Continuous Microspectrophotometric Measurement of DNA Polymerase Activity: Application to the Klenow Fragment of Escherichia coli DNA Polymerase I and Human Immunodeficiency Virus Type 1 Reverse Transcriptase

Progress of DNA- and/or RNA-directed DNA polymerization reactions can be measured continuously using circular dichroism (CD) or ultraviolet (UV) spectroscopy. In the presence of the Klenow fragment of Escherichia coli DNA polymerase I, a CD change of -0.27 ± 0.06 millidegree at 248 nm and a UV chang...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1991-02, Vol.88 (3), p.1014-1018
Hauptverfasser:	Baillon, Jean G., Nashed, Nashaat T., Sayer, Jane M., Jerina, Donald M.
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Analytical, structural and metabolic biochemistry Base Sequence Biochemistry Biological and medical sciences Circular Dichroism DNA DNA Polymerase I - metabolism DNA, Bacterial - biosynthesis DNA, Viral - biosynthesis Enzymes Enzymes and enzyme inhibitors Escherichia coli Escherichia coli - enzymology Fundamental and applied biological sciences. Psychology HIV 1 HIV-1 - enzymology human immunodeficiency virus 1 Kinetics Molecular Sequence Data Nucleosides Nucleotides Oligonucleotides Peptide Fragments - metabolism Polymerization RNA-Directed DNA Polymerase - metabolism Spectrophotometry, Ultraviolet - methods Teeth Templates, Genetic Transferases
Online-Zugang:	Volltext
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