A mutation that converts serine super(340) of the HsdS sub(K) polypeptide to phenylalanine and its effects on restriction and modification in Escherichia coli) K-12

A mutation that converts serine super(340) of the HsdS sub(K) polypeptide to phenylalanine and its effects on restriction and modification in Escherichia coli) K-12

A hybrid hsdS gene, encoding the HsdS sub(t) sub(s) super(+) super(d) polypeptide, was constructed by joining the proximal region of the wild-type hsdS sequence with the distal region of the hsdS sub(t) sub(s) super(+) super(d) sequence, at the hsdS BglII site. The hybrid hsdS-S sub(t) sub(s) super(...

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Veröffentlicht in:Gene 1990-01, Vol.90 (1), p.125-128
Hauptverfasser: Zinkevich, V E, Weiserova, M, Kryukov, V M, Hubacek, J
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container_title Gene
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creator Zinkevich, V E
Weiserova, M
Kryukov, V M
Hubacek, J
description A hybrid hsdS gene, encoding the HsdS sub(t) sub(s) super(+) super(d) polypeptide, was constructed by joining the proximal region of the wild-type hsdS sequence with the distal region of the hsdS sub(t) sub(s) super(+) super(d) sequence, at the hsdS BglII site. The hybrid hsdS-S sub(t) sub(s) super(+) super(d) gene exerts a trans-dominant effect on restriction and modification, which points to the location of the temperature-sensitive trans-dominant (+d) mutation in the gene hsdS sub(t) sub(s) super(+) super(d) distal region. Sequencing of the region downstream from the HindIII target in the Escherichia coli) K-12 hsdS sub(t) sub(s) super(+) super(d) mutant was carried out.
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title A mutation that converts serine super(340) of the HsdS sub(K) polypeptide to phenylalanine and its effects on restriction and modification in Escherichia coli) K-12
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