Embryolethality of new herbicides is not detected by the micromass teratogen tests
New herbicidal compounds (11 pyrimidinediones, 3 benzoates and 1 sulfonamide) were found to be embryolethal but not teratogenic in rats. The range of the embryolethal dose varied from 0.2 to > 200 mg/kg. This broad range enabled us to validate whether proposed in vitro teratogen tests can detect...
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| Veröffentlicht in: | Archives of toxicology 1991-01, Vol.65 (2), p.145-149 |
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| creator | Tsuchiya, T Burgin, H Tsuchiya, M Winternitz, P Kistler, A |
| description | New herbicidal compounds (11 pyrimidinediones, 3 benzoates and 1 sulfonamide) were found to be embryolethal but not teratogenic in rats. The range of the embryolethal dose varied from 0.2 to > 200 mg/kg. This broad range enabled us to validate whether proposed in vitro teratogen tests can detect the embryolethality of these herbicides. The IC50 values (inhibition concentration 50%) for both differentiation and proliferation of midbrain and limb bud cells of rat embryos were determined and found to be above 50 micrograms/ml in all cases, confirming that the herbicides were not teratogenic. No correlation, however, was observed between the embryolethality in vivo and the activities in these cells. In order to test whether the potential to cause embryolethality could be predicted and detected as a general cytotoxic effect, the inhibition of colony forming ability in V79 cells was determined. The results indicated that cytotoxicity in V79 cells may be useful for preliminary testing of the embryolethal effect of herbicides. |
| doi_str_mv | 10.1007/BF02034942 |
| format | Article |
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The range of the embryolethal dose varied from 0.2 to > 200 mg/kg. This broad range enabled us to validate whether proposed in vitro teratogen tests can detect the embryolethality of these herbicides. The IC50 values (inhibition concentration 50%) for both differentiation and proliferation of midbrain and limb bud cells of rat embryos were determined and found to be above 50 micrograms/ml in all cases, confirming that the herbicides were not teratogenic. No correlation, however, was observed between the embryolethality in vivo and the activities in these cells. In order to test whether the potential to cause embryolethality could be predicted and detected as a general cytotoxic effect, the inhibition of colony forming ability in V79 cells was determined. The results indicated that cytotoxicity in V79 cells may be useful for preliminary testing of the embryolethal effect of herbicides.</description><identifier>ISSN: 0340-5761</identifier><identifier>EISSN: 1432-0738</identifier><identifier>DOI: 10.1007/BF02034942</identifier><identifier>PMID: 2059155</identifier><identifier>CODEN: ARTODN</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Abnormalities, Drug-Induced ; Animals ; benzoates ; Biological and medical sciences ; cell culture ; cell lines ; Cells, Cultured ; correlation ; Cricetinae ; cytotoxicity ; embryo (animal) ; Embryo, Mammalian - drug effects ; Embryology: invertebrates and vertebrates. Teratology ; embryotoxicity ; Extremities - embryology ; Fundamental and applied biological sciences. Psychology ; hamsters ; herbicides ; Herbicides - toxicity ; in vivo studies ; Mesencephalon - embryology ; pyrimidinediones ; Rats ; sulfonamides ; teratogenesis ; Teratology. Teratogens</subject><ispartof>Archives of toxicology, 1991-01, Vol.65 (2), p.145-149</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-eb6b55ff346e48643ce4165fceccd7944f98d5cba17325691e66852d65c988693</citedby><cites>FETCH-LOGICAL-c367t-eb6b55ff346e48643ce4165fceccd7944f98d5cba17325691e66852d65c988693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19614228$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2059155$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsuchiya, T</creatorcontrib><creatorcontrib>Burgin, H</creatorcontrib><creatorcontrib>Tsuchiya, M</creatorcontrib><creatorcontrib>Winternitz, P</creatorcontrib><creatorcontrib>Kistler, A</creatorcontrib><title>Embryolethality of new herbicides is not detected by the micromass teratogen tests</title><title>Archives of toxicology</title><addtitle>Arch Toxicol</addtitle><description>New herbicidal compounds (11 pyrimidinediones, 3 benzoates and 1 sulfonamide) were found to be embryolethal but not teratogenic in rats. The range of the embryolethal dose varied from 0.2 to > 200 mg/kg. This broad range enabled us to validate whether proposed in vitro teratogen tests can detect the embryolethality of these herbicides. The IC50 values (inhibition concentration 50%) for both differentiation and proliferation of midbrain and limb bud cells of rat embryos were determined and found to be above 50 micrograms/ml in all cases, confirming that the herbicides were not teratogenic. No correlation, however, was observed between the embryolethality in vivo and the activities in these cells. In order to test whether the potential to cause embryolethality could be predicted and detected as a general cytotoxic effect, the inhibition of colony forming ability in V79 cells was determined. The results indicated that cytotoxicity in V79 cells may be useful for preliminary testing of the embryolethal effect of herbicides.</description><subject>Abnormalities, Drug-Induced</subject><subject>Animals</subject><subject>benzoates</subject><subject>Biological and medical sciences</subject><subject>cell culture</subject><subject>cell lines</subject><subject>Cells, Cultured</subject><subject>correlation</subject><subject>Cricetinae</subject><subject>cytotoxicity</subject><subject>embryo (animal)</subject><subject>Embryo, Mammalian - drug effects</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>embryotoxicity</subject><subject>Extremities - embryology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hamsters</subject><subject>herbicides</subject><subject>Herbicides - toxicity</subject><subject>in vivo studies</subject><subject>Mesencephalon - embryology</subject><subject>pyrimidinediones</subject><subject>Rats</subject><subject>sulfonamides</subject><subject>teratogenesis</subject><subject>Teratology. Teratogens</subject><issn>0340-5761</issn><issn>1432-0738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMtLAzEQxoMoWh8X72IuehBW887mqFIfIAhqz0s2O7Er-6hJivS_N9JiTzPM9-ObmQ-hU0quKSH65u6BMMKFEWwHTajgrCCal7tokoekkFrRA3QY4xchlJWG76N9RqShUk7Q27Svw2rsIM1t16YVHj0e4AfPIdStaxuIuI14GBNuIIFL0OB6hdMccN-6MPY2Rpwg2DR-wpC7mOIx2vO2i3CyqUdo9jD9uH8qXl4fn-9vXwrHlU4F1KqW0nsuFIhSCe5AUCW9A-cabYTwpmykqy3VnEllKChVStYo6UxZKsOP0OXadxHG72XeXPVtdNB1doBxGSsqDRNaswxercF8cIwBfLUIbW_DqqKk-guw2gaY4bON67LuoflHN4ll_WKj2-hs54MdXBu3jkZRwViZufM15-1Y2c-Qmdk7I5ST_I_QhPNfEbiAhw</recordid><startdate>19910101</startdate><enddate>19910101</enddate><creator>Tsuchiya, T</creator><creator>Burgin, H</creator><creator>Tsuchiya, M</creator><creator>Winternitz, P</creator><creator>Kistler, A</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T2</scope><scope>7TV</scope><scope>7U2</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19910101</creationdate><title>Embryolethality of new herbicides is not detected by the micromass teratogen tests</title><author>Tsuchiya, T ; Burgin, H ; Tsuchiya, M ; Winternitz, P ; Kistler, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-eb6b55ff346e48643ce4165fceccd7944f98d5cba17325691e66852d65c988693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Abnormalities, Drug-Induced</topic><topic>Animals</topic><topic>benzoates</topic><topic>Biological and medical sciences</topic><topic>cell culture</topic><topic>cell lines</topic><topic>Cells, Cultured</topic><topic>correlation</topic><topic>Cricetinae</topic><topic>cytotoxicity</topic><topic>embryo (animal)</topic><topic>Embryo, Mammalian - drug effects</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>embryotoxicity</topic><topic>Extremities - embryology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hamsters</topic><topic>herbicides</topic><topic>Herbicides - toxicity</topic><topic>in vivo studies</topic><topic>Mesencephalon - embryology</topic><topic>pyrimidinediones</topic><topic>Rats</topic><topic>sulfonamides</topic><topic>teratogenesis</topic><topic>Teratology. Teratogens</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsuchiya, T</creatorcontrib><creatorcontrib>Burgin, H</creatorcontrib><creatorcontrib>Tsuchiya, M</creatorcontrib><creatorcontrib>Winternitz, P</creatorcontrib><creatorcontrib>Kistler, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Pollution Abstracts</collection><collection>Safety Science and Risk</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Archives of toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsuchiya, T</au><au>Burgin, H</au><au>Tsuchiya, M</au><au>Winternitz, P</au><au>Kistler, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Embryolethality of new herbicides is not detected by the micromass teratogen tests</atitle><jtitle>Archives of toxicology</jtitle><addtitle>Arch Toxicol</addtitle><date>1991-01-01</date><risdate>1991</risdate><volume>65</volume><issue>2</issue><spage>145</spage><epage>149</epage><pages>145-149</pages><issn>0340-5761</issn><eissn>1432-0738</eissn><coden>ARTODN</coden><abstract>New herbicidal compounds (11 pyrimidinediones, 3 benzoates and 1 sulfonamide) were found to be embryolethal but not teratogenic in rats. The range of the embryolethal dose varied from 0.2 to > 200 mg/kg. This broad range enabled us to validate whether proposed in vitro teratogen tests can detect the embryolethality of these herbicides. The IC50 values (inhibition concentration 50%) for both differentiation and proliferation of midbrain and limb bud cells of rat embryos were determined and found to be above 50 micrograms/ml in all cases, confirming that the herbicides were not teratogenic. No correlation, however, was observed between the embryolethality in vivo and the activities in these cells. In order to test whether the potential to cause embryolethality could be predicted and detected as a general cytotoxic effect, the inhibition of colony forming ability in V79 cells was determined. The results indicated that cytotoxicity in V79 cells may be useful for preliminary testing of the embryolethal effect of herbicides.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>2059155</pmid><doi>10.1007/BF02034942</doi><tpages>5</tpages></addata></record> |
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| ispartof | Archives of toxicology, 1991-01, Vol.65 (2), p.145-149 |
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| language | eng |
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| subjects | Abnormalities, Drug-Induced Animals benzoates Biological and medical sciences cell culture cell lines Cells, Cultured correlation Cricetinae cytotoxicity embryo (animal) Embryo, Mammalian - drug effects Embryology: invertebrates and vertebrates. Teratology embryotoxicity Extremities - embryology Fundamental and applied biological sciences. Psychology hamsters herbicides Herbicides - toxicity in vivo studies Mesencephalon - embryology pyrimidinediones Rats sulfonamides teratogenesis Teratology. Teratogens |
| title | Embryolethality of new herbicides is not detected by the micromass teratogen tests |
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