Cobalt-dependent transcription of the nitrile hydratase gene in Rhodococcus rhodochrous M8
Abstract The effects of cobalt ions on the activities of Rhodococcus rhodochrous M8 enzymes for nitrile utilization, nitrile hydratase and amidase, were investigated. In contrast to amidase, synthesis of nitrile hydratase and its activity required cobalt ions in the growth medium. Northern blot anal...
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| Veröffentlicht in: | FEMS microbiology letters 1996-11, Vol.144 (2-3), p.191-195 |
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| container_title | FEMS microbiology letters |
| container_volume | 144 |
| creator | Pogorelova, Tatyana E. Ryabchenko, Ludmila E. Sunzov, Nicola I. Yanenko, Alexander S. |
| description | Abstract
The effects of cobalt ions on the activities of Rhodococcus rhodochrous M8 enzymes for nitrile utilization, nitrile hydratase and amidase, were investigated. In contrast to amidase, synthesis of nitrile hydratase and its activity required cobalt ions in the growth medium. Northern blot analysis showed that in the presence of cobalt ions, the level of mRNA for nitrile hydratase genes was several times higher than that under cobalt-limited conditions. It was assumed that the low nitrile hydratase activity in cells grown in the absence of cobalt ions is connected either with the weak expression of nitrile hydratase genes or with the rapid degradation of nitrile hydratase mRNA. |
| doi_str_mv | 10.1111/j.1574-6968.1996.tb08529.x |
| format | Article |
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The effects of cobalt ions on the activities of Rhodococcus rhodochrous M8 enzymes for nitrile utilization, nitrile hydratase and amidase, were investigated. In contrast to amidase, synthesis of nitrile hydratase and its activity required cobalt ions in the growth medium. Northern blot analysis showed that in the presence of cobalt ions, the level of mRNA for nitrile hydratase genes was several times higher than that under cobalt-limited conditions. It was assumed that the low nitrile hydratase activity in cells grown in the absence of cobalt ions is connected either with the weak expression of nitrile hydratase genes or with the rapid degradation of nitrile hydratase mRNA.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1996.tb08529.x</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amidase ; Cobalt ; Cobalt ion ; Gene expression ; Genes ; Ions ; Microbiology ; Nitrile hydratase ; Nitrile hydratase gene transcript ; Rhodococcus rhodochrous ; Transcription</subject><ispartof>FEMS microbiology letters, 1996-11, Vol.144 (2-3), p.191-195</ispartof><rights>Copyright © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V. 1996</rights><rights>Copyright © 1996 Federation of European Microbiological Societies. Published by Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3531-9a3baec9386938723ad0f89dfbb5cdbde206427abe744b0a24da584088fb1f133</citedby><cites>FETCH-LOGICAL-c3531-9a3baec9386938723ad0f89dfbb5cdbde206427abe744b0a24da584088fb1f133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1996.tb08529.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1996.tb08529.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids></links><search><creatorcontrib>Pogorelova, Tatyana E.</creatorcontrib><creatorcontrib>Ryabchenko, Ludmila E.</creatorcontrib><creatorcontrib>Sunzov, Nicola I.</creatorcontrib><creatorcontrib>Yanenko, Alexander S.</creatorcontrib><title>Cobalt-dependent transcription of the nitrile hydratase gene in Rhodococcus rhodochrous M8</title><title>FEMS microbiology letters</title><description>Abstract
The effects of cobalt ions on the activities of Rhodococcus rhodochrous M8 enzymes for nitrile utilization, nitrile hydratase and amidase, were investigated. In contrast to amidase, synthesis of nitrile hydratase and its activity required cobalt ions in the growth medium. Northern blot analysis showed that in the presence of cobalt ions, the level of mRNA for nitrile hydratase genes was several times higher than that under cobalt-limited conditions. It was assumed that the low nitrile hydratase activity in cells grown in the absence of cobalt ions is connected either with the weak expression of nitrile hydratase genes or with the rapid degradation of nitrile hydratase mRNA.</description><subject>Amidase</subject><subject>Cobalt</subject><subject>Cobalt ion</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Ions</subject><subject>Microbiology</subject><subject>Nitrile hydratase</subject><subject>Nitrile hydratase gene transcript</subject><subject>Rhodococcus rhodochrous</subject><subject>Transcription</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqVkEtLAzEUhYMoWKv_ISi4mzGZzCNxIUjxBRVBdOMm5DV2yjQZkxTtvze1xYXowkDIhZxz7uED4BijHKdzNs9x1ZRZzWqaY8bqPEpEq4LlHztg9P21C0aINDTDiDX74CCEOUKoLFA9Ai8TJ0UfM20GY7WxEUYvbFC-G2LnLHQtjDMDbRd91xs4W2kvoggGvhprYGfh48xpp5xSywD91zzzLs339BDstaIP5mj7jsHz9dXT5DabPtzcTS6nmSIVwRkTRAqjGKF1uk1BhEYtZbqVslJaapN6lkUjpGnKUiJRlFpUtESUthK3mJAxON3kDt69LU2IfNEFZfpeWJOacFwxggvcJOHJD-HcLb1N3XhBCKaorFOjMTjfqJR3IXjT8sF3C-FXHCO-hs7nfE2Wr8nyNXS-hc4_kvliY35PtFb_cPLr-ylmOAVUmwC3HP6wZ78t_gQm0Zqz</recordid><startdate>19961101</startdate><enddate>19961101</enddate><creator>Pogorelova, Tatyana E.</creator><creator>Ryabchenko, Ludmila E.</creator><creator>Sunzov, Nicola I.</creator><creator>Yanenko, Alexander S.</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>19961101</creationdate><title>Cobalt-dependent transcription of the nitrile hydratase gene in Rhodococcus rhodochrous M8</title><author>Pogorelova, Tatyana E. ; Ryabchenko, Ludmila E. ; Sunzov, Nicola I. ; Yanenko, Alexander S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3531-9a3baec9386938723ad0f89dfbb5cdbde206427abe744b0a24da584088fb1f133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amidase</topic><topic>Cobalt</topic><topic>Cobalt ion</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Ions</topic><topic>Microbiology</topic><topic>Nitrile hydratase</topic><topic>Nitrile hydratase gene transcript</topic><topic>Rhodococcus rhodochrous</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pogorelova, Tatyana E.</creatorcontrib><creatorcontrib>Ryabchenko, Ludmila E.</creatorcontrib><creatorcontrib>Sunzov, Nicola I.</creatorcontrib><creatorcontrib>Yanenko, Alexander S.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pogorelova, Tatyana E.</au><au>Ryabchenko, Ludmila E.</au><au>Sunzov, Nicola I.</au><au>Yanenko, Alexander S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cobalt-dependent transcription of the nitrile hydratase gene in Rhodococcus rhodochrous M8</atitle><jtitle>FEMS microbiology letters</jtitle><date>1996-11-01</date><risdate>1996</risdate><volume>144</volume><issue>2-3</issue><spage>191</spage><epage>195</epage><pages>191-195</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Abstract
The effects of cobalt ions on the activities of Rhodococcus rhodochrous M8 enzymes for nitrile utilization, nitrile hydratase and amidase, were investigated. In contrast to amidase, synthesis of nitrile hydratase and its activity required cobalt ions in the growth medium. Northern blot analysis showed that in the presence of cobalt ions, the level of mRNA for nitrile hydratase genes was several times higher than that under cobalt-limited conditions. It was assumed that the low nitrile hydratase activity in cells grown in the absence of cobalt ions is connected either with the weak expression of nitrile hydratase genes or with the rapid degradation of nitrile hydratase mRNA.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6968.1996.tb08529.x</doi><tpages>5</tpages></addata></record> |
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| ispartof | FEMS microbiology letters, 1996-11, Vol.144 (2-3), p.191-195 |
| issn | 0378-1097 1574-6968 |
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| source | Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Amidase Cobalt Cobalt ion Gene expression Genes Ions Microbiology Nitrile hydratase Nitrile hydratase gene transcript Rhodococcus rhodochrous Transcription |
| title | Cobalt-dependent transcription of the nitrile hydratase gene in Rhodococcus rhodochrous M8 |
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