Photo-induced activation of cytochrome P450/reductase fusion enzyme coupled with spinach chloroplasts
Photoactivation of cytochrome P450 monooxygenase was studied using a combination of spinach chloroplasts and yeast microsomes containing rat P4501A1/yeast reductase fusion enzyme. Under illumination, in the reaction mixture, NADP was reduced, transferring electrons to the P450/reductase fusion enzym...
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| Veröffentlicht in: | Biotechnology techniques 1996-10, Vol.10 (10), p.717-720 |
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| container_end_page | 720 |
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| container_issue | 10 |
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| container_title | Biotechnology techniques |
| container_volume | 10 |
| creator | Kim, Y.S Hara, M Ikebukuro, K Miyake, J Ohkawa, H Karube, I |
| description | Photoactivation of cytochrome P450 monooxygenase was studied using a combination of spinach chloroplasts and yeast microsomes containing rat P4501A1/yeast reductase fusion enzyme. Under illumination, in the reaction mixture, NADP was reduced, transferring electrons to the P450/reductase fusion enzyme to convert 7-ethoxycoumarin to 7-hydroxycoumarin. |
| doi_str_mv | 10.1007/BF00222553 |
| format | Article |
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Under illumination, in the reaction mixture, NADP was reduced, transferring electrons to the P450/reductase fusion enzyme to convert 7-ethoxycoumarin to 7-hydroxycoumarin.</description><identifier>ISSN: 0951-208X</identifier><identifier>EISSN: 1573-6784</identifier><identifier>DOI: 10.1007/BF00222553</identifier><identifier>CODEN: BTECE6</identifier><language>eng</language><publisher>London: Chapman and Hall</publisher><subject>7-ethoxycoumarin ; 7-hydroxycoumarin ; Biological and medical sciences ; Biotechnology ; chemical reactions ; chloroplasts ; Clostridium pasteurianum ; coumarins ; cytochrome P-450 ; de-ethylation ; electron transfer ; Enzyme engineering ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. Technologies ; microsomes ; Miscellaneous ; NADP (coenzyme) ; photosynthesis ; photosystem I ; photosystem II ; rats ; recombinant proteins ; reduction ; Saccharomyces cerevisiae ; Spinacia oleracea ; unspecific monooxygenase</subject><ispartof>Biotechnology techniques, 1996-10, Vol.10 (10), p.717-720</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c313t-e990ab9bb0d9b4bccb2afe694dd8d457a0c91353441d1b63d6dd59ea42a5ab203</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2502626$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Y.S</creatorcontrib><creatorcontrib>Hara, M</creatorcontrib><creatorcontrib>Ikebukuro, K</creatorcontrib><creatorcontrib>Miyake, J</creatorcontrib><creatorcontrib>Ohkawa, H</creatorcontrib><creatorcontrib>Karube, I</creatorcontrib><title>Photo-induced activation of cytochrome P450/reductase fusion enzyme coupled with spinach chloroplasts</title><title>Biotechnology techniques</title><description>Photoactivation of cytochrome P450 monooxygenase was studied using a combination of spinach chloroplasts and yeast microsomes containing rat P4501A1/yeast reductase fusion enzyme. Under illumination, in the reaction mixture, NADP was reduced, transferring electrons to the P450/reductase fusion enzyme to convert 7-ethoxycoumarin to 7-hydroxycoumarin.</description><subject>7-ethoxycoumarin</subject><subject>7-hydroxycoumarin</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>chemical reactions</subject><subject>chloroplasts</subject><subject>Clostridium pasteurianum</subject><subject>coumarins</subject><subject>cytochrome P-450</subject><subject>de-ethylation</subject><subject>electron transfer</subject><subject>Enzyme engineering</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>microsomes</subject><subject>Miscellaneous</subject><subject>NADP (coenzyme)</subject><subject>photosynthesis</subject><subject>photosystem I</subject><subject>photosystem II</subject><subject>rats</subject><subject>recombinant proteins</subject><subject>reduction</subject><subject>Saccharomyces cerevisiae</subject><subject>Spinacia oleracea</subject><subject>unspecific monooxygenase</subject><issn>0951-208X</issn><issn>1573-6784</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNpF0EtLxDAUBeAgCo6PjX_ALsSFUL15tZOlDo4KAwoquCu3SWornaYmqTL-ejvMoKu7uN85i0PICYVLCpBf3cwBGGNS8h0yoTLnaZZPxS6ZgJI0ZTB92ycHIXwAAAcQE2Kfahdd2nRm0NYkqGPzhbFxXeKqRK-i07V3S5s8CQlX3o4qYrBJNYS1sd3PanxqN_TtmP5uYp2EvulQ14muW-dd32KI4YjsVdgGe7y9h-R1fvsyu08Xj3cPs-tFqjnlMbVKAZaqLMGoUpRalwwrmylhzNQImSNoRbnkQlBDy4ybzBipLAqGEksG_JCcb3p77z4HG2KxbIK2bYuddUMoqFR8misxwosN1N6F4G1V9L5Zol8VFIr1ksX_kiM-27Zi0NhWHjvdhL8Ek8Aylo3sdMMqdAW--5G8PjOgHKgce5jiv0ohfZM</recordid><startdate>19961001</startdate><enddate>19961001</enddate><creator>Kim, Y.S</creator><creator>Hara, M</creator><creator>Ikebukuro, K</creator><creator>Miyake, J</creator><creator>Ohkawa, H</creator><creator>Karube, I</creator><general>Chapman and Hall</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19961001</creationdate><title>Photo-induced activation of cytochrome P450/reductase fusion enzyme coupled with spinach chloroplasts</title><author>Kim, Y.S ; Hara, M ; Ikebukuro, K ; Miyake, J ; Ohkawa, H ; Karube, I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c313t-e990ab9bb0d9b4bccb2afe694dd8d457a0c91353441d1b63d6dd59ea42a5ab203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>7-ethoxycoumarin</topic><topic>7-hydroxycoumarin</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>chemical reactions</topic><topic>chloroplasts</topic><topic>Clostridium pasteurianum</topic><topic>coumarins</topic><topic>cytochrome P-450</topic><topic>de-ethylation</topic><topic>electron transfer</topic><topic>Enzyme engineering</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>microsomes</topic><topic>Miscellaneous</topic><topic>NADP (coenzyme)</topic><topic>photosynthesis</topic><topic>photosystem I</topic><topic>photosystem II</topic><topic>rats</topic><topic>recombinant proteins</topic><topic>reduction</topic><topic>Saccharomyces cerevisiae</topic><topic>Spinacia oleracea</topic><topic>unspecific monooxygenase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Y.S</creatorcontrib><creatorcontrib>Hara, M</creatorcontrib><creatorcontrib>Ikebukuro, K</creatorcontrib><creatorcontrib>Miyake, J</creatorcontrib><creatorcontrib>Ohkawa, H</creatorcontrib><creatorcontrib>Karube, I</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biotechnology techniques</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Y.S</au><au>Hara, M</au><au>Ikebukuro, K</au><au>Miyake, J</au><au>Ohkawa, H</au><au>Karube, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photo-induced activation of cytochrome P450/reductase fusion enzyme coupled with spinach chloroplasts</atitle><jtitle>Biotechnology techniques</jtitle><date>1996-10-01</date><risdate>1996</risdate><volume>10</volume><issue>10</issue><spage>717</spage><epage>720</epage><pages>717-720</pages><issn>0951-208X</issn><eissn>1573-6784</eissn><coden>BTECE6</coden><abstract>Photoactivation of cytochrome P450 monooxygenase was studied using a combination of spinach chloroplasts and yeast microsomes containing rat P4501A1/yeast reductase fusion enzyme. Under illumination, in the reaction mixture, NADP was reduced, transferring electrons to the P450/reductase fusion enzyme to convert 7-ethoxycoumarin to 7-hydroxycoumarin.</abstract><cop>London</cop><pub>Chapman and Hall</pub><doi>10.1007/BF00222553</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0951-208X |
| ispartof | Biotechnology techniques, 1996-10, Vol.10 (10), p.717-720 |
| issn | 0951-208X 1573-6784 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15938794 |
| source | SpringerLink Journals |
| subjects | 7-ethoxycoumarin 7-hydroxycoumarin Biological and medical sciences Biotechnology chemical reactions chloroplasts Clostridium pasteurianum coumarins cytochrome P-450 de-ethylation electron transfer Enzyme engineering Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies microsomes Miscellaneous NADP (coenzyme) photosynthesis photosystem I photosystem II rats recombinant proteins reduction Saccharomyces cerevisiae Spinacia oleracea unspecific monooxygenase |
| title | Photo-induced activation of cytochrome P450/reductase fusion enzyme coupled with spinach chloroplasts |
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