Alanine Exchanges of Polar Amino Acids in the Transmembrane Domains of a Platelet-activating Factor Receptor Generate Both Constitutively Active and Inactive Mutants

To determine ligand-binding sites of a platelet-activating factor (PAF) receptor, alanine-scanning mutagenesis was carried out. All 23 polar amino acids in the putative 7-transmembrane (TM) domains of a guinea pig PAF receptor were individually replaced with alanine. The ligand-binding properties of...

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Veröffentlicht in:	The Journal of biological chemistry 1997-03, Vol.272 (12), p.7846-7854
Hauptverfasser:	Ishii, Isao, Izumi, Takashi, Tsukamoto, Hiroaki, Umeyama, Hideaki, Ui, Michio, Shimizu, Takao
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine - chemistry Amino Acid Sequence Amino Acids - chemistry Animals CHO Cells COS Cells Cricetinae Membrane Proteins - chemistry Membrane Proteins - genetics Membrane Proteins - metabolism Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Platelet Activating Factor - metabolism Platelet Membrane Glycoproteins - chemistry Platelet Membrane Glycoproteins - genetics Platelet Membrane Glycoproteins - metabolism Protein Binding Receptors, Cell Surface Receptors, G-Protein-Coupled Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Homology, Amino Acid Signal Transduction Zinc - metabolism
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