q72-SaC6W1GAi4evJLSVnCWZQ87GeZfvxwGHSEzBOxcQEi68-7_Pb9NuIBm</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>Reggiardo, Martin I.</creator><creator>Arana, Jose Luis</creator><creator>Orsaria, Lelia M.</creator><creator>Permingeat, Hugo R.</creator><creator>Spitteler, Marcelo A.</creator><creator>Vallejos, Ruben H.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>1991</creationdate><title>Transient transformation of maize tissues by microparticle bombardment</title><author>Reggiardo, Martin I. ; Arana, Jose Luis ; Orsaria, Lelia M. ; Permingeat, Hugo R. ; Spitteler, Marcelo A. ; Vallejos, Ruben H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-26935c13e4530dd8ccef23274f1443c399aa56d5889f6c2f2ace3c120967e3603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene transfer</topic><topic>Genetic engineering applications</topic><topic>Genetics and breeding of economic plants</topic><topic>maize cell transformation</topic><topic>particle gun</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Zea mays</topic><topic>β-glucuronidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reggiardo, Martin I.</creatorcontrib><creatorcontrib>Arana, Jose Luis</creatorcontrib><creatorcontrib>Orsaria, Lelia M.</creatorcontrib><creatorcontrib>Permingeat, Hugo R.</creatorcontrib><creatorcontrib>Spitteler, Marcelo A.</creatorcontrib><creatorcontrib>Vallejos, Ruben H.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reggiardo, Martin I.</au><au>Arana, Jose Luis</au><au>Orsaria, Lelia M.</au><au>Permingeat, Hugo R.</au><au>Spitteler, Marcelo A.</au><au>Vallejos, Ruben H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transient transformation of maize tissues by microparticle bombardment</atitle><jtitle>Plant science (Limerick)</jtitle><date>1991</date><risdate>1991</risdate><volume>75</volume><issue>2</issue><spage>237</spage><epage>243</epage><pages>237-243</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>Transient transformation of maize coleoptiles with the β-glucuronidase gene was achieved with a particle gun built by us essentially according to the concept of Klein et al. (Nature, 327 (1987) 70–73). Either tungsten or gold particles were used to transform coleoptiles. Transformed blue cells were found in different cell layers and occasionally in as deep as the 8th layer. A transformation efficiency of up to 2% was obtained when the gene was in plasmid pAI 1GUS driven by the alcohol dehydrogenase promoter plus the intron 1. The 35S promoter of cauliflower mosaic virus (plasmid pBI221) was ten times less efficient. Other maize tissues like immature embryos, leaf basal segments and cell suspensions were also transformed but at lower rates. The time course of the histochemical reaction for the β-glucuronidase activity showed that the number of transformed blue cells detected increased up to 72 h of starting the reaction. It is concluded that transformation of maize coleoptiles with the particle gun is a good method for testing gene expression in transient systems.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><doi>10.1016/0168-9452(91)90239-5</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0168-9452
ispartof Plant science (Limerick), 1991, Vol.75 (2), p.237-243
issn 0168-9452
1873-2259
language eng
recordid cdi_proquest_miscellaneous_15973745
source Elsevier ScienceDirect Journals Complete
subjects Agronomy. Soil science and plant productions
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
gene transfer
Genetic engineering applications
Genetics and breeding of economic plants
maize cell transformation
particle gun
Plant breeding: fundamental aspects and methodology
Zea mays
β-glucuronidase
title Transient transformation of maize tissues by microparticle bombardment
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T15%3A06%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transient%20transformation%20of%20maize%20tissues%20by%20microparticle%20bombardment&rft.jtitle=Plant%20science%20(Limerick)&rft.au=Reggiardo,%20Martin%20I.&rft.date=1991&rft.volume=75&rft.issue=2&rft.spage=237&rft.epage=243&rft.pages=237-243&rft.issn=0168-9452&rft.eissn=1873-2259&rft.coden=PLSCE4&rft_id=info:doi/10.1016/0168-9452(91)90239-5&rft_dat=%3Cproquest_cross%3E15973745%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15973745&rft_id=info:pmid/&rft_els_id=0168945291902395&rfr_iscdi=true