Neutral red treated plant cells show reduced affinity for nuclease/gold complexes
Fixation of bean root tips with glutaraldehyde containing the dye, neutral red, almost totally blocked the binding of RNase/gold to cytoplasmic ribosomes, RER and, within the nucleus, to the nucleolus and nuclear matrix. The binding of DNase/gold in plastids and the nucleus was only partially reduce...
Gespeichert in:
Veröffentlicht in: | Cell biology international reports 1990, Vol.14 (10), p.897-904 |
---|---|
1. Verfasser: | |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 904 |
---|---|
container_issue | 10 |
container_start_page | 897 |
container_title | Cell biology international reports |
container_volume | 14 |
creator | Lawton, June R |
description | Fixation of bean root tips with glutaraldehyde containing the dye, neutral red, almost totally blocked the binding of RNase/gold to cytoplasmic ribosomes, RER and, within the nucleus, to the nucleolus and nuclear matrix. The binding of DNase/gold in plastids and the nucleus was only partially reduced. It was concluded that neutral red intercalated into RNA molecules and possibly into some DNA molecules and inhibited enzymic binding. |
doi_str_mv | 10.1016/0309-1651(90)91159-2 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_15946870</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0309165190911592</els_id><sourcerecordid>15946870</sourcerecordid><originalsourceid>FETCH-LOGICAL-c314t-2efe9cd31be027ca8c07f189427d1290089ad5bf2b2d0ba912889e9e3c27db803</originalsourceid><addsrcrecordid>eNp9kEtLxDAQgIMouK7-Aw-9KHqom6Sv5CLI4gsWRVDwFtJ0opFsW5NU3X9v6i568zQM883rQ-iQ4DOCSTnDGeYpKQtywvEpJ6TgKd1CE8IqltIcP2-jyS-yi_a8f8Mxzwo-QQ93MAQnbeKgSYIDGWLsrWxDosBan_jX7nMsDioWpNamNWGV6M4l7aAsSA-zl842ieqWvYUv8PtoR0vr4WATp-jp6vJxfpMu7q9v5xeLVGUkDykFDVw1GakB00pJpnClCeM5rRpCOcaMy6aoNa1pg2vJCWWMA4dMRaBmOJui4_Xc3nXvA_gglsaPN8sWusGLaCEvWTWC-RpUrvPegRa9M0vpVoJgMfoToxwxyhEcix9_gsa2o8186ZW02slWGf_Xy8ui4KyI3Pmag_jshwEnvDLQRl3GgQqi6cz_i74BG1CErQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15946870</pqid></control><display><type>article</type><title>Neutral red treated plant cells show reduced affinity for nuclease/gold complexes</title><source>Alma/SFX Local Collection</source><creator>Lawton, June R</creator><creatorcontrib>Lawton, June R</creatorcontrib><description>Fixation of bean root tips with glutaraldehyde containing the dye, neutral red, almost totally blocked the binding of RNase/gold to cytoplasmic ribosomes, RER and, within the nucleus, to the nucleolus and nuclear matrix. The binding of DNase/gold in plastids and the nucleus was only partially reduced. It was concluded that neutral red intercalated into RNA molecules and possibly into some DNA molecules and inhibited enzymic binding.</description><identifier>ISSN: 0309-1651</identifier><identifier>EISSN: 1878-240X</identifier><identifier>DOI: 10.1016/0309-1651(90)91159-2</identifier><identifier>CODEN: CBRPDS</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Biological and medical sciences ; Diverse techniques ; Fundamental and applied biological sciences. Psychology ; Molecular and cellular biology ; Phaseolus vulgaris</subject><ispartof>Cell biology international reports, 1990, Vol.14 (10), p.897-904</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,4012,27912,27913,27914</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19655985$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Lawton, June R</creatorcontrib><title>Neutral red treated plant cells show reduced affinity for nuclease/gold complexes</title><title>Cell biology international reports</title><description>Fixation of bean root tips with glutaraldehyde containing the dye, neutral red, almost totally blocked the binding of RNase/gold to cytoplasmic ribosomes, RER and, within the nucleus, to the nucleolus and nuclear matrix. The binding of DNase/gold in plastids and the nucleus was only partially reduced. It was concluded that neutral red intercalated into RNA molecules and possibly into some DNA molecules and inhibited enzymic binding.</description><subject>Biological and medical sciences</subject><subject>Diverse techniques</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Molecular and cellular biology</subject><subject>Phaseolus vulgaris</subject><issn>0309-1651</issn><issn>1878-240X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLxDAQgIMouK7-Aw-9KHqom6Sv5CLI4gsWRVDwFtJ0opFsW5NU3X9v6i568zQM883rQ-iQ4DOCSTnDGeYpKQtywvEpJ6TgKd1CE8IqltIcP2-jyS-yi_a8f8Mxzwo-QQ93MAQnbeKgSYIDGWLsrWxDosBan_jX7nMsDioWpNamNWGV6M4l7aAsSA-zl842ieqWvYUv8PtoR0vr4WATp-jp6vJxfpMu7q9v5xeLVGUkDykFDVw1GakB00pJpnClCeM5rRpCOcaMy6aoNa1pg2vJCWWMA4dMRaBmOJui4_Xc3nXvA_gglsaPN8sWusGLaCEvWTWC-RpUrvPegRa9M0vpVoJgMfoToxwxyhEcix9_gsa2o8186ZW02slWGf_Xy8ui4KyI3Pmag_jshwEnvDLQRl3GgQqi6cz_i74BG1CErQ</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Lawton, June R</creator><general>Elsevier B.V</general><general>Academic Press</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>1990</creationdate><title>Neutral red treated plant cells show reduced affinity for nuclease/gold complexes</title><author>Lawton, June R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c314t-2efe9cd31be027ca8c07f189427d1290089ad5bf2b2d0ba912889e9e3c27db803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Biological and medical sciences</topic><topic>Diverse techniques</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Molecular and cellular biology</topic><topic>Phaseolus vulgaris</topic><toplevel>online_resources</toplevel><creatorcontrib>Lawton, June R</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Cell biology international reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lawton, June R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neutral red treated plant cells show reduced affinity for nuclease/gold complexes</atitle><jtitle>Cell biology international reports</jtitle><date>1990</date><risdate>1990</risdate><volume>14</volume><issue>10</issue><spage>897</spage><epage>904</epage><pages>897-904</pages><issn>0309-1651</issn><eissn>1878-240X</eissn><coden>CBRPDS</coden><abstract>Fixation of bean root tips with glutaraldehyde containing the dye, neutral red, almost totally blocked the binding of RNase/gold to cytoplasmic ribosomes, RER and, within the nucleus, to the nucleolus and nuclear matrix. The binding of DNase/gold in plastids and the nucleus was only partially reduced. It was concluded that neutral red intercalated into RNA molecules and possibly into some DNA molecules and inhibited enzymic binding.</abstract><cop>London</cop><cop>San Diego, CA</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><doi>10.1016/0309-1651(90)91159-2</doi><tpages>8</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0309-1651 |
ispartof | Cell biology international reports, 1990, Vol.14 (10), p.897-904 |
issn | 0309-1651 1878-240X |
language | eng |
recordid | cdi_proquest_miscellaneous_15946870 |
source | Alma/SFX Local Collection |
subjects | Biological and medical sciences Diverse techniques Fundamental and applied biological sciences. Psychology Molecular and cellular biology Phaseolus vulgaris |
title | Neutral red treated plant cells show reduced affinity for nuclease/gold complexes |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T09%3A10%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Neutral%20red%20treated%20plant%20cells%20show%20reduced%20affinity%20for%20nuclease/gold%20complexes&rft.jtitle=Cell%20biology%20international%20reports&rft.au=Lawton,%20June%20R&rft.date=1990&rft.volume=14&rft.issue=10&rft.spage=897&rft.epage=904&rft.pages=897-904&rft.issn=0309-1651&rft.eissn=1878-240X&rft.coden=CBRPDS&rft_id=info:doi/10.1016/0309-1651(90)91159-2&rft_dat=%3Cproquest_cross%3E15946870%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15946870&rft_id=info:pmid/&rft_els_id=0309165190911592&rfr_iscdi=true |